Total Saponins from Nigella glandulifera Seeds Ameliorate Adjuvant-Induced Rheumatoid Arthritis in Rats by Inhibition of an Inflammatory Response and Bone Erosion

Zeng, Li; Li, Chenyang; Jiang, Hailun; Yan, Chen; Li, Zhuorong; Xu, Fang; Liu, Rui

doi:10.1155/2021/6613527

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…Enzyme-Linked Immunosorbent Assay (ELISA). The levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), 3-nitrotyrosine (3-NT), 4-hydroxynonenal (4-HNE), and interleukin-1β (IL-1β) in the homogenates of the cortex and in the supernatant of hNCs were measured using corresponding ELISA kits (Proteintech, Rosemont, IL, USA; RayBiotech, Inc., Norcross, GA, USA; Nanjing Jiancheng Bioengineering Institute) [25][26][27][28][29]. Data are presented as pg/mL.…”

Section: Detection Of Apoptosismentioning

confidence: 99%

Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p‐CaMKII/ERK/CREB and ox‐CaMKII‐Dependent MAPK/NF‐κB Pathways

Jiang

Ashraf

Liu

et al. 2021

Oxidative Medicine and Cellular Longevity

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Vascular dementia (VaD) is a common cause of cognitive decline and dementia of vascular origin, but the precise pathological mechanisms are unknown, and so effective clinical treatments have not been established. Tilianin, the principal active compound of total flavonoid extract from Dracocephalum moldavica L., is a candidate therapy for cardio-cerebrovascular diseases in China. However, its potential in the treatment of VaD is unclear. The present study is aimed at investigating the protective effects of tilianin on VaD and exploring the underlying mechanism of the action. A model of VaD was established by permanent 2-vessel occlusion (2VO) in rats. Human neurons (hNCs) differentiated from human-induced pluripotent stem cells were used to establish an oxygen-glucose deprivation (OGD) model. The therapeutic effects and potential mechanisms of tilianin were identified using behavioral tests, histochemistry, and multiple molecular biology techniques such as Western blot analysis and gene silencing. The results demonstrated that tilianin modified spatial cognitive impairment, neurodegeneration, oxidation, and apoptosis in rats with VaD and protected hNCs against OGD by increasing cell viability and decreasing apoptosis rates. A study of the mechanism indicated that tilianin restored p-CaMKII/ERK1/2/CREB signaling in the hippocampus, maintaining hippocampus-independent memory. In addition, tilianin inhibited an ox-CaMKII/p38 MAPK/JNK/NF-κB associated inflammatory response caused by cerebral oxidative stress imbalance in rats with VaD. Furthermore, specific CaMKIIα siRNA action revealed that tilianin-exerted neuroprotection involved increase of neuronal viability, inhibition of apoptosis, and suppression of inflammation, which was dependent on CaMKIIα. In conclusion, the results suggested the neuroprotective effect of tilianin in VaD and the potential mechanism associated with dysfunction in the regulation of p-CaMKII-mediated long-term memory and oxidation and inflammation involved with ox-CaMKII, which may lay the foundation for clinical trials of tilianin for the treatment of VaD in the future.

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Section: Detection Of Apoptosismentioning

confidence: 99%

Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p‐CaMKII/ERK/CREB and ox‐CaMKII‐Dependent MAPK/NF‐κB Pathways

Jiang

Ashraf

Liu

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…FOXP3, TNF-α, IL-17A, IL-6, and IFN-γ are important for the remission and relapse phases of RA [ 18 , 19 ] and, as previously reported, PDCD5 up-regulated FOXP3 and cytokines in rat models of RA [ 5 ]. To estimate the role of these factors in the increased expression of PDCD5 in RA patients, the expression levels of FOXP3 , TNF-α , IL-17A , IL-6 , and IFN-γ were determined.…”

Section: Resultsmentioning

confidence: 70%

PDCD5 as a Potential Biomarker for Improved Prediction of the Incidence and Remission for Patients with Rheumatoid Arthritis

Xiao,

Zhou,

Zhao

et al. 2023

Rheumatol Ther

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Introduction Rheumatoid arthritis (RA) often involves an altered T-cell subpopulation, higher levels of inflammatory cytokines, and auto-antibodies. This study investigated whether PDCD5 could be a biomarker to predict the incidence and remission of RA so as to guide the therapeutic management of clinical RA. Methods One hundred fifty-two patients (41 being in both active status and stable remission status) who were newly diagnosed with RA and 38 healthy controls were enrolled. Basic clinical data were collected before using blood samples remaining in the clinic after routine complete blood count. The ability of PDCD5 and important indicators to predict the remission of RA was estimated based on receiver operating characteristic curve (ROC) analysis. Results PDCD5 expression was found to be significantly increased in RA patients in active status in comparison with healthy controls or those in stable remission status. Compared with anti-CCP, ESR and DAS28 score, PDCD5 was of better predictive value with an AUC of 0.846 (95% CI 0.780–0.912) for RA remission. The incidence risk of RA increased with higher levels of PDCD5 (OR = 1.73, 95% CI = 1.45–1.98, P = 0.005) in multiple logistic regression analysis, with the risk increasing by 2.94-times for high-risk group in comparison with low-risk group (OR = 2.94, 95% CI = 2.35–4.62, P < 0.001). The association between PDCD5 and RA remission showed a similar result. For correlation analysis, significant associations were eventually found between PDCD5 and indicated genes (FOXP3, TNF-α, IL-17A, IFN-γ and IL-6) as well as several important clinical parameters including IgG, RF, CRP, ESR, anti-CCP and DAS28 score. Conclusions This study suggested that increased PDCD5 expression was significantly linked to the incidence and remission of RA. PDCD5 may be used as a novel biomarker for the prediction of RA incidence and remission, especially due to its potential involvement in the development of the condition.

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“…RA has a complex set of causes and is the most common autoimmune rheumatic illness. Damage to cartilage and bone as well as articular deformity are hallmarks of the condition, which is caused by the immune system's persistent infiltration of the synovial membrane (11) . We aimed in our study to evaluate the relation between the disease activity of active rheumatoid cases under conventional medical treatment, and the level of Gal-1 in serum.…”

Section: Discussionmentioning

confidence: 99%

Serum Level of Galactin-1 Association with Disease Activity of Rheumatoid Arthritis Patients under Conventional Treatment

Rahman¹,

Thabet²,

Metwely³

2022

The Egyptian Journal of Hospital Medicine

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Background: The small joints of feet, hands and wrists are particularly vulnerable to impacts of Rheumatoid Arthritis (RA), the most prevalent auto-inflammatory disorder. The pathogenesis of RA is still unknown which leads to the ongoing studies for further understands the pathogenesis and find new treatment targets. Galectin family includes Galectin-1 (Gal-1). It could be found in a large number of tissues with pro-inflammatory as well as anti-inflammatory effects relying on the cellular microenvironment. Objectives: The aim of the current study is to evaluate the relation between the disease activity of active rheumatoid cases under conventional medical treatment, and the level of Gal-1 in serum. Patient and methods: A total of 50 healthy controls and 50 RA patients; at least 18 years old using conventional medical treatment, were included in this case control study. Clinical evaluation, laboratory testing, serum Gal-1 levels, and evaluation of disease activity, was performed to all participants. Results: At a cutoff of >15 (ng/ml), the serum Gal-1 level in the RA group was significantly higher compared to the healthy control group. Serum Gal-1 was not significantly correlated with the activity of RA. Conclusion: Our results demonstrated the usefulness of measuring the serum levels of Galectin-1 in patients with RA with high validity. This proves the pro-inflammatory effect of Galectin-1 in RA patients.

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Total Saponins from Nigella glandulifera Seeds Ameliorate Adjuvant-Induced Rheumatoid Arthritis in Rats by Inhibition of an Inflammatory Response and Bone Erosion

Cited by 6 publications

References 39 publications

Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p‐CaMKII/ERK/CREB and ox‐CaMKII‐Dependent MAPK/NF‐κB Pathways

Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p‐CaMKII/ERK/CREB and ox‐CaMKII‐Dependent MAPK/NF‐κB Pathways

PDCD5 as a Potential Biomarker for Improved Prediction of the Incidence and Remission for Patients with Rheumatoid Arthritis

Serum Level of Galactin-1 Association with Disease Activity of Rheumatoid Arthritis Patients under Conventional Treatment

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