Topographical Distribution of NADPH-Diaphorase-Positive Neurons in the Cat?s Claustrum

Hinova-Palova, Dimka; Paloff, Adrian; Christova, Tatjana; Ovtscharoff, Wladimir

doi:10.1076/ejom.35.2.105.13068

Cited by 18 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our present data are in a good agreement with our previous works (Hinova-Palova et al 1997) where the throughout distribution of NADPHd positive neurons in the DC of cat was reported. In spite of the good correlation between both NADPHd staining and nNOS-ir (Dawson et al 1991b;Hope et al 1991;Lysakowski and Singer 2000), some remarks must be made: First, the NADPHd staining results in significant background staining and it reveals more neuronal processes with axonal appearance.…”

Section: Discussionsupporting

confidence: 96%

“…In fact according to the best of our knowledge there is a single work describing the nNOS-ir in the DC of a cat (Rahman and Baizer 2007). In one of our previous works we reported the morphology and distribution of NADPH diaphorase (NADPHd) positive neurons in DC of a cat (Hinova-Palova et al 1997). Some of present data were already reported (Hinova-Palova et al 2005).…”

Section: Introductionsupporting

confidence: 55%

“…It was shown that it takes part in many processes in the central nervous system like synaptic plasticity in the hippocampus (Krushkov et al 1996), claustral functioning (Hinova-Palova et al 1997), eye movements (Moreno-Lopez et al 1996, thalamic functioning (Krushkov et al 1996), blood pressure control (Maeda et al 1999), inferior colliculus functioning , vestibular complex functioning (Papantchev et al, , 2006 and so forth. NO is synthesized by the enzyme Nitric oxide synthase (NOS).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study

Hinova-Palova

Edelstein²,

Paloff

et al. 2008

J Mol Hist

View full text Add to dashboard Cite

Nitric oxide is a unique neurotransmitter, which participates in many physiological and pathological processes in the organism. Nevertheless there are little data about the neuronal Nitric Oxide Synthase immunoreactive (nNOS-ir) neurons and fibers in the dorsal claustrum (DC) of a cat. In this respect the aims of this study were: (1) to demonstrate nNOS-ir in the neurons and fibers of the DC; (2) to describe their light microscopic morphology and distribution; (3) to investigate and analyze the ultrastructure of the nNOS-ir neurons, fibers and synaptic terminals; (4) to verify whether the nNOS-ir neurons consist a specific subpopulation of claustral neurons; (5) to verify whether the nNOS-ir neurons have a specific pattern of organization throughout the DC. For demonstration of the nNOS-ir the Avidin-Biotin-Peroxidase Complex method was applied. Immunopositive for nNOS neurons and fibers were present in all parts of DC. On the light microscope level nNOS-ir neurons were different in shape and size. According to the latter they were divided into three groups-small (with diameter under 15 microm), medium-sized (with diameter from 16 to 20 microm) and large (with diameter over 21 microm). Some of nNOS-ir neurons were lightly-stained while others were darkly-stained. On the electron microscope level the immunoproduct was observed in neurons, dendrites and terminal boutons. Different types of nNOS-ir neurons differ according to their ultrastructural features. Three types of nNOS-ir synaptic boutons were found. As a conclusion we hope that the present study will contribute to a better understanding of the functioning of the DC in cat and that some of the data presented could be extrapolated to other mammals, including human.

show abstract

Section: Discussionsupporting

confidence: 96%

Section: Introductionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study

Hinova-Palova

Edelstein²,

Paloff

et al. 2008

J Mol Hist

View full text Add to dashboard Cite

show abstract

“…Hinova-Palova et al showed that there are two populations of NADPH-dr neurons in the CL. One population, consisting of large and medium-sized positive neurons, represents projection neurons, while the other population of small positive neurons corresponds to local circuit neurons [13] and only the densely stained cells were GABA immunoreactive [11]. In our experiments, it was found that a large number of NADPH-dr neurons are localized in Pu, especially in the ventral part.…”

Section: Discussionsupporting

confidence: 46%

Changes of c-Fos expression and NADPH-d activity in claustrum induced by chronic muscle inflammation in cat (a preliminary study)

2022

View full text Add to dashboard Cite

An investigation of the central mechanisms underlying muscle inflammation and musculoskeletal pain is an important step to find means for the prevention or treatment of muscle inflammation. One of the insufficiently studied brain structures involved in the transmission of nociceptive information is the claustrum (CL). Therefore, the aim of the study was to reveal changes in the patterns of Fos-immunoreactivity and NADPH-diaphoreactivity in the nucleus claustrum (CL) and additionally in the ventral putamen (Pu) during chronic inflammation of m. gastrocnemius-soleus in cat, induced by intramuscular injection of complete Freund’s adjuvant (CFA). Immunohistochemical and histochemical techniques were used to detect Fos-immunoreactive (Fos-ir) and NADPH-diaphorase reactive (NADPH-dr) neurons within studied structures. It was revealed that nine days after CFA-induced muscle inflammation the level of Fos-immunoreactivity and NADPH-d reactivity within the CL and in the ventral part of Pu increased two-fold in comparison with the control. Because the CL is reciprocally connected with many structures of the brain cortex and subcortical structures, all these structures can be pathways of transmission of nociceptive information, nevertheless, it can be assume that the central amygdala nucleus may make the main nociceptive contribution to the activation of neurons within the CL. It is known that CL is mutually related to Pu, but it was not possible to assess their mutual influence in this study. The results of the study of the Fos-ir neurons distribution in CL and Pu under conditions of long-term muscles inflammation indicate the active involvement of the mentioned structures in the formation of adaptive reactions. The increase in the number of neurons with NADPH-d reactivity in CL and Pu indicates that NO-signals play a significant role in the formation and amplification of the response to painful impulses from inflamed muscles. In addition, further research is needed to accurately identify all possible nociceptive inputs to the CL and to separate the emotional, motor, auditory, and visual components that may accompany nociceptive processes.

show abstract

“…The insular claustrum and endopiriform nucleus contain populations of projection neurons with polymorphous perikarya and spiny dendrites [Brand, 1981;LeVay and Sherk, 1981] some of which may be NADPH-diaphorase positive [Hinova-Palova et al, 1997], as well as interneurons [Braak and Braak, 1982] which are immunoreactive for calcium binding proteins [Druga et al, 1993;Kowianski et al, 2004] and are characterized by round or ovoid perikarya and aspinous, beaded dendrites [Reynhout and Baizer, 1999]. In some species complementarity of immunoreactivity for parvalbumin (Pv) and calretinin (Cr) has been observed in the insular claustrum, with a strongly Pv immunoreactive region corresponding in position within the insular claustrum to a Cr immunonegative region [Druga et al, 1993;Paxinos et al, 1999;Real et al, 2003].…”

Section: Introductionmentioning

confidence: 99%

The Claustrum Is Not Missing from All Monotreme Brains

2004

View full text Add to dashboard Cite

Many authors have reported that the claustrum, which comprises the insular claustrum and the endopiriform nucleus, is missing from the monotreme forebrain. We used Nissl and myelin staining in conjunction with enzyme histochemistry for acetylcholinesterase and immunohistochemistry for parvalbumin, calbindin, calretinin and tyrosine hydroxylase to examine the brains of two monotremes, the short-beaked echidna (Tachyglossus aculeatus) and the platypus (Ornithorhynchus anatinus). We found that although the insular claustrum is a small structure in the echidna brain, it is nevertheless clearly present as loosely clustered neurons embedded in the white matter ventrolateral to the putamen and deep to the piriform and entorhinal cortices. Neurons in this region share the chemical features of the adjacent cortex (presence of a similar proportion of parvalbumin immunoreactive neurons and minimal activity for acetylcholinesterase and tyrosine hydroxylase), unlike the adjacent putamen and ventral pallidum. A putative endopiriform nucleus can be identified in the interior of the piriform lobe of the echidna as calretinin immunoreactive neurons embedded within the white matter. The situation is much less clear in the platypus, but our data suggest that there may be an insular claustrum deep to frontal cortex, separated from layer VI by only a thin layer of white matter. We could not identify an endopiriform nucleus in our platypus material. Our findings indicate that presence of the claustrum cannot be considered a feature confined to therian mammals and lend weight to arguments that this structure was present in the ancestral mammalian brain.

show abstract

Topographical Distribution of NADPH-Diaphorase-Positive Neurons in the Cat?s Claustrum

Cited by 18 publications

References 0 publications

Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study

Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study

Changes of c-Fos expression and NADPH-d activity in claustrum induced by chronic muscle inflammation in cat (a preliminary study)

The Claustrum Is Not Missing from All Monotreme Brains

Contact Info

Product

Resources

About