Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice

Huang, Qiling; Ma, Xiaomeng; Zhu, Dong liang; Chen, Li; Jiang, Ying; Zhou, Linli; Lei, Cen; Chen, Xiaohong

doi:10.1016/j.jneuroim.2015.05.009

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…NC, normal control group; TGP, total glycosides of paeony; SASP, sulfasalazine; Th17, effector CD4 + T helper 17; Treg, regulatory CD4 + T cells; TNBS, trinitrobenzene sulfonic acid-induced group; TNBS+SASP, TNBS-induced rats treated with TNBS-induced rats treated with SASP; IL, interleukin; TNF-α, tumor necrosis factor-α; ROR-γt, retinoic acid related orphan receptor-γt; TNF-β, transforming growth factor-β; Foxp3, forkhead boxp3. autoimmune encephalomyelitis, collagen induced arthritis and TNBS-induced colitis (26,30,33,36), it remains to be elucidated how TGP exerts its therapeutic effects. Previous studies have revealed that the potential mechanism of TGP in TNBS-induced colitis may be associated with the adjustment of Th1/Th2 cytokine polarization (37,47).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies revealed that TGP may inhibit Th1/Th17 immune response by downregulating the expression of transcriptional factor T-box expressed in T cells (T-bet) and RORgt. Meanwhile, TGP may increase the CD4 + CD25 + Treg differentiation by activating the transcription factor Foxp3 (30,(33)(34)(35)(36). A previous study revealed that TGP effectively attenuated inflammation in 2,4,6-trinitrobezence sulfonic acid (TNBS)/ethanol-induced colitis in rats (37).…”

Section: Total Glucosides Of Paeony Ameliorates Tnbs-induced Colitis mentioning

confidence: 99%

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Total glucosides of paeony ameliorates TNBS-induced colitis by modulating differentiation of Th17/Treg cells and the secretion of cytokines

Hai-hua

Zhang

Jiang

et al. 2017

Molecular Medicine Reports

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The imbalance between effector CD4+ T helper 17 (Th17) and regulatory CD4+ T cells (Treg) cells and their associated cytokines, have been associated with the pathogenesis of inflammatory bowel disease (IBD). Total glycosides of paeony (TGP) is an alternative immunomodulatory agent that is widely used for the treatment of autoimmune diseases. The present study aimed to evaluate the modulatory effect of TGP in a rat model of colitis induced by 2,4,6‑trinitrobenzene sulfonic acid (TNBS). TGP was administered intragastrically 24 h after the TNBS intrarectal instillation for 7 days. TGP treatment ameliorated the clinical status and reversed the histopathologic severity of acute TNBS colitis. Furthermore, TGP inhibited the levels of Th17‑associated cytokines interleukin (IL)‑17, IL‑6, tumor necrosis factor‑α, whereas the expression levels of Treg‑associated cytokines IL‑10, transforming growth factor‑β in the plasma, colon, spleen and mesenteric lymph nodes (MLN). Additionally, TGP reduced the percentage of Th17 cells; however, the proportion of Treg cells in the spleen and MLN was increased. The present study also observed a suppression of Th17‑associated transcription factor, termed retinoid‑related orphan receptor‑γt (ROR‑γt). However, expression of the Treg‑associated transcription factor forkhead boxp3 was increased in the TGP treatment group. Therefore, the present findings suggest that TGP has a regulatory role in modulating the balance of Th17 and Treg cells to ameliorate the TNBS‑induced colitis and support the strategy of using TGP to treat IBD.

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Section: Discussionmentioning

confidence: 99%

Section: Total Glucosides Of Paeony Ameliorates Tnbs-induced Colitis mentioning

confidence: 99%

Total glucosides of paeony ameliorates TNBS-induced colitis by modulating differentiation of Th17/Treg cells and the secretion of cytokines

Hai-hua

Zhang

Jiang

et al. 2017

Molecular Medicine Reports

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“…To determine the effects of GL on the initiation and progression of EAE, mice were treated using three different strategies with three different concentrations of GL (10, 25, and 50 mg/kg) as shown in Figure 1 A. Similar treatment strategies have been reported by our and other research groups ( 20 , 30 , 31 ). Strategy I injection occurred from days −1 to day 11, which we refer to as early GL treatment; strategy II injection was from days 12 to 22 as the midterm-late GL treatment; strategy III injection was from days 15 to 23 as the late GL treatment (Figure 1 A).…”

Section: Resultsmentioning

confidence: 93%

Glycyrrhizin Protects Mice Against Experimental Autoimmune Encephalomyelitis by Inhibiting High-Mobility Group Box 1 (HMGB1) Expression and Neuronal HMGB1 Release

et al. 2018

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The inflammatory mediator high-mobility group box 1 (HMGB1) plays a critical role in the pathogenesis of human multiple sclerosis (MS) and mouse experimental autoimmune encephalomyelitis (EAE). Glycyrrhizin (GL), a glycoconjugated triterpene extracted from licorice root, has the ability to inhibit the functions of HMGB1; however, GL’s function against EAE has not been thoroughly characterized to date. To determine the benefit of GL as a modulator of neuroinflammation, we used an in vivo study to examine GL’s effect on EAE along with primary cultured cortical neurons to study the GL effect on HMGB1 release. Treatment of EAE mice with GL from onset to the peak stage of disease resulted in marked attenuation of EAE severity, reduced inflammatory cell infiltration and demyelination, decreased tumor necrosis factor-alpha (TNF-α), IFN-γ, IL-17A, IL-6, and transforming growth factor-beta 1, and increased IL-4 both in serum and spinal cord homogenate. Moreover, HMGB1 levels in different body fluids were reduced, accompanied by a decrease in neuronal damage, activated astrocytes and microglia, as well as HMGB1-positive astrocytes and microglia. GL significantly reversed HMGB1 release into the medium induced by TNF-α stimulation in primary cultured cortical neurons. Taken together, the results indicate that GL has a strong neuroprotective effect on EAE mice by reducing HMGB1 expression and release and thus can be used to treat central nervous system inflammatory diseases, such as MS.

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“…Total glucosides of peony, extracted from the root of Paeonia lactiflora Pall, has been broadly used to treat autoimmune diseases like RA, multiple sclerosis, Sjögren syndromeand systemic lupus erythematosus . Paeoniflorin (PF) is the most abundant bioactive substance of total glucosides of peony and exerts various pharmacological effects including anti‐inflammatory and immune‐regulation activities.…”

Section: Introductionmentioning

confidence: 99%

Paeoniflorin ameliorates collagen‐induced arthritis via suppressing nuclear factor‐κB signalling pathway in osteoclast differentiation

Cai

Zhang

et al. 2018

Immunology

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Paeoniflorin (PF), extracted from the root of Paeonia lactiflora Pall, exhibits anti-inflammatory properties in several autoimmune diseases. Osteoclast, the only somatic cell with bone resorbing capacity, was the direct cause of bone destruction in rheumatoid arthritis (RA) and its mouse model, collagen-induced arthritis (CIA). The objective of this study was to estimate the effect of PF on CIA mice, and explore the mechanism of PF in bone destruction. We demonstrated that PF treatment significantly ameliorated CIA through inflammatory response inhibition and bone destruction suppression. Furthermore, PF treatment markedly decreased osteoclast number through the altered RANKL/RANK/OPG ratio and inflammatory cytokines profile. Consistently, we found that osteoclast differentiation was significantly inhibited by PF through down-regulation of nuclear factor-κB activation in vitro. Moreover, we found that PF suppressed nuclear factor-κB activation by decreasing its translocation to the nucleus in osteoclast precursor cells. Taken together, our new findings provide insights into a novel function of PF in osteoclastogenesis and demonstrate that PF would be a new therapeutic modality as a natural agent for RA treatment and other autoimmune conditions with bone erosion.

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Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice

Cited by 9 publications

References 52 publications

Total glucosides of paeony ameliorates TNBS-induced colitis by modulating differentiation of Th17/Treg cells and the secretion of cytokines

Total glucosides of paeony ameliorates TNBS-induced colitis by modulating differentiation of Th17/Treg cells and the secretion of cytokines

Glycyrrhizin Protects Mice Against Experimental Autoimmune Encephalomyelitis by Inhibiting High-Mobility Group Box 1 (HMGB1) Expression and Neuronal HMGB1 Release

Paeoniflorin ameliorates collagen‐induced arthritis via suppressing nuclear factor‐κB signalling pathway in osteoclast differentiation

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