Total internal reflection focal molography (TIR-M)

Blickenstorfer, Yves; Borghi, Linda; Reichmuth, Andreas M.; Fattinger, Christof; Vörös, János; Frutiger, Andreas

doi:10.1016/j.snb.2021.130746

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…Alexa Fluor 647 antibody labeling kit was from Thermo Fischer (Waltham, MA, USA). The silicon grooved structure Within the biosensing scenario, the fabrication of protein nanostructures points towards exploiting new light-matter phenomena to transduce biorecognition events [22][23][24][25]. The increasing incidence of chronic and inflammatory diseases such as allergies supports the development of this kind of nanobiosensors [26].…”

Section: Methodsmentioning

confidence: 99%

Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy

et al. 2023

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This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and β-lactoglobulin are created and assessed, reaching limits of detection in the range of 30–45 ng·mL−1 of unlabeled antibodies by diffractive biosensing.

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Section: Methodsmentioning

confidence: 99%

Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy

et al. 2023

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“…146 In another approach called focal molography bioreceptors are patterned according to a submicron structure (mologram) that diffracts light into a focal spot whose intensity depends on the magnitude of biorecognition events. [147][148][149][150][151][152][153] Also, previous works of our group developed diffractive immunoassays to quantify low-molecular organic compounds and exploited the compact disk technology to create arrays of biogratings to sense antibodies. 154,155 The strategy typically employed to fabricate biogratings is µCP due to its simplicity and great performance to pattern biological species.…”

Section: Introductionmentioning

confidence: 99%