Total Phenolics and Antioxidant Potentials of Rotula aquatica Lour

Abstract

Rotula aquatica Lour is a small branched shrub that grows characteristically among rocks and trailing over gravel in stream beds. It belongs to the family Boraginaceae and widely distributed throughout India. It is commonly called as Pasanabheda. The present study was aimed to evaluate the total phenolics and in vitro antioxidant activity in root and leaf extracts of Rotula aquatica Lour. The dried roots and leaves were subjected to soxhlet extraction utilizing chloroform and methanol followed by preliminary qualitative phytochemical analysis. Total phenolic content was estimated by Folin-Ciocalteu method and in vitro antioxidant assays were performed using various radical scavenging activities viz. DPPH, superoxide anion, nitric oxide, hydroxyl and iron chelating activities at different concentrations. The results of the total phenolic content estimation was expressed in terms of equivalence with standard and the antioxidant potentiality of various scavenging and chelating activity were expressed in terms of IC50 values. The results showed that both root and leaves possess significant antioxidant activity even though the former was found to be superior than the latter in terms of parameters assessed. Further the methanol extracts were proved to be an ideal for the materials subjected to investigation.

In-vitro assessment of antioxidant potential of rotula aquatic lour extract

“…Correspondingly, Pallavi et al (2016) observed IC50 values of 91.65μg/ml and 81.98μg/ml in chloroform and methanol extracts of Rotula aquatica roots respectively and IC50 of 99.90 μg/ml and 87.65μg/ml in the leaves extract. Nitric oxide is a diffusible free radical that react with superoxide anion and form potential cytotoxic molecule such as peroxynitrite.…”

“…Similarly, total flavonoid content was found to be 2.60 g and 1.79 g quercetin equivalent/100 g methanolic and aqueous extract respectively of R. aquatic (Pallavi et al, 2016). Hidalgo et al (2010) have reported that flavonoid synergistically interact with free radicals to neutralize them.…”

“…Priya et al (2013) observed a IC50 for methanolic extract of R aquatica to be 65.41 μg/ml and IC50 value of 71.97 μg/ml for aqueous extract. Pallavi et al (2016) reported the methanolic extracts of roots and leaves with IC50 values of 128.27μg/ml and 134.51μg/ml respectively, whereas chloroform extracts of roots and leaves with140.64μg/ml and 198.01μg/ml respectively. Superoxide anion plays a vital role in the production of other reactive oxygen species such as hydrogen peroxide, hydroxyl radicals, or singlet oxygen which induce oxidative damage.…”

“…Priya et al (2013) studied the antioxidant activity of methanolic and aqueous extract of Rotula aquatic stem and reported DPPH IC50 value to be 50.98 μg/ml and 65.4 μg/ml respectively. Pallavi et al (2016) evaluated DPPH scavenging activity of chloroform and methanol extract of R. aquatic root and leaves. The methanolic extracts of roots and leaves were found to possess IC50 values of 128.27μg/ml and 134.51μg/ml respectively, whereas chloroform extracts of roots and leaves showed 50% inhibition at a concentration of 140.64μg/ml and 198.01μg/ml respectively.…”

“…Similarly, Priya et al (2013) has reported the total phenolic content of methanolic extract of R aquatic stem to be 6.8g gallic acid equivalent/100g. Pallavi et al (2016) evaluated chloroform and methanol extract of R aquatic root and leaves for total phenol content and antioxidant activity. The methanolic extract of root showed highest concentration of phenolics (55.2±0.02 μg/mg) followed by chloroform extract (48.4±0.1μg/mg), while the leaf methanolic and chloroform extract recorded (44.9±0.03 μg/mg) and (34.7±0.3 μg/mg) respectively.…”