Total control of fat cells from adipogenesis to apoptosis using a xanthene analog

Tung, Ching–Hsuan; Han, Myung Shin; Qi, Jianjun

doi:10.1371/journal.pone.0179158

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…[ 36 ] Further, the overcrowded adipocytes produce cytokines and adipokines due to stress. [ 37 ] This is consistent with the observation of ceramides (Cer 38:0;O3, m/z 650.55), which are generally implicated in apoptosis. [ 38 ] The full list of lipids found in this study is in the supporting information (Tables S1 and S2, Supporting Information).…”

Section: Resultssupporting

confidence: 88%

Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

Mehta

Shaik

Prasad

et al. 2021

Adv Funct Materials

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Stem cell‐based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective treatment requires precise non‐destructive evaluation of the purity of stem cells with high sensitivity (<0.001% of the number of cells). Here, a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping‐based machine learning analysis is reported to distinguish differentiating human adipose‐derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enables identifying differentiated cells at single‐cell resolution. The label‐free HSI method is compared with the standard techniques such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. HSI is successfully used to assess the abundance of adipocytes derived from transplanted cells in a transgenic mice model. Further, Raman microscopy and multiphoton‐based metabolic imaging is performed to provide complementary information for the functional imaging of the hASCs. Finally, the HSI method is validated using matrix‐assisted laser desorption/ionization‐mass spectrometry imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label‐free identification of stem cell differentiation with high spatial and chemical resolution.

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Section: Resultssupporting

confidence: 88%

Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

Mehta

Shaik

Prasad

et al. 2021

Adv Funct Materials

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show abstract

“…We found PG 40:4 (m/z 827.578), a prominent mitochondrial phospholipids, responsible for cell growth and promoter of anaerobic metabolism [70]. Further, the overcrowded adipocytes produce cytokines and adipokines due to stress [71]. This is consistent with the observation of ceramides (Cer 38:0;O3, m/z 650.55), which are generally implicated in apoptosis [72].…”

Section: Resultssupporting

confidence: 75%

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Mehta

Shaik

Prasad

et al. 2020

Preprint

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Stem cell-based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective therapy requires precise non-destructive evaluation of the purity of stem cells with high sensitivity (< 0.001% of the number of cells). Here, we report a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping (SAM)-based machine learning analysis to distinguish differentiating human adipose derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enabled identification of differentiated cells at single cell resolution. The label-free HSI method was compared with the standard methods such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. Further, we performed Raman microscopy and multiphoton-based metabolic imaging to provide complimentary information for the functional imaging of the hASCs. Finally, the HSI method was validated using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label-free identification of stem cell differentiation with high spatial and chemical resolution. This could provide a powerful tool to assess the safety and efficacy of stem cell-based regenerative therapies.

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“…More recently, a xanthene analog [27] has been proposed to control fat cells from adipogenesis. This synthesized xanthene MI-401 was found to have two distinct effects on the regulation of the adipocyte's life cycle.…”

Section: Resultsmentioning

confidence: 99%

Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro

Fuggetta

Zonfrillo

Villivà

et al. 2019

Mediators of Inflammation

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Objective Obesity is considered a clinic condition characterized by a state of chronic low-grade inflammation. The role of macrophages and adipocytokines in adipose tissue inflammation is in growing investigation. The physiopathological mechanisms involved in inflammatory state in obesity are not fully understood though the adipocytokines seem to characterize the biochemical link between obesity and inflammation. The aim of this work is to analyze the effect of theobromine, a methylxanthine present in the cocoa, on adipogenesis and on proinflammatory cytokines evaluated in a model of fat tissue inflammation in vitro. Methods In order to mimic in vitro this inflammatory condition, we investigated the interactions between human-like macrophages U937 and human adipocyte cell lines SGBS. The effect of theobromine on in vitro cell growth, cell cycle, adipogenesis, and cytokines release in the supernatants has been evaluated. Results Theobromine significantly inhibits the differentiation of preadipocytes in mature adipocytes and reduces the levels of proinflammatory cytokines as MCP-1 and IL-1β in the supernatants obtained by the mature adipocytes and macrophages interaction. Conclusion Theobromine reduces adipogenesis and proinflammatory cytokines; these data suggest its potential therapeutic effect for treating obesity by control of macrophages infiltration in adipose tissue and inflammation.

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Total control of fat cells from adipogenesis to apoptosis using a xanthene analog

Cited by 8 publications

References 48 publications

Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro

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