Total energy expenditure assessed by salivary doubly labelled water analysis and its relevance for short‐term energy balance in humans

Guidotti, Stefano; Verstappen-Dumoulin, B. M. A. A.; Jansen, H.W.B.; Aerts-Bijma, Anita; Vliet, André A. van; Scheurink, Antonius; Meijer, H. A. J.; Dijk, Gertjan van

doi:10.1002/rcm.7412

Cited by 4 publications

(6 citation statements)

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“…Daily energy expenditure was calculated using the doublylabeled water method as previously described by our group (Guidotti et al, 2013(Guidotti et al, , 2016. Determination of 2 H/ 1 H and 18 O/ 16 O ratios in saliva and blood samples were performed at the ESRIG Center for Isotope Research, University of Groningen.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

A low TSH profile predicts olanzapine-induced weight gain and relief by adjunctive topiramate in healthy male volunteers

Evers

Vliet²,

Vugt³

et al. 2016

Psychoneuroendocrinology

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Section: Laboratory Investigationsmentioning

confidence: 99%

A low TSH profile predicts olanzapine-induced weight gain and relief by adjunctive topiramate in healthy male volunteers

Evers

Vliet²,

Vugt³

et al. 2016

Psychoneuroendocrinology

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“…The body dilution space (N) can be measured based on the plateau method (equation ( 6)) or on the intercept method (equation ( 7)), and the total body water is calculated using equation (9). In figure 4 we compare the body water percentage (water / mass) results calculated by TLW with those by EchoMRI.…”

Section: Total Body Water (Tbw) and Dilution Spacementioning

confidence: 99%

“…The 2 H and 18 O abundances of body fluids are measured from the initial to the final time points, and then the isotope elimination rates can be determined. The difference between these two turnover rates is then proportional to the CO2 production (rCO2), which can be further converted to energy expenditure if the composition of the food intake is known 1,4,[7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

“…For a long time, IRMS has been the technique for DLW water analysis. As IRMS functions with pure gases, pre-treatment for the (water) samples is necessary, preceded by distillation if the body fluid is blood 2,4,9,28 . Optical spectroscopic measurement of water vapour has become a reliable alternative, initially thanks to the pioneering activities in our laboratory 14,29,30 .…”

Section: Introductionmentioning

confidence: 99%

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Validity of Triply Labelled Water Analysis for Energy Expenditure Measurements in Mice

Wang

Kong

Dijk

et al. 2021

Preprint

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The Doubly Labelled Water (DLW) method is widely used to determine energy expenditure. In this work, we demonstrate the addition of the third stable isotope, 17O, to turn it into Triply Labelled Water (TLW), using the three isotopes measurement of optical spectrometry. We performed TLW (2H, 18O and17O) measurements for the analysis of the CO2 production (rCO2) of mice on different diets for the first time. Triply highly enriched water was injected into mice, and the isotope enrichments of the distilled blood samples of one initial and two finals were measured by an Off-Axis Integrated Cavity Output Spectroscopy instrument. We evaluated the impact of different calculation protocols and the values of evaporative water loss fraction. We found that the dilution space and turnover rates of 17O and 18O were equal for the same mice group, and that values of rCO2 calculated based on 18O-2H, or on 17O-2H agreed very well. This increases the reliability and redundancy of the measurements and it lowers the uncertainty in the calculated rCO2 to 3% when taking the average of two DLW methods. However, the TLW method overestimated the rCO2 compared to the indirect calorimetry measurements that we also performed, much more for the mice on a high-fat diet than for low-fat. We hypothesize an extra loss or exchange mechanism with a high fractionation for 2H to explain this difference.

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“…The 2 H and 18 O abundances of body fluids are measured from the initial to the final time points, and then the isotope elimination rates can be determined. The difference between these two turnover rates is then proportional to the CO 2 production (r CO2 ), which can be further converted to energy expenditure if the composition of the food intake is known 1 , 4 , 7 – 9 .…”

Section: Introductionmentioning

confidence: 99%

First use of triply labelled water analysis for energy expenditure measurements in mice

Wang

Kong

Dijk

et al. 2022

Sci Rep

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The doubly labelled water (DLW) method is widely used to determine energy expenditure. In this work, we demonstrate the addition of the third stable isotope, 17O, to turn it into triply labelled water (TLW), using the three isotopes measurement of optical spectrometry. We performed TLW (2H, 18O and17O) measurements for the analysis of the CO2 production (rCO2) of mice on different diets for the first time. Triply highly enriched water was injected into mice, and the isotope enrichments of the distilled blood samples of one initial and two finals were measured by an off-axis integrated cavity output spectroscopy instrument. We evaluated the impact of different calculation protocols and the values of evaporative water loss fraction. We found that the dilution space and turnover rates of 17O and 18O were equal for the same mice group, and that values of rCO2 calculated based on 18O–2H, or on 17O–2H agreed very well. This increases the reliability and redundancy of the measurements and it lowers the uncertainty in the calculated rCO2 to 3% when taking the average of two DLW methods. However, the TLW method overestimated the rCO2 compared to the indirect calorimetry measurements that we also performed, much more for the mice on a high-fat diet than for low-fat. We hypothesize an extra loss or exchange mechanism with a high fractionation for 2H to explain this difference.

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Total energy expenditure assessed by salivary doubly labelled water analysis and its relevance for short‐term energy balance in humans

Abstract: The DLW method in humans can be reliably applied using saliva as sampling fluid. TEI-TEE as well as AEE contributes significantly to changes in fat mass over an 8-day period.

Cited by 4 publications

References 31 publications

A low TSH profile predicts olanzapine-induced weight gain and relief by adjunctive topiramate in healthy male volunteers

A low TSH profile predicts olanzapine-induced weight gain and relief by adjunctive topiramate in healthy male volunteers

Validity of Triply Labelled Water Analysis for Energy Expenditure Measurements in Mice

First use of triply labelled water analysis for energy expenditure measurements in mice

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