Total phenolic content, cyclooxygenases, ?-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

Abstract

The aim of the present study was to investigate total phenolic content and biological effects of methanol extracts from Cornus sanguinea L. leaves (LME) and fruits (FME). Total phenolic contents, COX-1/COX-2, αglucosidase, AChE, tyrosinase inhibitory and DPPH radical scavenging effects of both extracts were investigated by using spectrophotometric methods. The total phenolic contents of LME and FME were determined as 191.14 ± 4.84 and 31.51 ± 2.68 mg GAE/g dry weight, respectively. LME inhibited COX-1 enzyme 70.71 ± 1.88% and 79.38 ± 0.92% at 50 and 100 µg/mL. LME had higher COX-1 and COX-2 inhibitory effects than that of FME. LME inhibited αglucosidase stronger than positive control, acarbose. On the other hand, both extracts showed lower AChE inhibition actions compared to positive control, galantamine. Moreover, LME had higher tyrosinase inhibitory effect than FME. Both extracts scavenged DPPH radical in a concentration-dependent manner. Also, LME had stronger scavenging effect than that of FME. To our knowledge, current work is the first report on tyrosinase, AChE, as well as COX-1 inhibitory properties of C. sanguinea. These results suggested that LME of C. sanguinea have a promising potential for the treatment of several disorders but further studies are needed to support the this assumption.

Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of Cornus macrophylla Bioss (Bark)

“…In a COX-2 inhibitory assay, at 100 µg/mL, leaves extract of Cornus sanguinea displayed significant inhibition as compared with celecoxib. The IC 50 value of leaves extract was determined as 11.39 ± 2.39 µg/mL on COX-2 [24]. Our research plant showed that crude extracts have IC 50 values of 130.02µg/mL while Ethyl acetate fractions possess IC 50 values of 93.35 µg/mL compared with a standard drug, celecoxib, which had an IC 50 value of 21.58 µg/mL.…”

Вегетативне розмноження рослин роду дерен (Cornus L.): сучасний стан та перспективи використання

“…Підвищення результативності вегетативного розмноження рослин виду Сornus L., можливе за рахунок оптимізації процесів культивування та застосування сучасних стимуляторів росту з добором концентрацій розчинів [2].…”

Synthesis and antioxidant activity of benzo[a]pyrano[2,3-c] phenazine derivative compound via one-pot multicomponent reaction

“…Determination of antioxidant activity was carried out using the dpph assay method [31,32]. Concentration series of sample solution (5; 10; 50; 100; 300 ppm) in methanol solvent and DPPH solution with a concentration of 100 µM.…”