Total Flavonoids of Bidens pilosa Ameliorates Bone Destruction in Collagen-Induced Arthritis

Hong, Mengqin; Fan, Xuesen; Liang, Shengxiang; Wang, Xiang; Chen, Liting; Yang, Yuzhong; Deng, Yan

doi:10.1055/a-1352-5124

Cited by 9 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flavonoids and phenols are important components of diets and medications due to their inherent health-promoting properties, which include anti-oxidative, anti-inflammatory, anti-mutagenic, anti-carcinogenic, antimicrobial, and larvicidal properties [59]. Bidens pilosa has been reported to be used in the treatment of various diseases, including hyperglycemia [8], malaria [9], tumours [10], and inflammation [12], which could be linked to the high possession of flavonoids and phenols. The antioxidant activities of the ethanol extracts are higher compared to the water extract.…”

Section: Cytotoxicity Using Rtcamentioning

confidence: 99%

“…It belongs to the Asteraceae family, has yellow flowers, and grows to a height of 30-100 cm. B. pilosa is used for [8], malaria [9], tumours [10], microbial infections [11], rheumatoid arthritis, and inflammation [12]. Heavy metals such as arsenic and cadmium have been reported to bioaccumulate in B. pilosa, which suggests that the plant has the potential for the phytoremediation of heavy metals [4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

et al. 2023

View full text Add to dashboard Cite

Purpose Bidens pilosa L. is traditionally used as a flavouring agent in foods, in the treatment of diseases, in agriculture as a biopesticide and herbicide, and in the phytoremediation of soils contaminated with heavy metals. The vast range of uses of B. pilosa for a variety of purposes is questionable, hence motivating the objectives of this study, which are to assess the cytotoxicity, health benefits, and/or risks of B. pilosa using chemical-based evidence. Methods A real-time cell analysis (xCELLigence system), ultra-high-performance liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer, high-performance liquid chromatography with a diode array detector, and chemical-based spectrophotometric methods were adopted in the study. Results High concentrations of the ethanol extracts exhibited cytotoxic activity on HepG2 (cancerous), and Vero (non-cancerous) cell lines, whereas the water extracts promoted cell proliferation at selected concentrations. The chemical profiling enabled the separation as well as characterisation of 137 phytochemicals. These were mainly phenolic acids, flavonoids, fatty acids, coumarins, and furanocoumarins. There was no toxic compound identified. Conclusion The ethanol extracts are generally more potent and exhibit stronger antioxidant activity and cytotoxicity, probably due to the presence of more flavonoids and phenolic acids, validating the uses of B. pilosa and its relevance as a source of functional phytochemicals.

show abstract

Section: Cytotoxicity Using Rtcamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Both the control and induction groups were intraperitoneally (IP)-injected with 0.5ml of normal saline (NaCl). Moreover, the third group of rats were treated with 1mg/kg of DTX every other day, based on our preliminary experiment; and the fourth group IP injected with 1mg/kg/week of MTX (24) ; and the fifth group was the combination group, which received half doses of both MTX and DTX.…”

Section: Animalsmentioning

confidence: 99%

Effect of Docetaxel on Matrix Metalloproteinase 1 Expression in Freund’s Adjuvant Induced Arthritis

Alghulami,

Ghaith A. Jasim,

Suzan Yousif Jasim

2024

IJPS

View full text Add to dashboard Cite

Rheumatoid arthritis is an autoimmune disorder that affects the synovial joints. It lead to inflammatory changes occur in synovial tissue, cartilage, bone, and, less frequently, extra-articular structures. Docetaxel, is an anti-neoplastic drug. Methotrexate is a treatment for early-stage rheumatoid arthritis but have 30% failure in treatment within the first year due to resistance or side effects. Matrix metalloproteinase 1 is the proteinase primarily in charge of the irreversible degradation of cartilage, bone, and tendons in the joints, which are induced by cytokines. This study aims to evaluate the effects of docetaxel and methotrexate on Matrix metalloproteinase 1 in the structure of the induced arthritis joints. Methods: To induce the disease, complete Freund's adjuvant is injected subcutaneously into rats. From the 40 male Wister rats, five groups of eight were formed. The control group is composed of non-diseased rats. The second group is induced by complete Freund's adjuvant and 0.5 ml of normal saline was administered intraperitoneally to both the control and induction groups. After being induced by complete Freund's adjuvant, the third group is administered 1 mg/kg/ on alternate day of Docetaxel based on a preliminary experiment. The fourth group is induced and given 1 mg/kg/week of Methotrexate intraperitoneally. The fifth group receives a half dose of Methotrexate and Docetaxel simultaneously. Serum level of Anti Cyclical citrullinated Peptide Antibody, tissue homogenate level of tumor necrosis factor-alpha, knee joint circumference and Immunohistochemical of Matrix metalloproteinase 1 measurements were applied. Results: Significant decreased in the Serum level of Anti-Cyclic citrullinated Peptide Antibody, tissue homogenate level of tumor necrosis factor-alpha & Knee joint circumference in the Docetaxel group. significant lowering in the expression of Matrix metalloproteinase 1 immunoreactivities in Docetaxel and Methotrexate groups with almost absent spots of positive reaction with the matrix of synovial tissue, hyaline cartilage & articular surface. Conclusion: This study showed that Docetaxel may have anti-arthritic effect through their significant lowering expression of Matrix metalloproteinase 1 immunoreactivities.

show abstract

“…The third group is induced by CFA and given 1 mg/kg/on alternative day DTX based on preliminary experiment. Fourth group was induced, then given 1mg/kg/week MTX intraperitoneally [28] . The fifth group was the combination group that's induced then treated with MTX and DTX in half doses On days 0 and 10, animals in the induction, DTX, MTX, and DTX+MTX groups received (1.2 and 0.4) ml of CFA in their tail bases.…”

Section: Animalsmentioning

confidence: 99%

Histopathological evaluation of docetaxel effects in treatment of rheumatoid arthritis induced in rat model

Omar Mustafa Alghulami,

Ghaith A. Jasim,

Suzan Yousif Jasim**

2023

AJPS

View full text Add to dashboard Cite

Rheumatoid arthritis is an immune-mediated condition that affects synovial joints. Synovial tissue, cartilage, bone, and less frequently extra-articular structures which in turn experience inflammatory changes. Paclitaxel's semi-synthetic equivalent, docetaxel, is an anti-neoplastic drug. Methotrexate is a treatment for early RA and may have a mildly negative impact on peptidyl arginine deiminase type 4 fluorescence test. However, 30% of patients fail to complete treatment within the first year due to resistance or side effects. The synovial membrane of Rheumatoid arthritis patient infiltrated with macrophages and neutrophils that express peptidyl arginine deiminase type 4 which their effect in rheumatoid arthritis pathogenesis lies in the generation of citrullinated neoepitopes that are Anti cyclic citrullinated peptide antibodies-targeted. The purpose of this study: was to assess the anti-inflammatory effects of docetaxel and methotrexate on the joint structure. Methods: Five groups of eight rats were formed from the 40 male Wister rats. Complete Freund’s adjuvant was injected subcutaneously into rats to induce the disease. The first group is control group which was the only group consists of (healthy untreated) rats. Second group was received complete Freund’s adjuvant. 0.5ml of ordinary saline was intraperitoneally administered to both the control and induction groups. Based on a preliminary experiment, the third group was given intraperitoneally 1 mg/kg/on alternative day docetaxel. The fourth group was given intraperitoneally 1 mg/kg/week of Methotrexate. Fifth group was given a half dose of both Methotrexate and docetaxel concurrently. Arthritis index was measured and Knee joint was histopathological examined. Results: significant Arthritis Index decrease in docetaxel group (p≤0.05). Significant lowering Histometric scoring (p≤0.05) in docetaxel, and Methotrexate group (cellular hyperplasia, formation of granulation tissue, infiltration of leukocytes, destroying of cartilage and intensity of erosion & Articular cartilage thickness) level in rats induced arthritis. Conclusion: This study showed that docetaxel may have anti-arthritic effects through their significant lowering Histometric scoring(p≤0.05).

show abstract

Total Flavonoids of Bidens pilosa Ameliorates Bone Destruction in Collagen-Induced Arthritis

Cited by 9 publications

References 49 publications

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

Effect of Docetaxel on Matrix Metalloproteinase 1 Expression in Freund’s Adjuvant Induced Arthritis

Histopathological evaluation of docetaxel effects in treatment of rheumatoid arthritis induced in rat model

Contact Info

Product

Resources

About