Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment

Nowak, J.; Kirsch, Nils; Hegemann, Werner; Stan, Hans‐Jürgen

doi:10.1007/s002530050751

Cited by 55 publications

(42 citation statements)

References 17 publications

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“…In situ aerobic degradation of DCBs by bacteria native to contaminated sites has been documented (Kuhn et al, 1985). (Nowak et al, 1996) under anaerobic conditions.…”

Section: List Of Tablesmentioning

confidence: 99%

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Microbially Mediated Reductive Dechlorination of Dichlorobenzene

Quistorff¹

1999

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Office of Management and Budget, Paperwork Reduction Project (0704-0188), Washington, DC 20503. AGENCY USE ONLY (Leave blank)2. REPORT DATE August 1999 REPORT TYPE AND DATES COVEREDFinal May 1997 -August 1999 TITLE AND SUBTITLEMicrobially Mediated Reductive Dechlorination of Dichlorobenzene AUTHOR(S)Quistorff, Anne S. FUNDING NUMBERSC: F08637-97-C-6013 P: 62202F JON: 1900B56A/2103B58A PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)Cornell The microbially mediated reductive dechlorination of 1,2-, 1,3-, and 1,4-dichlorobenzene (DCB) was studied using site material from eight different locations. The soil and groundwater from each site were anaerobically added to multiple serum bottles and mixed. All three DCB isomers were added to each serum bottle to a total nominal concentration of 30 micro-mole per liter for some microcosms and 600 micro-mole per liter for other microcosms. In addition, electron donor in the form of yeast extract (100 mg/L) was added to some microcosms and some microcosms were autoclaved and served as abiotic controls. The microcosms were then incubated, inverted, in the dark at 24 deg C and periodically monitored by headspace injections into a gas Chromatograph system. Reductive dechlorination of the DCB isomers was noted in microcosms from five of the eight locations and ranged from 10% to nearly 100%. Additionally, in all the positive microcosms, dechlorination proceeded independent of whether yeast extract was added. Due to the rapid onset of dechlorination in some of the microcosms, it is likely that DCB dechlorination is occurring on site. Generally, 1,2-DCB was the nost readily degraded DCB isomer. 1,3-DCB was the next most readily degraded isomer, distantly followed by 1,4-DCB. Reductive dechlorination of the DCB isomers was noted in microcosms from five of the eight locations: Louisiana wetland sediment, Kelly AFB, Robins AFB at well BIA4 at both 17-and 25-foot depths and slight dechlorination for the digested sludge. The extent of MCB production from DCB dechlorination was about 10% SUBJECT TERMSfor the digested sludge and ranged from about 50% to 100% for the other microcosms. The most successful microcosms (exhibiting close to 100% transformation in some instances) were the Louisiana wetland sediment and the Robins AFB well BIA4 at 25-foot depth.Additionally, in all the positive microcosms, dechlorination proceeded independent of whether yeast extract was addedprobably because the indigeno...

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“…In situ aerobic degradation of DCBs by bacteria native to contaminated sites has been documented (Kuhn et al, 1985). (Nowak et al, 1996) under anaerobic conditions.…”

Section: List Of Tablesmentioning

confidence: 99%

“…Methanol, ethanol, acetone and acetate all increased the rate of dechlorination but to a lesser degree and are presented in order of electron donor ability (Nowak et al, 1996).…”

Section: C Anaerobic Degradation Of Chlorinated Benzenesmentioning

confidence: 99%

Microbially Mediated Reductive Dechlorination of Dichlorobenzene

Quistorff¹

1999

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“…These data suggested a slow dehalogenation of HCB in the anaerobic sediment. Reductive dechlorination of HCB has been observed in different anaerobic habitats like soil (Rosenbrock et al, 1997;Brahushi et al, 2004), soil slurry (Ramanand et al, 1993), sediment (Bosma et al, 1988;Beurskens et al, 1993;Prytula and Pavlostathis, 1996;Susarla et al, 1996), sewage sludge (Fathepure et al, 1988;Yuan et al, 1999) and a variety of anaerobic cultures which have been enriched from such anaerobic habitats (Holliger et al, 1992;Masunaga et al, 1996;Nowak et al, 1996;Adrian et al, 1998;Pavlostathis and Prytula, 2000;Wu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Most studies on HCB degradation have been conducted under anaerobic conditions (Holliger et al, 1992;Nowak et al, 1996;Adrian et al, 1998Adrian et al, , 2000Pavlostathis and Prytula, 2000;Wu et al, 2002), and only few studies have under aerobic biodegradation processes (Chen et al, 2002;Gan et al, 2001). No strictly aerobic organism capable of degrading HCB has been isolated (Chen et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Aerobic biodegradation of hexachlorobenzene by an acclimated microbial community

Liu

Chen

Shen

2009

IJEP

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Abstract:The aerobic degradation of hexachlorobenzene (HCB) by an acclimated microbial community which isolated from a contaminated site and acclimated in our laboratory was investigated. The enriched microbial community was capable of biodegrading HCB when cultivated in minimal salts medium and supplied HCB as the sole carbon source. The efficiencies of microbial community in the degradation of HCB under different pH and temperatures were examined. The phylogenetic analysis for the nearly complete sequences of 16S rDNA demonstrated that the bacteria assemblage in the microbial community was dominated by Azospirillum and Alcaligenes groups.

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“…Anthropogenic production and uncontrolled release of CBs in addition to their high persistence and bioaccumulation has resulted in broad distribution of these pollutants in the environment [326][327][328]. Partial dechlorination of highly chlorinated benzenes has been shown in a broad range of suboxic/anoxic environments such as soil [329,330], river sediment [331][332][333], sewage sludge [334,335] and drainage ditch [57] with lower chlorinated benzenes as end products. Even though monochlorobenzene (MCB) was considered to be recalcitrant to biotransformation in anoxic environments [8], several studies reported further dechlorination of MCB to benzene in anaerobic enrichments originated from freshwater sediments [333,336,337].…”

Section: Introductionmentioning

confidence: 99%

Ecophysiology and environmental distribution of organohalide-respiring bacteria

Lu¹

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Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment

Cited by 55 publications

References 17 publications

Microbially Mediated Reductive Dechlorination of Dichlorobenzene

Microbially Mediated Reductive Dechlorination of Dichlorobenzene

Aerobic biodegradation of hexachlorobenzene by an acclimated microbial community

Ecophysiology and environmental distribution of organohalide-respiring bacteria

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