Topographic Measurements of Real Structures in reflection Confocal Laser Scanning Microscope (CLSM)

Wessel, Sonja; Pagel, Sinja; Ritter, Martin; Hohenberg, Heinz; Wepf, Roger

doi:10.1017/s1431927603016246

Cited by 4 publications

(2 citation statements)

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“…This is advantageous because optical microscopy allows to screen large regions on the sample rapidly and then to increase magnifications for a more detailed inspection. CLSM measurements allow in addition the reconstruction of topographic information from recorded image stacks [58,59]. As an example, the reconstructed topography of the spot in Fig.…”

Section: Resultsmentioning

confidence: 99%

Scanning electrochemical microscopy study of laccase within a sol–gel processed silicate film

Nogala

Burchardt

Opałło

et al. 2008

Bioelectrochemistry

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Section: Resultsmentioning

confidence: 99%

Scanning electrochemical microscopy study of laccase within a sol–gel processed silicate film

Nogala

Burchardt

Opałło

et al. 2008

Bioelectrochemistry

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“…Briones and others (2006) also applied the SSFA to describe the surface roughness of bloomed chocolate. Wessel and others (2003) demonstrated that the unevenness and steep edges of an object may negatively impact the images obtained by reflection CLSM. However, they concluded that reflection CLSM is an underestimated, quantitative, topographic imaging tool and a real alternative compared to scanning electron microscopy (SEM) and atomic force microscopy (AFM) for surface texture measurement applications.…”

Section: Introductionmentioning

confidence: 99%

Food Surface Texture Measurement Using Reflective Confocal Laser Scanning Microscopy

Sheen

Bao

Cooke

2008

Journal of Food Science

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Confocal laser scanning microscopy (CLSM) was used in the reflection mode to characterize the surface texture (roughness) of sliced food surfaces. Sandpapers with grit size between 150 and 600 were used as height references to standardize the CLSM hardware settings. Sandpaper particle sizes were verified by scanning electron microscopy (SEM). The mean amplitude (in micrometers) of surface variation along line segments of the scanned sandpaper topographical image sets showed very close agreement between the measured result and the grit particle size (based on the U.S. Coated Abrasive Manufactures Inst. {CAMI], standard). The verified instrument settings were then used to measure the surface texture of mechanically sliced food surfaces, including cooked ham, salami, and cheese. Sliced food surface texture parameters of Ra (average height of a line segment), Rs (surface area ratio), Pa (average height on a region of interest), and Pq (root-mean-square height on a region of interest) were evaluated by this method. Values of the surface roughness of sliced ham, salami, and cheese were found to be comparable to the range of dimensions of selected sandpapers. The CLSM method may be useful for other surface texture measurements, and to investigate the impact of food surface texture on microbial adhesion or attachment, which might play a significant role in microbial transfer from one surface to another.

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Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging

Gang¹,

Liu²,

Wang³

et al. 2017

Biomed. Opt. Express

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High-resolution three-dimensional biomolecule distribution information of large samples is essential to understanding their biological structure and function. Here, we proposed a method combining large sample resin embedding with iDISCO immunofluorescence staining to acquire the profile of biomolecules with high spatial resolution. We evaluated the compatibility of plastic embedding with an iDISCO staining technique and found that the fluorophores and the neuronal fine structures could be well preserved in the Lowicryl HM20 resin, and that numerous antibodies and fluorescent tracers worked well upon Lowicryl HM20 resin embedding. Further, using fluorescence MicroOptical sectioning tomography (fMOST) technology combined with ultra-thin slicing and imaging, we were able to image the immunolabeled large-volume tissues with high resolution. Miyawaki, "Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain," Nat. Neurosci. 14(11), 1481-1488 (2011). 11. J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, "Optical projection tomography as a tool for 3D microscopy and gene expression studies," Science 296 (

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Topographic Measurements of Real Structures in reflection Confocal Laser Scanning Microscope (CLSM)

Cited by 4 publications

References 0 publications

Scanning electrochemical microscopy study of laccase within a sol–gel processed silicate film

Scanning electrochemical microscopy study of laccase within a sol–gel processed silicate film

Food Surface Texture Measurement Using Reflective Confocal Laser Scanning Microscopy

Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging

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