Total antioxidant capacity in patients with varicoceles

Meucci, Elisabetta; Milardi, Domenico; Mordente, Alvaro; Martorana, Giuseppe; Giacchi, E.; Marinis, Laura De; Mancini, Antonio

doi:10.1016/s0015-0282(03)00404-7

Cited by 64 publications

(47 citation statements)

References 18 publications

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“…It was reported that surgical treatment could successfully eliminate over 90% of varicocoele, and in 60-80% of men, semen quality could be improved after varicocoelectomy (Schlesinger et al, 1994;Goldstein & Tanrikut, 2006; Practice Committee of the American Society for Reproductive M, Society for Male R & Urology, 2014). Considering the effect of varicocoelectomy on sperm DNA integrity and OS, it was reported that infertile patients with varicocoele would have a significant decrease in 8-hydroxy-2 0 -deoxyguanosine in sperm DNA and an increase in antioxidant capacity in seminal plasma after varicocoelectomy (Hendin et al, 1999;Mostafa et al, 2001;Meucci et al, 2003;Chen et al, 2008 Figure 2 The total number of the pregnant couples was 10 in the non-operative patients (SubVc, n = 4; NCVc, n = 6), and 12 in the operative AOCVc patients at 6 months. Histograms demonstrate the differences in sperm DNA fragmentation index (DFI) (A) and seminal MDA (B) levels between non-pregnant (NP) and pregnant (P) couples in the non-operative patients (SubVc and NCVc) at 6 months (6 m), and in the operative patients (AOCVc) at 3 (3 m) and 6 months (6 m).…”

Section: Discussionmentioning

confidence: 99%

A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

Steger

Yang

et al. 2016

Andrology

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SUMMARYOne of the main pathogeneses of varicocoele and infertility is oxidative stress (OS), nevertheless, the oxidative damaged DNA in infertile patients with varicocoele remains poorly clarified. The objective of this study was to comprehensively investigate whether sperm DNA damage and OS injury were related with different issues of varicocoele. According to the varicocoele practice guidelines, surgical treatment was not indicated in the infertile patients with subclinical (SubVc, n = 15) and normozoospermic clinical varicocoele (NCVc, n = 22), the infertile astheno/oligozoospermic patients with clinical varicocoele (AOCVc, n = 51) would receive microsurgerical varicocoelectomy. Normozoospermic healthy donors with proven fertility (n = 25) were recruited as controls. Thiobarbituric acid and sperm chromatin structure assay (SCSA) methods were preformed to analyze seminal lipid peroxidation product malondialdehyde (MDA) and sperm DNA fragmentation index (DFI). We found that AOCVc and NCVc, except SubVc, could significantly elevate sperm DFI and seminal MDA levels. Varicocoelectomy could substantially improve semen parameters, and reduce sperm DFI and seminal MDA levels in the AOCVc patients. However, the non-operative NCVc patients would possibly suffer a severe deterioration of semen parameters accompanied by aberrantly higher levels of sperm DFI and seminal MDA, whereas no differences occurred in the non-operative SubVc patients. Sperm DFI level in the pregnant group was much lower compared to the non-pregnant group (AOCVc, p < 0.01; NCVc, p < 0.05) with the best cutoff value of 19.73%, while no differences in seminal MDA (p > 0.05) could be observed. Finally, a strong positive correlation was found between sperm DFI and seminal MDA (Rs = 0.504, p < 0.01), and they were also closely correlated with crucial semen parameters except normal morphology. Therefore, sperm DNA damage in clinical varicocoele, but not in SubVc, might be associated with the role of seminal reactive oxygen species (ROS) in mediating such damage. Varicocoelectomy could be beneficial for reducing OS injury and sperm DFI, and males with low sperm fragmented-DNA level had more opportunities to become pregnant.

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Section: Discussionmentioning

confidence: 99%

A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

Steger

Yang

et al. 2016

Andrology

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“…Additionally, TQ pretreatment resulted in statistically significantly lower TOS levels. Previous studies have shown that antioxidant levels indicate a balance between production and utilization and that the increased TAC values could indicate an ineffective utilization of the antioxidant system in patients with varicocele and oligospermia [25] . Evidence shows that a testicular torsion of 720° for 2 h causes disruption to the seminiferous epithelium and significantly reduces testis weight and daily sperm production [26] .…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Thymoquinone in Experimental Testicular Torsion

Gökçe¹,

Oktar²,

Köç³

et al. 2010

Urol Int

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Objectives: To investigate the protective role of thymoquinone (TQ) on unilateral testicular ischemia-reperfusion (I/R) injury in mice. Materials and Methods: Experiments were performed on male C57BL/6 mice (8 weeks old, 20–25 g). The animals were divided into 3 groups including 6 mice in each group: control (sham), torsion/detorsion (TD) and TD+TQ. Mice, except the sham-operated group, were subjected to left unilateral torsion (720° rotation in the clockwise direction). The experiments were finished after sham operation time for controls, 120 min torsion and 240 min detorsion for the other groups. In the TD+TQ group 10 mg TQ was injected intraperitoneally 30 min before detorsion. Results: In the TD group total oxidative stress (TOS), oxidative stress index (OSI) and malondialdehyde (MDA) levels were higher than in the controls. TQ treatment decreased MDA, TOS and OSI values, but did not affect the total antioxidant capacity and myeloperoxidase activity in the TD+TQ group. Upon histological examination, mice in the TD group displayed moderate-to-severe disruption of the seminiferous epithelium. Treatment with TQ resulted in significantly reduced histological damage associated with I/R injury. Conclusion: Our results suggested that TQ treatment may have a protective effect on testicular I/R injury.

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“…Total Antioxidant Capacity (TAC) was evaluated, with a modification of the method developed by Rice-Evans and Miller [11], as previously described [12]. The method is based on the antioxidants inhibition of the absorbance of the radical cation ) formed by interaction between ABTS (150 μM) and ferryl myoglobin radical species, generated by activation of meta myoglobin (2.5 μM) with H 2 O 2 ( 75 μM).…”

Section: Main Outcome Measuresmentioning

confidence: 99%

“…In the Lag mode, the assay mainly measures nonprotein and nonenzymatic antioxidants that are primarily extracellular chain breaking antioxidants, such as ascorbate, urate and glutathione [12].…”

Section: Main Outcome Measuresmentioning

confidence: 99%

Effects of Intramuscular or Bioadhesive Buccal Testosterone Treatment on Antioxidant Systems in Secondary Hypogonadism

Raimondo¹

2013

J Steroids Horm Sci

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Total antioxidant capacity in patients with varicoceles

Cited by 64 publications

References 18 publications

A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

Protective Effect of Thymoquinone in Experimental Testicular Torsion

Effects of Intramuscular or Bioadhesive Buccal Testosterone Treatment on Antioxidant Systems in Secondary Hypogonadism

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