TOTAL PHENOL AND ANTIOXIDANT ACTIVITY OF BROWN SEAWEED EXTRACT (Sargassum sp.)

Abstract

Nirwana Beach is one of the tourist destinations in Padang West Sumatra, but almost every day littered by brown seaweed that washed up along the coast. Very unfortunate, the people around still consider this waste and do not know the usefulness and benefits. Brown seaweed has been widely studied. The content consist of alginate, fukosantin and secondary metabolites namely fucoidan. Fucoidan has been made into a supplement as antioxidant, anticoagulant and antithrombotic, antiviral, anticancer, antidiabetic, immunomodulating, antiinflammatory, antilipidemic and antifertilization. The purpose of the study was to determine the antioxidant activity of polyphenol extract from brown seaweed. The brown seaweed was extracted using aquades by heating and without heating, then the extract was evaporated with a rotary evaporator up to a volume of 50 ml extract and then analyzed the total phenol and its antioxidant activity test. The results showed extraction with heating had higher total phenol content than without heating. The total phenol of brown seaweed extract with heating is 669.33 mg galic acid/g, without heating 352.5 mg of gallic acid/g and on seaweed 538.5 mg gallic acid/g. The antioxidant activity of brown seaweed extract is very strong ie 4 ug/ml with heating and 16.6 ug/ml without heating.

Extraction of Antioxidant Compounds from Sargassum sp. Using Water and Ultrasound Assisted Extraction Method as A Derivation of Green Chemistry Principles

“…In another study regarding the total phenol yield of Sargassum sp. values obtained with maceration method and water solvent gave higher total phenol yield, namely 669.33 mg GAE/g with heating for 20 minutes and 352.5 mg GAE/g without heating for 24 hours [23]. Study of Sargassum muticum using the maceration method with water solvent also gave a high total phenol yield of 275.8 ± 4.98 5 mg GAE/g.…”