Total IgE and allergen-specific IgE and IgG antibody levels in sera of atopic dermatitis affected and non-affected Labrador- and Golden retrievers

Lauber, Béatrice; Molitor, Vivianne; Meury, Sabrina; Doherr, Marcus G.; Favrot, Claude; Tengvall, Katarina; Bergvall, Kerstin; Leeb, Tosso; Roosje, Petra; Marti, Eliane

doi:10.1016/j.vetimm.2012.05.018

Cited by 44 publications

(67 citation statements)

References 35 publications

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“…These findings lead to still unanswered questions about the specificity of the commercial antibodies used in canine IgG subclass detection and differences in translation of IgG subclass responses between humans and dog. The claimed specificities of the commercial antibodies used in the present study were based on the results of previous studies …”

Section: Discussionmentioning

confidence: 99%

“…A further study reported a high level of IgG1 against D. farinae in atopic dogs which increased after immununotheraphy . Due to a lack of availability of commercial anti‐dog IgG subclass antibodies, only IgG1 and IgG2 have been studied …”

Section: Introductionmentioning

confidence: 99%

“…10 Due to a lack of availability of commercial anti-dog IgG subclass antibodies, only IgG1 and IgG2 have been studied. 11,12 The aim of this study was to investigate levels of allergen-specific IgE, IgG1 and IgG2 directed against M. pachydermatis and S. pseudintermedius, with total IgG levels, then correlate them with lesion severity in cAD as defined by the Canine Atopic Dermatitis Lesion Index (CADLI). 13…”

Section: Introductionmentioning

confidence: 99%

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Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Khantavee

Chanthick

Sookrung

et al. 2019

Veterinary Dermatology

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Background Elevated immunoglobulin E (IgE) levels to Malassezia or Staphylococcus species in human atopic dermatitis are related to the skin severity index; a similar association has not been reported in atopic dogs. Objectives To investigate serum levels of allergen‐specific IgE, total specific IgG and IgG subclasses (IgG1 and IgG2) for M. pachydermatis and S. pseudintermedius, and to correlate them with the severity of dermatitis in dogs. Animals Serum samples were collected from dogs categorized by age and disease status. Groups 1 and 2: <3‐year‐old healthy (n = 9) and atopic dogs (n = 9), respectively; and groups 3 and 4: ≥3‐year‐old healthy (n = 11) and atopic dogs (n = 14), respectively. Methods and materials Antibody levels were measured by ELISA. The Canine Atopic Dermatitis Lesion Index (CADLI) was analyzed in relation to antibody levels. Results Specific IgE and total IgG against M. pachydermatis and S. pseudintermedius were significantly increased in atopic dogs of all ages. Although differences between atopic and healthy dogs, with regard to specific IgG1 and IgG2 levels to each microbe, varied in significance within age groups. No significant relationships were found between the CADLI and any specific immunoglobulin levels for both microbe types. Conclusions and clinical importance In dog skin, microbes may act as allergens triggering inflammatory responses via IgE‐ and IgG‐dependent pathway(s). The affinity of the IgG subclass produced may vary according to antigen type. Specific IgE levels may be related to clinical disease in dogs and not to skin lesion severity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Khantavee

Chanthick

Sookrung

et al. 2019

Veterinary Dermatology

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“…1,8 Canine atopic-like dermatitis (ALD) has been defined as an inflammatory and pruritic skin disease with clinical features identical to those seen in canine AD, in which an IgE response to environmental or other allergens cannot be documented. 9,10 Because total serum IgE levels have a poor correlation with the atopic status in dogs, [11][12][13] this parameter cannot be used to distinguish AD and ALD in dogs. Thus, in dogs, the differentiation has been made on the basis of the results of intradermal (IDT) and serum allergen-specific IgE tests (AST).…”

Section: Introductionmentioning

confidence: 99%

Comparison of demographic data, disease severity and response to treatment, between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study

Botoni

Torres

Koch

et al. 2018

Veterinary Dermatology

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Background Limited information is available describing the features of canine atopic‐like dermatitis (ALD) compared with atopic dermatitis (AD). Objectives To compare demographic data, disease severity and response to therapy between ALD and AD dogs. Animals Two hundred and fifty‐three atopic dogs with intradermal and serum allergen‐specific IgE test results were selected retrospectively. Methods and materials Dogs were enrolled into the ALD group if both IgE tests were negative and into the AD group if at least one test was positive. Demographic data, pruritus level and number of body sites affected before and during therapy, in addition to maintenance therapy protocols, were compared between groups. Results There were 216 (85.38%) dogs in the AD group and 37 (14.62%) in the ALD group. The soft‐coated wheaten terrier, American Staffordshire terrier, English bulldog and Labrador retriever were over‐represented in the AD group. No significant differences between the groups were noted regarding the other demographic variables evaluated. There were no differences in the mean pruritus scores and number of affected body sites at the first visit or during treatment. Furthermore, no significant differences between the groups were noted for the maintenance treatment scores and reduction of pruritus level and number of body sites affected during treatment. Conclusions and clinical significance The soft‐coated wheaten terrier, American Staffordshire terrier, English bulldog and Labrador retriever were over‐represented in the AD group. No significant differences in the other demographic data and clinical features were noted between dogs with ALD and AD in the present study.

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“…There are numerous publications demonstrating allergen-specific IgE in dogs and we would like to share the following data to remind our colleagues of the importance of education on the appropriate use of these tests. [3][4][5][6][7][8] In the process of conducting an unrelated study, we had the opportunity to receive allergen-specific IgE enzyme-linked immunosorbent assay data on sera from healthy dogs with no clinical signs or history consistent with AD. Twenty dogs of various ages and breeds were represented ( Table 1).…”

mentioning

confidence: 99%

Allergen‐specific IgE in nonatopic dogs

Layne

DeBoer

2018

Veterinary Dermatology

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Total IgE and allergen-specific IgE and IgG antibody levels in sera of atopic dermatitis affected and non-affected Labrador- and Golden retrievers

Cited by 44 publications

References 35 publications

Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Comparison of demographic data, disease severity and response to treatment, between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study

Allergen‐specific IgE in nonatopic dogs

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