Topographical cues of biomaterials and fibroblast activation: Are they related?

Chen, Zuhan; Bi, Huanjing; Ding, Xiaoming

doi:10.1016/j.mbm.2023.100028

Cited by 1 publication

(2 citation statements)

References 22 publications

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“…This intricate process encompasses the initial adsorption of proteins onto the biomaterial surface followed by the recruitment and activation of inflammatory cells. Additionally, a foreign body reaction involves the activation of fibroblasts and the subsequent formation of a fibrous capsule surrounding the implanted material . It remains unclear how these two different forms of CaPs differentially regulate the growth of MSCs and fibroblasts.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, a foreign body reaction involves the activation of fibroblasts and the subsequent formation of a fibrous capsule surrounding the implanted material. 43 It remains unclear how these two different forms of CaPs differentially regulate the growth of MSCs and fibroblasts. It has been reported that HA particles significantly decreased the number and proliferation of fibroblasts, 44,45 plausibly by suppressing the protein production of transforming growth factor beta-1 (TGF-β1) and inducing the synthesis of prostaglandin E2 (PGE2).…”

Section: Growth Of Human Mscs and Fibroblasts On Composite Bone Cementsmentioning

confidence: 99%

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In Vitro Osteo-Immunological Responses of Bioactive Calcium Phosphate-Containing Urethane Dimethacrylate-Based Composites: A Potential Alternative to Poly(methyl methacrylate) Bone Cement

Singhatanadgit,

Sungkhaphan,

Thavornyutikarn

et al. 2024

ACS Mater. Au

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This investigation developed new composite bone cements using urethane dimethacrylate (UDMA), poly(propylene glycol) dimethacrylate (PPGDMA), and hydroxyethyl methacrylate (HEMA), with micrometer-sized aluminosilicate glass filler. Monocalcium phosphate monohydrate (MCPM) and hydroxyapatite (HA) particles were added to enhance biological performance, particularly osteo-immunomodulation. Free radical polymerization was triggered by mixing two pastes containing either benzoyl peroxide (BPO, an initiator) or N-tolyglycine glycidyl methacrylate (NTGGMA, an activator). Increasing butylated hydroxytoluene (BHT, an inhibitor) enabled a suitable delay after mixing at 25 °C for placement. At 37 °C, the delay time was reduced and the final conversion was enhanced. Findings also demonstrated the biocompatibility of the developed bone cement toward osteo-immunological cell lineages, including mesenchymal stem cells (MSCs), fibroblasts, osteoclast precursor RAW 246.7 cells, and peripheral blood mononuclear cells (PBMCs). Notably, the cement with both MCPM and HA combined facilitated sufficient MSC growth, enabling subsequent mineralization while concurrently suppressing the proliferation of fibroblasts, osteoclast progenitors, and PBMCs. Furthermore, composite cement exhibited the capacity to differentially regulate osteoblast differentiation, cell-(in)dependent mineralization, osteoclastogenesis, and PBMC-mediated inflammatory responses at both cellular and molecular levels in vitro. These observations suggested their potential use for bone repair, especially in cases of inflammation-associated bone defects.

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Section: Resultsmentioning

confidence: 99%