Torilis japonica extract, a new potential EMT suppressor agent by regulation of EGFR signaling pathways

Abstract

Abnormal metastasis of carcinoma is associated with the loss of epithelial features and the acquisition of a mesenchymal phenotype. The stimulation of cells with epidermal growth factor (EGF) resulted in morphological changes and induced epithelial-mesenchymal transition (EMT). EGF stimulation resulted in increased mobility along with upregulated actin polarization related proteins, E-cadherin regulators and the mesenchymal markers. Treatment with Torilis japonica extract (TJE) along with stimulation by EGF prevented changes in cell morphology, mobility, expression of actin polarization proteins and EMT markers. Using specific inhibitors and siEGFR, it was demonstrated that TJE suppressed EMT through EGFR inactivation and regulation of its downstream signaling pathways. We suggest that TJE is a new potential reagent for EGFR-targeted therapy and anti-abnormal metastasis in MCF-7 breast cancer.

Torilis japonica extract-generated intracellular ROS induces apoptosis by reducing the mitochondrial membrane potential via regulation of the AMPK-p38 MAPK signaling pathway in HCT116 colon cancer

“…Previously, we found that ethanol extracts from the fruit of Torilis japonica arrested abnormal metastasis through regulation of the EGFR signaling pathway in MCF-7 breast cancer cells (27). In this study, we focused on the effects of TJE on the induction of apoptosis through alterations in the mitochondrial membrane potential in colorectal cancer cells.…”

“…For this reason, there has been an increased interest in the effect of compounds extracted from natural sources on the prevention and treatment of colorectal cancer. Such compounds induce apoptosis and arrest metastasis through the regulation of intracellular signaling in cancer cells (27)(28)(29)(30)(31)(32)(33). Many researchers have identified food extracts that have been used in cancer chemotherapy experiments.…”

“…Previously, we found that a 95% ethanol extract from Torilis japonica had beneficial effects on metastasis through regulation of the EGFR signaling pathway in MCF-7 breast cancer cells (27). However, its anti-proliferative and apoptosis-inducing effects have not yet been elucidated.…”

Torilis japonica extract fraction compound, EGFR-targeted inhibition of cancer abnormal metastasis in A549 lung cancer cells

“…Therefore, to inhibit abnormal growth and metastasis of cancer cells, it is very important to find a target substance capable of inhibiting the EGFR activation. Recently, we discovered a novel substance that can inhibit the abnormal metastasis of MCF-7 breast cancer cells by targeting EGFR (17).…”

Effects of Torilis japonica Extract on DNCB-induced Contact Dermatitis in BALB/c Mice

“…The fruits of Torilis japonica (TJ) is 'Sa-Sang-Ja', which is traditional medical herbs prescribed as anti-fungal, anti-allergenic, antibacterial and sedative agent 30) . In addition, several studies have demonstrated that TJ possess anti-inflammatory 31) , anti-angiogenic 32) , anti-invasive 33) , and anti-microbial 34) effects.…”