Total antioxidant capacity, lipid peroxidation, thiol group and catalase activity in patients with kidney stone

Abstract

Background: Urinary tract stones are one of the most common causes of kidney disease. There is evidence for the possible involvement of oxidative stress in the formation of kidney stones and renal cell injury. Objectives: In this study, we aimed to determine the total antioxidant capacity (TAC), malondialdehyde (MDA), serum thiol group, ceruloplasmin (CP) levels and catalase (CAT) activity in the serum of the patients with kidney stones. Materials and Methods: This study was conducted as a case-control study. A total of 31 patients (16 males and 15 females) with kidney stone(s) and a comparative normal control group including 21 (12 males and 9 females) healthy subjects were included. Results: The present study revealed that TAC level was significantly higher in the control group (P=0.004), and the mean thiol group was remarkably decreased in the patient group (P<0.001). Moreover, CAT activity decreased in the patients but it was not significant (P=0.23). On the other hand, the findings showed that the serum CP level significantly increased in the patient group (P<0.001). MDA level also increased in the patient group, although this increment was not significant (P=0.87). Conclusions: It can be concluded that the reduction of antioxidant indices in the patients with kidney stones can damage the renal tubular cells and strengthen the accumulation of crystals and the formation of kidney stones.

Synergistic effect of doxycycline and aqueous extract of irradiated khella on structure of nanobacteria isolated from kidney stones: In vitro and in vivo studies

“…In addition, the results obtained by Rostampour et al 65 showed that MDA levels were higher in the subjects with kidney stones compared to the healthy subjects, though this increase was not significant. Finally, reactive oxygen species (ROS) were known to perturb the oxidant‐antioxidant balance in kidney cells, resulting in cellular damage 67 .…”

“…Additionally, the kidneys were potentially exposed to high concentrations of oxidants and reactive electrophiles. As well, Rostampour et al 65 stated that oxidative stress caused the reduction of GSH. Likewise, Carrasco‐Valiente et al 66 reported that LPO was significantly higher in the subjects with kidney stones compared to the subjects without kidney stones.…”

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“…The assay principles were based on the reaction of catalase to decompose hydrogen dioxide. According to the kit instructions (Kiazist, Iran), samples were mixed with buffer assay (hydrogen peroxide) and the reduction of absorbance as a result of hydrogen peroxidase elimination were recorded at 240 nm ( 20 ).…”

Potential Anti-Inflammatory and Growth Inhibitory Effect of Cyrtopodion scabrum Extract on Colon Cancer; An in vivo Study

“…The level of GSH, as a main antioxidant parameter in the body, was evaluated in the sera of experimental mice, using Ellman's method with minor modification (Rostampour et al, 2017). The process was based on the formation of GS-TNB complex from DTNB (5, 5' dithiobis 2-nitrobenzoic acid) which developed yellow color because of the DTNB reduction.…”