2007
DOI: 10.1110/ps.072876107
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Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit

Abstract: The potassium channel accessory subunit KChIP2 associates with Kv4.2 channels in the cardiac myocyte and is involved in the regulation of the transient outward current (I to ) during the early phase of repolarization of the action potential. As a first step to biophysically probe the mechanism of KChIP2, we have chemically synthesized its minimal isoform, KChIP2d, using Boc chemistry solid phase peptide synthesis in conjunction with native chemical ligation. The synthetic KChIP2d protein is primarily alpha-hel… Show more

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Cited by 12 publications
(9 citation statements)
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“…For instance, a uniformly 13 C-and a uniformly 13 C, 15 N-labeled set would introduce 15 N-labels into a 13 C ''background'', or vice versa, or, for larger proteins, all amino acids could be perdeuterated and some could bear additional labels.…”
Section: Introductionmentioning
confidence: 99%
See 4 more Smart Citations
“…For instance, a uniformly 13 C-and a uniformly 13 C, 15 N-labeled set would introduce 15 N-labels into a 13 C ''background'', or vice versa, or, for larger proteins, all amino acids could be perdeuterated and some could bear additional labels.…”
Section: Introductionmentioning
confidence: 99%
“…Nuclear magnetic resonance (NMR) is a continuously evolving powerful tool to study the atomic-level structure, internal dynamics, partner-molecule (ligand) binding and folding of biomolecules (mostly) in aqueous solution. NMR spectroscopy is based on the magnetic properties of atomic nuclei, preferably those with ½ spin, such as 1 H, 13 C and 15 N. The most-common natural isotopes of carbon and nitrogen, 12 C and 14 N are not readily accessible for measurements. By utilizing exclusively the 1 H nuclei, which are the most abundant NMR-active nuclei in biomolecules, only the three-dimensional structures and only of relatively small proteins (up to 50 amino acid residues) can be studied routinely.…”
Section: Introductionmentioning
confidence: 99%
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