Total fish meal replacement with canola protein isolate in diets fed to rainbow trout (Oncorhynchus mykissW.)

Abstract

The potential of canola protein isolate (CPI) as fish meal alternative in diets for rainbow trout was evaluated. Apparent digestibility coefficients (ADCs) for protein from a fish meal-based reference diet (89.2 ± 1.1%) and CPI (84.6 ± 1.8%) were determined by indirect marker method in a digestibility experiment. ADC of dietary dry matter was slightly lower for the reference diet (62.5 ± 4.7%), but not significantly different to the CPI test diet (65.9 ± 3.1%). In a subsequent growth trial, 20 fish (initial weight 31.5 ± 0.5 g) were stocked into each of 15 experimental tanks of a freshwater flow-through system. Fish were organized in triplicate groups and received experimental diets with 0%, 25%, 50%, 75% and 100% of fish meal replaced with CPI on the basis of digestible protein (designated as Control, I25, I50, I75, I100 respectively). At the end of a 70-day feeding period, growth performance, feed intake and feed conversion ratio of treatment groups receiving diets I25, I50 or I100 were similar to the control group, while fish fed diet I75 showed significantly higher weight gain caused by improved feed conversion. The tested CPI was therefore identified to be a highly valuable fish meal alternative, not negatively affecting diet palatability, feed intake and feed efficiencies.

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

“…Rather, the RPI 100 fed group recorded the highest feed intake, though not differ from other fed groups, indicating that diets palatability and acceptability were not affected. Our findings are in consonant with Slawski et al (2013) and Kumar et al (2012), who observed no significant difference in the feed intake of rainbow trout and common carp fed canola and jatropha protein isolates, respectively. Sharma et al (2014) reported that feeding RKM diets containing a high level of cyanide (>39 mg kg −1 ) to rohu led to a reduction in protein utilization and digestion processes, which resulted in poor growth performance.…”

“…The variation in their results may be due to the levels of ANFs, nonstarch polysaccharide, and or species differences. Literature have shown that varying level of antimetabolites, indigestible carbohydrates, amino acid imbalances and other complexes may contribute to growth depression often observed when plant protein ingredients are fed at higher inclusion levels to fish, rather than pinpointing one single factor as the primary reason for the adverse effects noticed (Mambrini et al, 1999;Francis and Becker, 2001;Schulz et al, 2007;Slawski et al, 2013;Shamna et al, 2015). However, in the present study, no significant variation was observed in the growth performance of the fish fed RPI in replacement for SPI.…”

“…The protein content of RPI was similar to canola protein isolate (Mwachireya et al, 1999), but more than those reported by for rapeseed protein isolate (Nagel et al, 2012), pea protein isolate (Schulz et al, 2007), canola protein isolate (Slawski et al, 2013) and jatropha protein isolate . Isolating the proteins from rubber kernel meal resulted in a significant increase in the protein content and reduction in the antinutritional factors as reported by other researchers in different studies (Devappa and Swamylingappa 2008;Saetae and Suntornsuk, 2010;Nagel et al, 2012;Kumar et al, 2012;Slawski et al, 2013). The higher in vitro digestibility of RPI recorded in this study may be due to the significant reduction in the soluble and indigestible carbohydrates, antinutritional factors, and more accessibility of amino acid peptide bonds by the digestive enzymes as reported by Devappa and Swamylingappa (2008).…”

“…The cyanide content in RPI (27 mg kg −1 ) was less than the value reported for detoxified rubber kernel meal (60.1 mg kg −1 ) fed to L. rohita fingerling (Sharma et al, 2014). The protein content of RPI was similar to canola protein isolate (Mwachireya et al, 1999), but more than those reported by for rapeseed protein isolate (Nagel et al, 2012), pea protein isolate (Schulz et al, 2007), canola protein isolate (Slawski et al, 2013) and jatropha protein isolate . Isolating the proteins from rubber kernel meal resulted in a significant increase in the protein content and reduction in the antinutritional factors as reported by other researchers in different studies (Devappa and Swamylingappa 2008;Saetae and Suntornsuk, 2010;Nagel et al, 2012;Kumar et al, 2012;Slawski et al, 2013).…”

“…Since rubber kernel meal contain less protein, a large amount of indigestible material and anti-nutrients, there is need to convert them into a better useful products by isolating the protein for optimum utilization. Several studies were conducted recently to explore the inclusion level of various plant protein isolates in fish such as soybean protein isolate in Acipenser schrenckii , rapeseed (Brassica napus) protein isolate in juvenile Psetta maxima (Nagel et al, 2012), canola (Brassica campestris) protein isolate in Oncorhynchus mykiss (Slawski et al, 2013), jatropha protein isolate in Cyprinus carpio Latif et al, 2015), and pea protein isolate in juvenile Oreochromis niloticus (Schulz et al, 2007). The results from these findings were encouraging, and to the best of our knowledge, no work on protein isolates from rubber seeds has been reported in fish and livestock.…”