Total Phenolic Content And Total Flavonoid Content In Moringa Oleifera Seed

Abstract

Moringa oleifera is one of the most popular plants in South Asian. It is known as miracle tree because of every parts of the plant including roots, leaves, pods flowers, and seeds containing high nutritional value and medicinal benefits. M. oleifera seed's oil extracted contains high antioxidants properties and become as a valuable sources of protein, vitamins, beta carotene, amino acids, and various phenolic compounds. Extraction of oil and determination of antioxidants in the oil could give a great potential for commercialization especially in pharmaceutical industries due to its pharmacological properties such as antiflammatory, antihypertensive, antiepileptic, antioxidant, antibacterial and antifungal. The aim of this study were to extract the M. oleifera seeds at different extraction time and ratio of seed to solvent and determine the amount of total phenolic content (TPC) and total flavonoid content (TFC) in the methanol extract. The extraction process was carried out using Soxhlet extraction with methanol as a solvent for different ratio of seed to solvent (1:10, 1:5 and 3:10) and extraction time (2, 3, 4, 5 and 6 hours). The highest extraction yield was found at 36.84% for the seed to solvent ratio (1:10) with the extraction time of 5 hours. The highest percentages of TPC were 2027.07 (mg GAE/g of extract) at 3 hours of extraction time and seed to solvent ratio (1:10). However, the TFC values in M. oleifera seeds were 99.72 (mg QE/g of extract weight) at 5 hours of extraction time and seed to solvent ratio (1:10). The high values of TPC and TFC in methanol extract of M. oleifera seed showing it a good source of natural antioxidant and have a great potential for commercialization in food products and pharmaceutical industries.

Black-box modelling, bi-objective optimization and ASPEN batch simulation of phenolic compound extraction from Nauclea latifolia root

“…It was noticed that the lowest TPC yield was obtained at process conditions of temperature 55 °C, ratio of 0.015 mg/ml and extraction time 3.5 h, as depicted in Table 2 respectively. The TPC results obtained from this study is comparable to previous works ( Ghafar et al., 2017 ; Kakouri et al., 2019 ). TPC experimental data were fitted into regression model in RSM and the model correlation parameters for TPC and independent variables are R 2 (0.9932), adjusted R 2 (0.932) and CV (5.76%) as shown in Table 2 .…”

Protective effect of chlorogenic acid on the focal cerebral ischemia reperfusion rat models

“…Heat-clearing and toxicity-removing medicine focuses on reducing inflammatory response, activating the self-protection mechanism of brain cell, and reducing the secondary reaction of cerebral ischemia. The application of “Activating blood to resolve stasis” combined with “Clearing away heat and removing toxin” methods for prevention and treatment of cerebral ischemic reperfusion injury allows two theories of traditional Chinese medicine and modern medicine to find the best combination point in clinical practice ( Cheng et al, 2012 , Ghafar et al, 2017 ) Preliminary study ( Cao et al, 2016 , Fang et al, 2016a , Fang et al, 2016b , Miao et al, 2013 ) finds traditional Chinese medicines with heat-clearing and toxicity-removing and blood-activating and stasis-resolving effects, such as Turmeric, rabdosia rubescens, Maodongqing, trumpet creeper, beggarticks and motherwort, all of which have good effect on resisting cerebral ischemia injury.…”

The effect of different proportions of astragaloside and curcumin on DM model of mice

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