Topography effects of pure titanium substrates on human osteoblast long-term adhesion

Anselme, Karine; Bigerelle, Maxence

doi:10.1016/j.actbio.2004.11.009

Cited by 281 publications

(230 citation statements)

References 38 publications

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“…In conclusion, the data suggest that implants with moderately rough surfaces, that have been developed to promote osteoblast adhesion (Anselme & Bigerelle, 2005) and differentiation of osteoblast progenitor cells (Wall et al, 2009), have, in themselves, a greater propensity for retention of adhered bacteria. Bacterial adherence on these surfaces would be compounded by the presence of a salivary film to which bacterial species/strains which express adhesins for salivary molecules can bind.…”

Section: Discussionmentioning

confidence: 87%

Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

et al. 2012

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Section: Discussionmentioning

confidence: 87%

Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

et al. 2012

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“…Several in vitro studies have reported the effects of surface roughness on the adhesion, proliferation, and differentiation of osteoblastic cells. Osteoblastic cells attach, spread, and proliferate more rapidly on smooth surfaces than on rough surfaces while their differentiation is enhanced by rough surfaces [2,4,17]. Both the early fixation and long-term mechanical stability of the prosthesis can be improved by a higher surface roughness.…”

Section: Discussionmentioning

confidence: 99%

“…The Ti6Al4 V plates were ground with abrasive papers, ultrasonically washed with acetone and distilled water, and dried at 40°C. A fresh electrolyte prepared by dissolving reagent-grade chemicals of Ca (CH 3 COO) 2 …”

Section: Methodsmentioning

confidence: 99%

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Lim

Kwon

Sun

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Microarc oxidation (MAO) is a surface treatment that provides nanoporous pits, and thick oxide layers, and incorporates calcium and phosphorus into the coating layer of titanium alloy. We presumed such modification on the surface of titanium alloy by MAO would improve the ability of cementless stems to osseointegrate. We therefore compared the in vitro ability of cells to adhere to MAOed titanium alloy to that of two different types of surface modifications: machined and grit-blasted. We performed energy-dispersive x-ray spectroscopy and scanned electron microscopy investigations to assess the structure and morphology of the surfaces. Biologic and morphologic responses to osteoblast cell lines (SaOS-2) were then examined by measuring cell proliferation, cell differentiation (alkaline phosphatase activity), and avb3 integrin. The cell proliferation rate, alkaline phosphatase activity, and cell adhesion in the MAO group increased in comparison to those in the machined and grit-blasted groups. The osteoblast cell lines of the MAO group were also homogeneously spread on the surface, strongly adhered, and well differentiated when compared to the other groups. This method could be a reasonable option for treating the surfaces of titanium alloy for better osseointegration.

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“…Osteoblasts exhibit a more differentiated phenotype when grown on such surfaces (see refs. 19 and 20 for reviews), resulting in a complex osteoblast/ECM/biomaterial interface that exhibits greater adhesion power than is seen on smoother surfaces (21). Enhanced osteoblast differentiation is also seen on electron micromachined substrates that have both micron scale and submicron scale structural elements (22,23).…”

mentioning

confidence: 99%

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

Olivares-Navarrete

Raz

Zhao

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

215

182

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Efforts to improve bone response to biomaterials have focused on ligands that bind ␣5␤1 integrins. However, antibodies to ␣5␤1 reduce osteoblast proliferation but do not affect differentiation when cells are grown on titanium (Ti). ␤1-silencing blocks the differentiation stimulus of Ti microtopography, suggesting that other ␤1 partners are important. Stably ␣2-silenced MG63 human osteoblast-like cells were used to test whether ␣2␤1 specifically mediates osteoblast response to Ti surface micron-scale structure and energy. WT and ␣2-silenced MG63 cells were cultured on tissue culture polystyrene (TCPS) and Ti disks with different surface microtopographies: machined pretreatment (PT) surfaces [mean peak to valley roughness (R a) < 0.02 m], PT surfaces that were grit-blasted and acid-etched (SLA; R a ‫؍‬ 4 m), and SLA with high surface energy (modSLA). Alkaline phosphatase (ALP), ␣2 and ␤1 mRNA, but not ␣5, ␣v, ␤3, type-I collagen, or osteocalcin, increased on SLA and modSLA at 6 days. ␣2 increased at 8 days on TCPS and PT, but remained unchanged on SLA and modSLA. ␣2-protein was reduced 70% in ␣2-siRNA cells, whereas ␣5-mRNA and protein were unaffected. ␣2-knockdown blocked surface-dependent increases in ␤1 and osteocalcin and decreases in cell number and increases in ALP and local factors typical of MG63 cells grown on SLA and modSLA [e.g., prostaglandin E 2, osteoprotegerin, latent and active TGF-␤1, and stimulatory effects of 1␣,25(OH)2D3 on these parameters]. This finding indicates that ␣2␤1 signaling is required for osteoblastic differentiation caused by Ti microstructure and surface energy, suggesting that conclusions based on cell behavior on TCPS are not predictive of behavior on other substrates or the mechanisms involved.␣-2 integrin siRNA ͉ MG63 human osteoblasts ͉ titanium surface roughness T itanium (Ti) and Ti alloys are commonly used as biomaterials because their surface properties provide a biocompatible interface with peri-implant tissues. Strategies for modifying the nature of this interface frequently involve changes to the surface, thereby affecting protein adsorption, cell-substrate interactions, and tissue development (1). A common modification has been to create micron-scale and submicron scale roughness. Preclinical and clinical studies (2-12) show that these surfaces support greater bone-to-implant contact than smooth surfaces.How surface microstructure promotes an osteogenic response is an important question, because bone-forming osteoblasts preferentially colonize bone surfaces that have been preconditioned by bone-resorbing osteoclasts (13), resulting in complex micron-scale and submicron-scale morphologies (14). In vitro experiments using model surfaces indicate that migration, growth, and colony morphology of rat bone marrow cells (15) and osteoblasts (16-18) are sensitive to microstructure. These observations suggest that structural elements can modulate the spatial organization of cells and their ECM.The topography of osteoclast resorption pits in bone can be modeled by using Ti subs...

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Topography effects of pure titanium substrates on human osteoblast long-term adhesion

Cited by 281 publications

References 38 publications

Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

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