“…Nevertheless, for the analysis of biological effects, this methodological feature is irrelevant to determining the anatomical dimensions of a primary tooth; moreover, radiation in paediatric patients should be restricted to extremely necessary cases. However, the “in vitro” methodology is more rigorous in anatomical studies, such as this and other studies [ 1 , 2 , 3 , 4 , 5 , 6 , 9 , 11 , 12 , 13 , 16 , 27 , 34 , 35 , 36 ], by allowing the fixation of the teeth during handling.…”