Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping

Kim, Yong Tae; Lee, Dohwan; Heo, Hyun Young; Sim, Jeong Eun; Woo, Kwang Man; Kim, Do Hyun; Im, Sung Gap; Seo, Tae Seok

doi:10.1016/j.bios.2015.11.079

Cited by 33 publications

(9 citation statements)

References 35 publications

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“…However, it is a tedious and time-consuming process and involves a bulky gel bed setup; thus, it is not commonly used in clinical NA testing. 128 Since the 1990s, integration and parallelization of NA analyses on capillary electrophoresis-based microfluidic systems have been widely investigated, which enables efficient on-chip RNA/DNA testing; 148 however, these systems are generally complicated when both precise flow control and optical detection are needed, 149 hampering its usability in practical applications. In summary, optical detection methods are generally suitable for use on integrated diagnostic systems for NA-based virus testing, and can provide merits such as high testing throughput, satisfactory sensitivity and specificity, and good cost efficiency.…”

Section: Optical Detectionmentioning

confidence: 99%

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Qin

Peng

Baravik

et al. 2020

Matter

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The pandemic of coronavirus disease 2019 (COVID-19) highlights the importance of rapid and sensitive diagnostics of viral infection that enables the efficient tracing of cases and the implementation of public health measures for disease containment. The immediate actions from both academia and industry have led to the development of many COVID-19 diagnostic systems that have secured fast-track regulatory approvals and have been serving our healthcare frontlines since the early stage of the pandemic. On diagnostic technologies, many of these clinically validated systems have significantly benefited from the recent advances in micro-and nanotechnologies in terms of platform design, analytical method, and system integration and miniaturization. The continued development of new diagnostic platforms integrating micro-and nanocomponents will address some of the shortcomings we have witnessed in the existing COVID-19 diagnostic systems. This Perspective reviews the previous and ongoing research efforts on developing integrated microand nanosystems for nucleic acid-based virus detection, and highlights promising technologies that could provide better solutions for the diagnosis of COVID-19 and other viral infectious diseases. With the summary and outlook of this rapidly evolving research field, we hope to inspire more research and development activities to better prepare our society for future public health crises.

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Section: Optical Detectionmentioning

confidence: 99%

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Qin

Peng

Baravik

et al. 2020

Matter

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“…Some examples include commercial microfluidic arrays (e.g. Fluidigm®) ( Von Thaden et al, 2017 ), microfluidic integration of sample preparation steps and/or amplification and detection steps, either based on allele-specific hybridization strategies for SNP genotyping ( Zec et al, 2018 ), or combined with microscale capillary electrophoresis (µCE) for SSR markers (Y. T. Kim et al, 2016 ). Other strategies include integration of microfluidic DNA amplification with microarrays ( Choi et al, 2012 ), primer extension on microbeads ( Chang et al, 2017 ) or implementation of HRM analysis ( Velez et al, 2017 ) at microscale.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization

Carvalho

Yadav

Garrido‐Maestu

et al. 2021

Food Chemistry: Molecular Sciences

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“…By taking full advantage of microfluidics, many researchers have made intensive efforts to conduct entire procedures of nucleic acid extraction and amplification, such as PCR and loop-mediated isothermal amplification (LAMP), on a single microfluidic device. For example, Kim et al used silicon materials and a slidable chip with 70 mm-diameter silica beads to extract genomic DNA from whole human blood [19]. A microfluidic device for integrated DNA extraction and loop-mediated isothermal amplification using glass powder has been developed to detect the epidermal growth factor receptor (EGFR) L858R point mutation in NCI-HI1975 cells [20].…”

Section: Introductionmentioning

confidence: 99%

A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

Zhu

Cui

et al. 2019

Micromachines

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Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.

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Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping

Cited by 33 publications

References 35 publications

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization

A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

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