PurposeThis study aimed to compare long‐term clinical and radiographic outcomes and survival rates between navigation‐assisted (NAV) total knee arthroplasty (TKA) and conventional (CON) TKA using a mobile‐bearing insert.MethodsFrom May 2008 to December 2009, 45 and 63 mobile‐bearing TKA patients were enroled in the CON‐ and NAV‐TKA groups with 146.8 months follow‐up, respectively. Clinical outcomes (Western Ontario and McMaster University Osteoarthritis Index and Knee Society Scores), radiographic outcomes (hip‐knee‐ankle [HKA], lateral distal femoral, medial proximal tibial, γ, and δ angles), and survivorship were compared between both groups.ResultsThe number of HKA angle outliers (more than 3 degrees or less than −3 degree) was significantly lower in the NAV‐TKA group (24.4% vs. 9.5%, p = 0.036) than in the CON‐TKA group. However, long‐term clinical outcomes were similar between both groups. The cumulative survival rate (best‐case scenario) was 98.3% in the CON‐TKA group and 97.5% in the NAV‐TKA group, with no significant difference between the groups (p = 0.883).ConclusionLong‐term clinical outcomes and survival rates were similar between the two groups despite fewer outliers of postoperative lower‐limb alignment in the NAV‐TKA group. Excellent survival rates were observed in both groups using mobile‐bearing inserts.Level of EvidenceLevel IV, case series.