Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L.

Abstract

Brassica oleracea var. acephala and Ornithogalum umbellatum are two plant species which belong Brassicaceae and Liliaceae, respectively. Both of them are used as food and medicinally. O. umbellatum is used as anticancer, diuretic anti anti emetic. B. oleracea var. acephala is used for gastritis, gastric and duodenal ulcers and preventing some cancer forms such as stomach cancer, breast cancer and lung cancer. The aim of this research was to search antioxidant, antibacterial and antifungal activities and FT-IR analyses of ethanol extracts of stem and flower parts of B. oleracea var. acephala and O. umbellatum. Extracts were obtained by Soxhlet extraction. Ethanol extracts of B. oleracea var. acephala showed higher antibacterial activity than ethanol extracts of O. umbellatum. The most effective antifungal extract was ethanol extract of flower part of O. umbellatum against the fungi Candida. tropicalis and Candida. parapsilosis. Antioxidant activity of the extracts were also studied. The highest total phenolic and flavonoid contents were found in flower part of B. oleracea var. acephala as 50.7?0.007 ?g GAE/mL and stem part of O. umbellatum as 81.37?0.006 ?g CE/mL, respectively. BHT which used as standard antioxidant had higher total antioxidant activity than tested ethanol extracts. DPPH activity of the extracts increased in the following order: Flower part of B. oleracea var. acephala> Stem part of O. umbellatum> Flower part of O. umbellatum> Stem part of B. oleracea var. acephala. These results which obtained from this study demonstrate that B. oleracea var. acephala and O. umbellatum species might be utilized as an alternative to synthetic antioxidans and antimicrobials.

The value of bioactive compounds of cruciferous vegetables (Brassica) as antimicrobials and antioxidants: A review

“…With a powerful antioxidant activity of 88.43 µg/g ascorbic acid equivalents. In another study by Aydin (2020), antioxidant, antibacterial activity (against Salmonella enterica, Enterococcus faecalis, Enterobacter aerogenes, and Proteus vulgaris ), and antifungal (against Candida albicans, Candida tropicalis, and Saccharomyces cerevisiae ) were found in kale extracts, obtained with ethanol as a solvent by means of the soxhlet methodology. Highlighting a greater antimicrobial activity against fungi than against bacteria.…”

Investigation of Phenolic Composition, Antioxidant Capacity, and Antidiabetic Effect of Ornithogalum lanceolatum L.: An in vitro Study

“…It was reported that DPPH IC50 values of aerial parts and bulb of Ornithogalum sintenisii at flowering stage was found to be 368±15 and 669±25 µg/mL, respectively (Ebrahimzadeh et al, 2010). However, DPPH % inhibition of O. umbellatum stem and flower parts for concentration of 200 µg/mL were determined 24.92±0.012 % and 15.31±0.002 %, respectively (Aydın, 2020). In a recent study, methanol and water extracts of bulb of Ornithogalum narbonense were calculated as 12.60±0.30 and 4.02±0.55 mg TE/g extract, respectively for DPPH (Zengin et al, 2015).…”