Total glucosides of paeony improves the immunomodulatory capacity of MSCs partially via the miR-124/STAT3 pathway in oral lichen planus

Zhao, Zhongfang; Han, Ying; Zhang, Zhihui; Li, Wenwen; Ji, Xiaoli; Liu, Xiaodan; Jin, Jianqiu; Xu, Shumao; Cui, Hongmei; Cheng, Zhe; Wang, Qian; Wang, Xing; Guo, Xiang; Wang, Yixiang; Liu, Hongwei

doi:10.1016/j.biopha.2018.05.076

Cited by 25 publications

(20 citation statements)

References 41 publications

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“…STAT3 can mediate the onset of inflammatory reactions via IL-6/JAK pathway [ 45 ]. In addition, STATs can also modulate inflammatory/immune responses by regulating COX-2 and IDO, becoming intermediate bridges linking tumors and inflammatory responses [ 46 ]. In our study, the activation of JAK2 and STAT3 were significantly inhibited by miR-218 overexpression.…”

Section: Discussionmentioning

confidence: 99%

MiR-218 produces anti-tumor effects on cervical cancer cells in vitro

Zhu¹,

Tu²,

Liang³

et al. 2018

World J Surg Onc

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BackgroundAs indoleamine-2,3-dioxygenase 1 (IDO1) is critical in tumor immune escape, we determined to study the regulatory mechanism of miR-218 on IDO1 in cervical cancer.MethodsReal-time PCR (RT-qPCR) was carried out to measure the expression of miR-218. RT-qPCR and Western blot were performed to detect the expression of IDO1 in cervical cancer. Dual-luciferase reporter assay was used to determine the binding of miR-218 on the IDO1 3′UTR. Cell viability, apoptosis, and related factors were determined using cell counting kit-8 (CCK-8), Annexin-V/PI (propidium) assay, enzyme-linked immunosorbnent assay (ELISA), RT-qPCR, and Western blot assays after miR-218 mimics has been transfected to HeLa cervical cancer cells.ResultsMiR-218 was downregulated in cervical cancer. The expression of miR-218 was negatively correlated with IDO1 in cervical cancer tissues and cells. IDO1 is a direct target of miR-218. MiR-218 overexpression was found to inhibit cell viability and promoted apoptosis via activating the expression of Cleaved-Caspase-3 and to inhibit the expression of Survivin, immune factors (TGF-β, VEGF, IL-6, PGE2, COX-2), and JAK2/STAT3 pathway.ConclusionMiR-218 inhibits immune escape of cervical cancer cells by direct downregulating IDO1.

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Section: Discussionmentioning

confidence: 99%

MiR-218 produces anti-tumor effects on cervical cancer cells in vitro

Zhu¹,

Tu²,

Liang³

et al. 2018

World J Surg Onc

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“…Clinically, the majority of OLP patients experience oral discomfort, even in the process of spicy or acidic foods intake 4 . Only a small group of OLP patients (about 15%) accompany with cutaneous lesions and around 0.4-5% patients with OLP have a risk of developing oral carcinoma 5 . In histopathology, the main characteristics of diseased tissues of OLP include T lymphocytes-infiltrated band in lamina propria and cytoid bodies in the epithelial layer, which represent the presence of inflammatory response and apoptosis, respectively 1,6,7 .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-26a/b have protective roles in oral lichen planus

Gao

Wang

et al. 2020

Cell Death Dis

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Oral lichen planus (OLP) is a kind of oral epithelial disorder featured with keratinocyte apoptosis and inflammatory reaction. The pathogenesis of OLP remains an enigma. Herein, we showed that the levels of miR-26a/b were robustly down-regulated in oral mucosal biopsies, serum and saliva in OLP patients compared with healthy control. Moreover, we found the binding sites of vitamin D receptor (VDR) in the promoter regions of miR-26a/b genes and proved that the induction of miR-26a/b was VDR dependent. The reduction of miR-26a/b expression was also detected in the oral epithelium of vitamin D deficient or VDR knockout mice. miR-26a/b inhibitors enhanced apoptosis and Type 1T helper (Th1) cells-related cytokines production in oral keratinocytes, whereas miR-26a/b mimics were protective. Mechanistically, we analyzed miRNA target genes and confirmed that miR-26a/b blocked apoptosis by directly targeting Protein Kinase C δ (PKCδ) which promotes cellular apoptotic processes. Meanwhile, miR-26a/b suppressed Th1-related cytokines secretion through targeting cluster of the differentiation 38 (CD38). In accordant with miR-26a/b decreases, PKCδ and CD38 levels were highly elevated in OLP patients' samples. Taken together, our present investigations suggest that vitamin D/VDR-induced miR-26a/b take protective functions in OLP via both inhibiting apoptosis and impeding inflammatory response in oral keratinocytes.

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“…Studies have shown that some kinds of autoimmune diseases, such as primary Sjogren's syndrome and inflammatory bowel disease, can be treated by regulating regulatory T cells/T helper cell 17 cells (Chen et al, 2019;. TGP treatment can increase the expression of TGF-b and IL-10, up-regulate peripheral Treg cells and the transcription factor Foxp3 in Treg cells (Zhao et al, 2012;Zhao et al, 2018) and inhibit the levels of Th17-related cytokines like IL-6 and IL-17 (Lin et al, 2012;Lin et al, 2017). IL-1b is also down-regulated by TGP treatment (Zhang et al, 2014).…”

Section: The Pharmacological Actions Of Rpamentioning

confidence: 99%

“…TGP treatment can increase the expression of TGF-β and IL-10, up-regulate peripheral Treg cells and the transcription factor Foxp3 in Treg cells ( Zhao et al., 2012 ; Zhao et al., 2018 ) and inhibit the levels of Th17-related cytokines like IL-6 and IL-17 ( Lin et al., 2012 ; Lin et al., 2017 ). IL-1β is also down-regulated by TGP treatment ( Zhang et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Efficacy, Chemical Constituents, and Pharmacological Actions of Radix Paeoniae Rubra and Radix Paeoniae Alba

et al. 2020

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are the different characteristic forms of Paeonia lactiflora Pall. They are widely used as traditional Chinese medicines in clinical practices. This study analyzes the development history, efficacy, chemical compositions, and pharmacological effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, and explores the causes of the similarities and differences of these two amalgams. It provides a basis for the clinical application of these two Chinese medicinal materials, and lays a foundation for further study of the pharmacological effects and the quality identification of Paeonia lactiflora Pall as it applies to traditional Chinese medicine.

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Total glucosides of paeony improves the immunomodulatory capacity of MSCs partially via the miR-124/STAT3 pathway in oral lichen planus

Cited by 25 publications

References 41 publications

MiR-218 produces anti-tumor effects on cervical cancer cells in vitro

MiR-218 produces anti-tumor effects on cervical cancer cells in vitro

MicroRNA-26a/b have protective roles in oral lichen planus

Efficacy, Chemical Constituents, and Pharmacological Actions of Radix Paeoniae Rubra and Radix Paeoniae Alba

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