Total Automation of an Antibiotic Bioassay

Burns, Donald A.; Hansen, Gary D.

doi:10.1111/j.1749-6632.1968.tb11768.x

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…Earlier publications (3,4) indicated that cultures need to be agitated in the tubes just before reading. Two methods for mixing the contents of a tube are the introduction of a slight flow of compressed air by a probe into the tube and the use of a mechanical stirrer.…”

Section: Discussionmentioning

confidence: 99%

Automated Turbidimetric Microbiological Assay Readout System▲

Rippere

Arret

1972

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

Automated Turbidimetric Microbiological Assay Readout System▲

Rippere

Arret

1972

Journal of Pharmaceutical Sciences

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“…When there is a substantial density difference, particles can be permitted to settle out in discrete systems or be made to fall through one leg of an appropriate fitting in continuous-flow systems. Filtration is the last resort, and can be done by batch with self-cleaning filters (8) or continuously by employing a moving strip of filter paper. In both cases, the addition of a flocculating agent is often helpful.…”

Section: Principlesmentioning

confidence: 99%

Automated sample preparation

Burns¹

1981

Anal. Chem.

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An improved, totally automated antibiotic bioassay machine

Burns

Williams

Hansen

1969

Biotech & Bioengineering

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SummaryA number of improvements have been made in a totally-automated antibiotic bioassay machine previously described. The new machine accepts unmeasured, untreated, opaque suspensions of fermentation beers three times faster (120 samples per hour) and supplies printed potencies sooner (in just over two hours). Whereas the original machine employed a self-cleaning filter and used disposable two milliliter beakers, this version involves a batch-dialysis scheme for effecting sample purification, and provides for automated cleaning of incubation chambers.In operation, a measured portion of thoroughly-mixed fermentation beer is automatically diluted and transferred into one side of an incubation chamber, the two halves of which are separated by a dialysis membrane. The other half i6 filled with inoculated media. During the two hour incubation at 37", dialyzable antibiotic limits growth of the inoculum in proportion to its concentration. After incubation, the turbidity of the inoculum is simultaneously read by an online computer and plotted on a strip chart recorder. The computer supplies printed potency values and sample identification on site, while the recording provides the operator with an analog record of turbidity. Fiber optics are employed in the turbidimetric readout., and an electric typewriter provides the printout.

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Total Automation of an Antibiotic Bioassay

Cited by 8 publications

References 9 publications

Automated Turbidimetric Microbiological Assay Readout System▲

Automated Turbidimetric Microbiological Assay Readout System▲

Automated sample preparation

An improved, totally automated antibiotic bioassay machine

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