Torque teno sus virus 1 and 2 viral loads in faeces of porcine circovirus 2-positive pigs

Abstract

Recently, studies have suggested an association between the Torque teno sus virus (TTSuV) and the Porcine circovirus-2 (PCV2) in PCV2-associated disease cases. The aim of this study was to verify TTSuVs loads in pig faeces from PCV2-positive animals with and without diarrhea from PCVADaffected and PCV2-unvaccinated herds. A total of 80 faecal samples were collected individually from nursery and grow-finish pigs with (n = 40) or without (n = 40) diarrhea. The samples were tested for PCV2 and TTSuVs by using DNA binding dye SYBR Green quantitative polymerase chain reaction (qPCR). Torque teno sus virus k2 (TTSuVk2) load in the faeces was significantly higher in the nursery pigs with diarrhea, and these pigs also exhibited significantly higher PCV2 (P < 0.01) faecal matter loads compared to the non-diarrheic animals from the same age group. Torque teno sus virus 1 (TTSuV1) viral loads were the same regardless of age group and disease condition. There were no correlations between PCV2 and TTSuV1 or TTSuVk2 and TTSuV1 viral loads; however, a weak correlation (r = 0.23, P = 0.03) was found between TTSuVk2 and PCV2 viral loads. In conclusion, TTSuVk2 viral loads were significantly higher in the diarrheic faeces from the nursery pigs. Additionally, the higher loads of PCV2 and TTSuVk2 in the nursery-diarrheic animals revealed that diarrhea might have an important role in the spread of both viruses in herds.

Detection of torque teno sus virus infection in Indian pigs

“…Further, there is a report that the rate of detection of TTSuVs in pigs with clinical signs of the coinfecting pathogens is roughly double than that of clinically healthy pigs [10]. In addition, recent studies have reported a high rate of coinfection between TTSuVs and porcine circovirus (PCV)-associated disease including in PCV3 infection [9,11,12]. In India, there are few studies on the presence and distribution of TTVs in the human population [13][14][15]; nevertheless, the rate of TTV detection has been found to be significantly higher (92.8%) for Available at www.veterinaryworld.org/Vol.12/September-2019/17.pdf non-A-E hepatitis group [16].…”