Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages

An, Hyo‐Jin; Kim, In-Tae; Park, Hee-Juhn; Kim, Hyung-Min; Choi, Jung‐Hye; Lee, Kyung‐Tae

doi:10.1016/j.intimp.2011.01.002

Cited by 87 publications

(41 citation statements)

References 29 publications

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“…26) Activation of nuclear factor-κB (NF-κB) is a key signaling mechanism in LPS-induced COX-2 expression in A549 cells 27,28) and RAW 264.7 cells. 29) NDE is unlikely to act on such common mechanisms inducing COX-2 expression in A549 and RAW 264.7 cells. Further investigations on differences of signal transduction mechanism underlying LPSinduced COX-2 expression between A549 and RAW 264.7 cells should give clues for elucidating the mechanism of action of NDE.…”

Section: Fig 7 Effects Of Nde Fractions On Lps-induced Cox-2 Expresmentioning

confidence: 91%

Extract from <i>Nandina domestica</i> Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

Ueki

Akaishi

Okumura³

et al. 2012

Biological & Pharmaceutical Bulletin

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. To identify active constituent(s) of NDE responsible for the anti-inflammatory effect, NDE was introduced in a polyaromatic absorbent resin column and stepwise eluted to yield water fraction, 20% methanol fraction, 40% methanol fraction, 99.8% methanol fraction, and 99.5% acetone fraction. However, none of these five fractions alone inhibited LPS-induced COX-2 expression. On the other hand, exclusion of water fraction from NDE abolished the inhibitory effect of NDE on LPS-induced COX-2 expression. These results suggest that constituent(s) present in water fraction is required but not sufficient for the anti-inflammatory activity of NDE, which may result from interactions among multiple constituents.

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Section: Fig 7 Effects Of Nde Fractions On Lps-induced Cox-2 Expresmentioning

confidence: 91%

Extract from <i>Nandina domestica</i> Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

Ueki

Akaishi

Okumura³

et al. 2012

Biological & Pharmaceutical Bulletin

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“…These observations coupled with the development of the reddish color in the LiebermannBurchard test [31] (data not shown), as well as the occurrence of triterpenes in the Byrsonima genus [13,14,32]. Lu et al [33] and An et al [34] demonstrated that triterpene glycosides, isolated from two species of the genus Bupleurum, have in vitro anti-inflammatory activity via the inhibition of the release of NO and PGE 2 (prostaglandin E 2 ).…”

Section: Compounds Identificationmentioning

confidence: 93%

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

et al. 2016

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An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography-mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

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“…In the inflammatory state, activated immune cells, such as macrophages secrete large amounts of proinflammatory cytokines and nitric oxide (NO). High levels of proinflammatory cytokines and NO in chronic inflammatory states can result in various pathological conditions [9]. In macrophages, lipopolysaccharide (LPS), a well-known endotoxin, induces the production of the inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β, as well as inflammatory mediators, including NO and prostaglandin E 2 (PGE 2 ), that are synthesized by inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively [8,10].…”

Section: Introductionmentioning

confidence: 99%

Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindrica

Nugroho

Song

et al. 2015

Molecules

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Phytochemical studies on the constituents of the rhizomes of Imperata cylindrica (Gramineae) were performed using high-performance liquid chromatography (HPLC). We also aimed to search for any biologically active substance capable of inhibiting nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells, by testing four compounds isolated from this plant. Four compounds, including a new chromone, isoeugenin, along with ferulic acid, p-coumaric acid, and caffeic acid were isolated and identified by NMR spectroscopy. The structure of isoeugenin was determined as 7-hydroxy-5-methoxy-2-methylchromone by the 2D-NMR technique. Among the four compounds, isoeugenin has the lowest IC 50 value on the inhibition of NO production in LPS-activated macrophage RAW264.7 cells (IC 50 , 9.33 µg/mL). In addition, isoeugenin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines mRNA levels. Taken together, these results suggest that the anti-inflammatory activity of isoeugenin is associated with the down-regulation of iNOS, COX-2, and pro-inflammatory cytokines in RAW264.7 cells. Accordingly, our results suggest that the new chromone isoegenin should be considered a potential treatment for inflammatory disease.

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Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages

Cited by 87 publications

References 29 publications

Extract from <i>Nandina domestica</i> Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

Extract from <i>Nandina domestica</i> Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindrica

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