2009
DOI: 10.1007/s00418-009-0623-z
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Topographical variation in the distributions of versican, aggrecan and perlecan in the foetal human spine reflects their diverse functional roles in spinal development

Abstract: We evaluated the immunohistochemical distribution of three major proteoglycans of cartilage, i.e., aggrecan, versican and perlecan vis-a-vis collagens I and II in the developing human spine of first-trimester foetuses. Aggrecan and perlecan were prominently immunolocalised in the cartilaginous vertebral body rudiments and to a lesser extent within the foetal intervertebral disc. In contrast, versican was only expressed in the developing intervertebral disc interspace. Using domain-specific monoclonal antibodie… Show more

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Cited by 39 publications
(39 citation statements)
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“…The collected evidence presented herein therefore strongly supports our assertion that perlecan should be considered as a cellassociated chondrogenic marker. Perlecan is detectable in very early stages of cartilage development (Gustafsson et al 2003;Hassell et al 2002;Melrose et al 2002, 2004b, 2008a, Roediger et al 2009Smith et al 2009) and, although diminished in content in mature cartilages partly due to the age-dependant decline in cell numbers in this tissue, it is nevertheless still a readily detectable cellassociated entity (Melrose et al 2005b(Melrose et al , 2006. Indeed, in chondrocyte clones in osteoarthritic cartilage, perlecan expression has been shown to be focally up-regulated compared to that in the resident permanent chondrocytes Miosge 2004, 2005).…”
Section: Discussionmentioning
confidence: 91%
“…The collected evidence presented herein therefore strongly supports our assertion that perlecan should be considered as a cellassociated chondrogenic marker. Perlecan is detectable in very early stages of cartilage development (Gustafsson et al 2003;Hassell et al 2002;Melrose et al 2002, 2004b, 2008a, Roediger et al 2009Smith et al 2009) and, although diminished in content in mature cartilages partly due to the age-dependant decline in cell numbers in this tissue, it is nevertheless still a readily detectable cellassociated entity (Melrose et al 2005b(Melrose et al , 2006. Indeed, in chondrocyte clones in osteoarthritic cartilage, perlecan expression has been shown to be focally up-regulated compared to that in the resident permanent chondrocytes Miosge 2004, 2005).…”
Section: Discussionmentioning
confidence: 91%
“…Collation of biometric data from these imaging studies has enabled the tabulation of reference values for the non-invasive assessment of normal human foetal spinal growth [2]. Histochemistry and immunohistochemistry has demonstrated the distribution of collagen types [5], including the alternatively spliced type IIA and IIB collagens [6], elastic microfibrillar and matrix proteins [7] and proteoglycans [8,9].…”
Section: Introductionmentioning
confidence: 99%
“…This multilevel structural uniformity suggests a consistency in the functional role played by these nodes throughout the life of the healthy disc. Although this paper has not analysed the composition of the nodes, the nucleus and inner annulus consist mostly of type II collagen [24][25][26] and thus is likely to be a primary nodal component. Further, in view of the more recent discovery of elastin in the nucleus [27,28], this too could also form part of the nodal structure but is outside the scope of the present study.…”
Section: Discussionmentioning
confidence: 99%