Total antioxidant levels, gender, and age as risk factors for DNA damage in lymphocytes of the elderly

The Effect of Flavonoids in Soybean Products in Lymphocytes from IBD and Colon Cancer Patients After Treatment with Food Mutagens and Hydrogen Peroxide

“…These differences in repair capacity may either be an effect of cancer per se due to a changed lymphocyte population alongside the oncogenic process, or a higher DNA damage and slower repair among some individuals who may be more predisposed to develop cancer (Palyvoda et al, 2003). An analysis of confounding factors such as age, gender, diet, smoking habits and alcohol intake (Table 4) on the baseline level of DNA damage in lymphocytes in Study II showed a significant higher damage for subjects of >50 years of age (p < 0.01) as previously reported (King et al, 1994;Mendoza-Nunez et al, 2001). When examining the confounding effect aspects the numbers of participants were reduced yet we still found statistically significant effects for age (>50. p < 0.01) for both OTM and % tail DNA and for gender (males, p < 0.05) for % tail DNA only.…”

“…When examining the confounding effect aspects the numbers of participants were reduced yet we still found statistically significant effects for age (>50. p < 0.01) for both OTM and % tail DNA and for gender (males, p < 0.05) for % tail DNA only. An increase in DNA damage in lymphocytes has been observed among elderly individuals but was not significant (Betti et al, 1994;Mendoza-Nunez et al, 2001). We are aware our groups are not best matched for age however these were the only individuals available at the time.…”

“…DNA damage in male lymphocytes, however, was significantly higher than in lymphocytes from females for the Comet assay parameter % tail DNA but not for OTM. According to the literature, male gender constituted a risk factor for DNA damage (Collins et al, 1998a), where elderly males had more than twice the probability of having DNA damage than females (Mendoza-Nunez et al, 2001). After in vitro treatment with IQ and PhIP an increase in DNA damage in lymphocytes was observed in our study but confounding factors such as diet, smoking and drinking habits did not seem to influence this response, even though numerous environmental and lifestyle compounds can have an impact on the exposure, metabolism and cell proliferation response of HCA (Felton et al, 2004).…”

Aging-Related Oxidative Stress in Healthy Humans

“…This number increases with aging, and joined to certain pro-oxidant factors such as smoking, alcoholism, environmental contamination, ultraviolet radiation, insuffi cient hours of sleep, extenuating physical activity, inadequate nutrition, masculine gender, drug consumption, and psychological stress determine the genesis of chronic diseases during old age (Ames et al 1993;Hicks et al 1996;D'Almeida et al 1998; Mastaloudis et al 2001;Mendoza-Núñez et al 2001).…”

Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

“…Istraživanja na populacijama o spolno uvjetovanim razlikama u razinama primarnih oštećenja DNA dala su kontradiktorne rezultate (191)(192)(193)(194), a neka istraživanja nisu utvrdila značajne razlike (195,196). Neki autori našli su porast razine primarnih oštećenja DNA u ovisnosti o dobi (193,197,198), drugi ne (191,192,195,196,199).…”