Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Cun, Xingli; Hosta‐Rigau, Leticia

doi:10.3390/nano10102070

Cited by 92 publications

(85 citation statements)

References 260 publications

(430 reference statements)

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“…The strategy of combining biomaterial scaffolds with cells can be directed to various tissue injuries depending on the cell types applied. One of the most explored cell types in tissue engineering is the mesenchymal stromal cell (MSC), due to its differentiation potential, various isolation sources, low immunogenicity and high immunomodulatory potential ( Li et al, 2019 ; Cun and Hosta-Rigau, 2020 ; Wang et al, 2021 ). Along with MSCs, endothelial cells and their progenitors, such as the expanded CD133 + cell, are also crucial to tissue engineering due to their angiogenic potential ( Bongiovanni et al, 2014 ) and paracrine activity ( Angulski et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

Biagini

Senegaglia

Pereira

et al. 2021

Front. Bioeng. Biotechnol.

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Tissue engineering is a branch of regenerative medicine, which comprises the combination of biomaterials, cells and other bioactive molecules to regenerate tissues. Biomaterial scaffolds act as substrate and as physical support for cells and they can also reproduce the extracellular matrix cues. Although tissue engineering applications in cellular therapy tend to focus on the use of specialized cells from particular tissues or stem cells, little attention has been paid to endothelial progenitors, an important cell type in tissue regeneration. We combined 3D printed poly(lactic acid) scaffolds comprising two different pore sizes with human adipose-derived stromal cells (hASCs) and expanded CD133+ cells to evaluate how these two cell types respond to the different architectures. hASCs represent an ideal source of cells for tissue engineering applications due to their low immunogenicity, paracrine activity and ability to differentiate. Expanded CD133+ cells were isolated from umbilical cord blood and represent a source of endothelial-like cells with angiogenic potential. Fluorescence microscopy and scanning electron microscopy showed that both cell types were able to adhere to the scaffolds and maintain their characteristic morphologies. The porous PLA scaffolds stimulated cell cycle progression of hASCs but led to an arrest in the G1 phase and reduced proliferation of expanded CD133+ cells. Also, while hASCs maintained their undifferentiated profile after 7 days of culture on the scaffolds, expanded CD133+ cells presented a reduction of the von Willebrand factor (vWF), which affected the cells’ angiogenic potential. We did not observe changes in cell behavior for any of the parameters analyzed between the scaffolds with different pore sizes, but the 3D environment created by the scaffolds had different effects on the cell types tested. Unlike the extensively used mesenchymal stem cell types, the 3D PLA scaffolds led to opposite behaviors of the expanded CD133+ cells in terms of cytotoxicity, proliferation and immunophenotype. The results obtained reinforce the importance of studying how different cell types respond to 3D culture systems when considering the scaffold approach for tissue engineering.

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Section: Introductionmentioning

confidence: 99%

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

Biagini

Senegaglia

Pereira

et al. 2021

Front. Bioeng. Biotechnol.

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“…In the same gure, note the di culty of nding the adhered cells being molded according to the surface on which they were exposed. Many works have found that cells have the ability to mold and modify their geometry depending on the environment [40].…”

Section: Discussionmentioning

confidence: 99%

Low Mg Content on Ti-Nb-Sn Alloy When in Contact with BMMSCs Promotes Improvement of its Biological Functions

Dias

Rossi

Apolonio

et al. 2021

Preprint

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Introduction: Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive.Objective: This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs).Methods: Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE).Results and discussion: The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.

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“…80 Ongoing studies exhibits cell's behavior ranging affects from the adhesion of cell fluctuations to intracellular signaling pathway regulation that controls transcriptional activity and gene expression. 81 Traditionally, when considering bulk tissue properties, the nature of materials did not span the full range of biological length scale topography considered to affect cell activity (ranging from 10 nm to 100 μm). Nanophase reinforcements (such as HA-collagen nanocomposites or carbon nanotube-polymer nanocomposites) are now producing advances in bioactivity and mechanical properties such as flexural and compressive modules.…”

Section: Enhancing Materials Bio-functionalitymentioning

confidence: 99%

Materials science and design principles of therapeutic materials in orthopedic and bone tissue engineering

Liang

Dong

Shen

et al. 2021

Polymers for Advanced Techs

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The successful materials design for bone-tissue engineering needs to understand the structure and composition of host bone tissue, and selecting appropriate biomimetic natural or tunable synthetic materials (biomaterials), such as polymers, bioceramics, metals, and composites. Materials that improve bone tissue engineering have tremendous potential for clinical applications to treat and regenerate various bone injuries and fractures. The drive to design bone grafts for bridging the gaps in orthopedic tissue regeneration results in a significant research thrust for designing and developing material for bone tissue regeneration. Recently, numerous challenges, including biomaterial designing, can well suit the biological, chemical and mechanical microenvironment of natural orthopedic tissues and adopt vascularization. Novel bio-inspired materials attempt to enhance the biofunctionality that reconstruct microlevel biofactor and topographical signals from the extracellular environment are rapidly developing. The most important paradigm shift in orthopedic tissue engineering is the careful selection of scaffold, signals, and cell types to design biomaterials. Notwithstanding, the clinical outcome of orthopedic tissue regeneration was so vibrant years ago; however, so far, it failed to achieve the expected results and failed to translate in the clinical setting. In the current review, we discuss the aims and mechanism of bone tissue regeneration, biomaterial designing, and orthopedic tissue engineering requirements. Next, we highlight the principle for orthopedic tissue engineering and describe the strategies for enhancing material Bio-functionality. Finally, we give the bench to bedside concept and concluding remarks with a special focus on future. The main purpose of current paper is to give a comprehensive overview, which is helpful for the researcher to distill this information and design future research strategies for designing materials for orthopedic and bone tissue engineering.

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Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Cited by 92 publications

References 260 publications

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

Low Mg Content on Ti-Nb-Sn Alloy When in Contact with BMMSCs Promotes Improvement of its Biological Functions

Materials science and design principles of therapeutic materials in orthopedic and bone tissue engineering

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