Topochemical investigation into the delignification of Eucalyptus globulus chips during semi-chemical sulfite pulping

Enzymatic digestion of alkaline‐sulfite pretreated sugar cane bagasse and its correlation with the chemical and structural changes occurring during the pretreatment step

“…Previous studies evaluating the lignocellulose recalcitrance indicate that the cell wall porosity and the fiber hydrophilicity are key factors governing the efficiency of the enzymatic hydrolysis of the polysaccharides contained in lignocellulosic materials . For sulfite pretreatments, there are evidences indicating that sulfonation of residual lignins increases the material's hydrophilicity, enhancing the digestibility of the pretreated material . In the present work, the WRV, the total acid contents and the fiber widths were determined in the pretreated materials, since these characteristics could give information on the substrate hydrophilicity or the capacity of the fibers to hold water and swell up (Table ).…”

Steam pretreatment for enzymatic hydrolysis of poplar wood: comparison of optimal conditions with and without SO2 impregnation

“…Another well-established technique for a semiquantitative localization of lignin and phenolic extractives within individual cell types and cell wall layers is provided by UV microspectrophotometry (UMSP) (Fergus et al 1969 ;Saka et al 1982 ;Koch and Kleist 2001 ). Different applications of this method demonstrate that UMSP is ideally suited to study the delignifi cation of wood chips during pulping processes (Koch et al 2003 ;Rehbein et al 2010 ). Siqueira et al (2011) correlated the topochemical distribution of lignin and hydroxycinnamic acids in sugar cane cell walls with the enzymatic hydrolysis of polysaccharides by using scanning UV spectroscopy.…”

Topochemical and transmission electron microscopic studies of bacterial decay in pine (Pinus sylvestris L.) harbour foundation piles

“…To obtain topochemical information on bacterial cell wall degradation, special techniques like UV-microspectrophotometry (UMSP) must be applied. This technique is a powerful tool to provide general information on the distribution of lignin and phenolic extractives within individual cell wall layers of various cell types Scott et al, 1969;Koch and Kleist, 2001;Koch et al, 2003b;Mendonç a et al, 2004;Singh et al, 2006;Kim et al, 2008;Rehbein et al, 2010).…”