Total flavonoids extracted from <i>Nervilia Fordii</i> function in polycystic ovary syndrome through IL-6 mediated JAK2/STAT3 signaling pathway

Zhou, Yumin; Lv, Liang; Liu, Qinghua; Song, Junxian

doi:10.1042/bsr20181380

Cited by 25 publications

(20 citation statements)

References 30 publications

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“…Rutin, a avonoid, ameliorates hyperandrogenism, acyclicity and infertility to improve PCOS phenotypes [9]. Flavonoids from Nervilia Fordii can down-regulate FSH serum level, up-regulate of LH and T serum levels and recover estrous cycle, exerting a therapeutic e ciency for PCOS treatment [26]. Our results are in line with these previous ndings that herbal bioactive avonoids can exert a protective effect on PCOS.…”

Section: Discussionsupporting

confidence: 89%

Rhamnocitrin ameliorates ovarian fibrosis via PPARγ/TGF-β1/Smad2/3 pathway to repair ovarian function in polycystic ovary syndrome rats

Zhou

Dong

et al. 2021

Preprint

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Background: Polycystic ovary syndrome (PCOS) is one of the major endocrine disorders in women, characterized by androgen excess, chronic anovulation and ovarian fibrosis. Rhamnocitrin is an herbal bioactive flavonoid that has anti-inflammation and antioxidant effects. We intended to investigate the impacts of Rhamnocitrin on PCOS-induced ovarian fibrosis and its underlying mechanisms.Methods:Dehydroepiandrosterone (DHEA) induced-PCOS rats were treated with Rhamnocitrin. HE staining was performed to detect ovarian histological features. Ovarian fibrosis was evaluated by Sirius Red and Masson staining. Vaginal smear was examined to exhibit estrus cycle stage and vaginal cornification. The serum hormone levels of FSH, LH, E2 and T were measured with ELISA. The related mRNAs and proteins of fibrosis factors and PPARγ/TGF-β1/Smad2/3 signaling were detected by RT-qPCR and western blot. The weights of rat bodies and ovaries were recorded. PPARγ inhibitor T0070907 and its agonist GW1929 were employed for the mechanistic investigation.Results:The corpus luteum and follicles were increased and irregular estrous cycle was restored after Rhamnocitrin treatment in PCOS rats. Rhamnocitrin inhibited ovarian fibrosis and down-regulated the expressions of fibrotic factors. Rhamnocitrin reduced the increased levels of LH, E2, and T, and elevated the decreased FSH level in PCOS rats. Besides, Rhamnocitrin elevated the down-regulated PPARγ, and suppressed the up-regulated TGF-β1 and p-Smad2/3 expressions induced by PCOS. These effects of Rhamnocitrin on PCOS rats could be antagonized by T0070907, whereas GW1929 markedly mimics the functions of Rhamnocitrin.Conclusions:Rhamnocitrin ameliorates ovarian fibrosis in PCOS rats through regulation PPARγ/TGF-β1/Smad2/3 pathway, suggesting it can be a potentially effective therapeutic candidate for PCOS treatment.

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Section: Discussionsupporting

confidence: 89%

Rhamnocitrin ameliorates ovarian fibrosis via PPARγ/TGF-β1/Smad2/3 pathway to repair ovarian function in polycystic ovary syndrome rats

Zhou

Dong

et al. 2021

Preprint

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“…According to previous reports, flavonoids were demonstrated to show evident bioactivities in DOR treatments. For instance, total flavonoids regulated serum levels of FSH, luteinizing hormone (LH), and testosterone (T) in polycystic ovary syndrome rats by inhibiting the JAK2/STAT3 pathway (Zhou et al, 2019). Flavonoids also inhibited oxidative stress of endometrial cells to improve pregnancy outcome (Estany et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

et al. 2020

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Backgrounds: Diminished ovarian reserve (DOR) contributes significantly to female infertility. Bushen Cuyun Recipe (BCR, Tradename Yueliang Yin), a product marketed in China, has shown effects in the treatment of female infertility in clinical practices of traditional Chinese medicine (TCM). In this study, we aimed to investigate the chemical compositions of BCR and its efficacy based on scientific evidence and pharmacological mechanisms in DOR treatments. Methods: The chemical compositions of BCR were determined by the UHPLC-LTQ-Orbitrap MS method. DOR was induced in a rat model by intraperitoneal injection of cyclophosphamide (CTX) 90 mg/kg once. After the CTX treatment for 14 days, rats were intragastrically administrated deionized water, dehydroepiandrosterone (DHEA), or BCR in low, middle, and high doses for 30 days. Ovarian index, ovarian morphology, follicle number, and anti-Müllerian hormone (AMH) in serum were determined to assess the effects of BCR. To investigate possible action mechanisms, network pharmacological analysis was used to predict possible pathways in the effects of BCR on female infertility. In experimental studies, the contents of hormones in the hypothalamic-pituitary-ovarian axis (HPOA, including estradiol (E 2), follicle-stimulating hormone (FSH), and gonadotropinreleasing hormone (GnRH)) and pyroptosis-related proteins, including gasdermin D (GSDMD), caspase-1, and interleukin-18 (IL-18), in ovarian were detected by ELISA, immunofluorescence and Western blot. Results: Chemical studies revealed a total 84 components in BCR, which included 43 flavonoids, 13 triterpenoids, 11 phenolic acids, 8 alkaloids, 1 coumarin, 1 anthraquinone, and 7 other components. After treatments with BCR, the ovarian morphology, ovarian index, estrous cycle, growing follicles and corpus luteum from last ovulation, and serum AMH in DOR rats were significantly improved. Network pharmacological analysis suggested that the NOD-like receptor signaling pathway ranked No. 1 among the mechanisms by which BCR affects female infertility. Experimental results demonstrated

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“…Flavonoid baicalin (isolated from Scutellaria baicalensis ) has been shown to decrease levels of testosterone, LH, progesterone and estradiol, and several proinflammatory cytokines (IL-1β, IL-18 and TNFα) and normalise IR in PCOS-induced rats [ 96 ]. Similarly, flavonoids recovered from Nervilia Fordii decrease the levels of IL-6 and hormones LH, testosterone and insulin and increase the serum level of FSH [ 97 ]. Plant-delivered alkaloid berberine has been shown to reduce levels of IR and testosterone in PCOS-induced rats.…”

Section: Pcos Treatments: Classical and Modern Mitochondria–oriented Approachesmentioning

confidence: 99%

Mitochondrial Dysfunction and Chronic Inflammation in Polycystic Ovary Syndrome

Dabravolski

Nikiforov

Eid

et al. 2021

IJMS

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Polycystic ovarian syndrome (PCOS) is the most common endocrine–metabolic disorder affecting a vast population worldwide; it is linked with anovulation, mitochondrial dysfunctions and hormonal disbalance. Mutations in mtDNA have been identified in PCOS patients and likely play an important role in PCOS aetiology and pathogenesis; however, their causative role in PCOS development requires further investigation. As a low-grade chronic inflammation disease, PCOS patients have permanently elevated levels of inflammatory markers (TNF-α, CRP, IL-6, IL-8, IL-18). In this review, we summarise recent data regarding the role of mtDNA mutations and mitochondrial malfunctions in PCOS pathogenesis. Furthermore, we discuss recent papers dedicated to the identification of novel biomarkers for early PCOS diagnosis. Finally, traditional and new mitochondria-targeted treatments are discussed. This review intends to emphasise the key role of oxidative stress and chronic inflammation in PCOS pathogenesis; however, the exact molecular mechanism is mostly unknown and requires further investigation.

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Total flavonoids extracted from Nervilia Fordii function in polycystic ovary syndrome through IL-6 mediated JAK2/STAT3 signaling pathway

Cited by 25 publications

References 30 publications

Rhamnocitrin ameliorates ovarian fibrosis via PPARγ/TGF-β1/Smad2/3 pathway to repair ovarian function in polycystic ovary syndrome rats

Rhamnocitrin ameliorates ovarian fibrosis via PPARγ/TGF-β1/Smad2/3 pathway to repair ovarian function in polycystic ovary syndrome rats

Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats

Mitochondrial Dysfunction and Chronic Inflammation in Polycystic Ovary Syndrome

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