Total evidence phylogeny and the evolution of adult bioluminescence in fireflies (Coleoptera: Lampyridae)

Martin, Gavin J.; Branham, Marc; Whiting, Michael F.; Bybee, Seth

doi:10.1016/j.ympev.2016.12.017

Cited by 79 publications

(84 citation statements)

References 33 publications

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“…B) with high support values of bootstraps (NJ: 97%; ML: 86%; BI: 1). Although Lamprigera was originally placed in Lampyrinae in traditional taxonomy , it was recently suggested to be placed in the Lampyridae Incerte sedis . Our results were also supported by morphological and molecular data .…”

Section: Resultssupporting

confidence: 83%

“…Although Lamprigera was originally placed in Lampyrinae in traditional taxonomy (4), it was recently suggested to be placed in the Lampyridae Incerte sedis (15). Our results were also supported by morphological and molecular data (13)(14)(15). It is noted that incongruence for the phylogenetic position of Lamprigera was observed in NJ and ML/BI trees.…”

Section: Phylogenetic Analyses Of Luciferases From Lamprigera Fireflisupporting

confidence: 80%

“…Lamprigera is a small genus in Lampyridae and includes only 17 species which is distributed in the Asian countries from Himalaya-Karakoran-Tibet region to Southeast Asia (4,12). Though originally placed in the subfamily Lampyrinae, this genus showed an unresolved taxonomic position at the subfamily level from morphological traits or/and from molecular evidence (13)(14)(15). Based on our experiences, the insects of this genus can be often observed near villages or even in the garden of city center.…”

Section: Introductionmentioning

confidence: 92%

“…Effect of temperature on Lamprigera yunnana (filled circle) and Pyrocoelia pygidialis (filled square) luciferase activity. The activity at six varied temperature (15,20,30,37, 50 and 55°C) were detected, and the standard deviation as an error bar.…”

Section: Luciferase Activity and Emission Spectrum Of Recombinant Lammentioning

confidence: 99%

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Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana

Liu

Zhang

Hou

et al. 2019

Photochem & Photobiology

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Lamprigera (Lampyridae) is a small genus with only 17 species distributing in Asian countries. Its larviform females and alate males can produce continuously strong yellow‐green light at night. However, no luciferase gene was reported for this genus and its subfamily‐level phylogenetic position still remains uncertain. Here, we cloned the luciferase gene from one Chinese species, Lamprigera yunnana, by reverse transcription‐polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE). This luciferase includes 551 deduced amino acids (AA) with the sequence identity of 71.8–76.8%, 67.5–70.7%, 68.4–75.3%, 77.8% and 59.5% to those from Lampyrinae, Luciolinae, Ototretinae, Cyphonocerinae and Photurinae, respectively. Phylogenetic analyses of deduced AA of luciferases suggest that Lamprigera locates outside Lampyrinae, in which it was originally placed in traditional taxonomy. The luciferase was produced in vitro as recombinant protein, and its biochemical properties were characterized. It possesses significant luminescence activity at pH 7.8, and its KM for D‐luciferin and ATP are 61 μm and 122 μm, respectively. It shows the highest activity at 37°C and is completely inactivated at 55°C. It is pH‐sensitive with the maximum emission spectrum of 566 nm at pH 7.8. Our data provide new insights into Lamprigera luciferase and its phylogenetic position.

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Section: Resultssupporting

confidence: 83%

Section: Phylogenetic Analyses Of Luciferases From Lamprigera Fireflisupporting

confidence: 80%

Section: Introductionmentioning

confidence: 92%

Section: Luciferase Activity and Emission Spectrum Of Recombinant Lammentioning

confidence: 99%

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Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana

Liu

Zhang

Hou

et al. 2019

Photochem & Photobiology

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“…Molecular analyses have suggested that the origin of Lampyridae was dated back to late Jurassic (156) or mid-Cretaceous periods (202). Luciolinae and Lampyrinae was diverged at the basal position of the Lampyridae (203) and the fossil of the Luciolinae firefly dated at Cretaceous period was discovered in Burmese amber (204,205). Taken together, the divergence of Luciola and Lampyridae is dated back at least 100 Mya.…”

Section: Luciferase Genetics Overviewmentioning

confidence: 99%

Firefly genomes illuminate parallel origins of bioluminescence in beetles

Fallon

Lower

Chang

et al. 2017

Preprint

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SummaryFireflies are among the best-studied of the bioluminescent organisms. Despite longterm interest in the biochemistry, neurobiology, and evolution of firefly flash signals and the widespread biotechnological applications of firefly luciferase, only a limited set of genes related to this complex trait have been described. To investigate the genetic basis of firefly bioluminescence, we generated a high-quality reference genome for the Big Dipper firefly Photinus pyralis, from which the first laboratory luciferase was cloned, using long-read (PacBio), short-read (Illumina), and Hi-C sequencing technologies. To facilitate comparative genomics, we also generated short-read genome assemblies for a Japanese firefly Aquatica lateralis and a bioluminescent click beetle, Ignelater luminosus. Analyses of these genomic datasets supports at least two independent gains of luminescence in beetles, and provides new insights into the evolution of beetle bioluminescence and chemical defenses that likely co-evolved over their 100 million years of evolution.

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