Total starch in animal feeds and silages based on the chromatographic determination of glucose

Murillo, Marcela María Salazar; Granados-Chinchilla, Fabio

doi:10.1016/j.mex.2018.01.009

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…For the starch quantification, 100 mg of powder per sample was analyzed using an a-amylase/amyloglucosidase starch assay kit (K-TSTA, Megazyme, Ireland) according to the manufacturer’s instructions. The starch content of the mature seeds was determined as has been previously described by [ 60 ]. For the protein quantification, 50 mg of powder was used for the protein extraction according to the method described by [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

Wang

et al. 2023

Plants

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The kernel serves as a storage organ for various nutrients and determines the yield and quality of maize. Understanding the mechanisms regulating kernel development is important for maize production. In this study, a small-kernel mutant smk7a of maize was characterized. Cytological observation suggested that the development of the endosperm and embryo was arrested in smk7a in the early development stage. Biochemical tests revealed that the starch, zein protein, and indole-3-acetic acid (IAA) contents were significantly lower in smk7a compared with wild-type (WT). Consistent with the defective development phenotype, transcriptome analysis of the kernels 12 and 20 days after pollination (DAP) revealed that the starch, zein, and auxin biosynthesis-related genes were dramatically downregulated in smk7a. Genetic mapping indicated that the mutant was controlled by a recessive gene located on chromosome 2. Our results suggest that disrupted nutrition accumulation and auxin synthesis cause the defective endosperm and embryo development of smk7a.

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Section: Methodsmentioning

confidence: 99%

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

Wang

et al. 2023

Plants

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“…Starch quantification is carried out by first extracting the sample with water or ethanol followed by filtration and subjection into the HPLC column. The column is typically equipped with a refractive index detector (RID), which detects changes in the refractive index of the eluent as the sample components pass through the column [103,104]. Excessive fillers destroy the authenticity of the spices, and to detect the fillers in the powdered spices, HPLC is an alternate solution.…”

Section: Hplc Quantification Of Starch and Other Fillers In Spicesmentioning

confidence: 99%

Current Trends in HPLC for Quality Control of Spices

Sahu¹,

Purohit²,

Tripathy³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

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India, the land of spices and condiments, is endowed with a plethora of herbs, spices, and unusual plants. Spices have been used as flavoring and coloring agents in Indian society since time immemorial. Spices have also been shown to have antioxidant, antibacterial, anticancer, and anti-inflammatory properties. Assessing spices’ taste, nutritional, and bioactive qualities during postharvest processing is critical for quality control and preventing adulteration. Various illegal colors are frequently used to adulterate spices for fraudulent trading operations. For instance, Sudan dyes are widely substituted with hot chili, red pepper, or tomato products; metanil yellow in turmeric; tartrazine, amaranth, and sunset yellow FCF in ginger and chili powder; and magenta III and rhodamine B in saffron. These adulterants degrade the flavoring, fragrance, cosmetics, medicinal, and preservative value of spices, their authentication is critical in quality control. Apart from these adulterants, various aflatoxins secreted after fungal contamination also cause quality degradation of spices. According to the literature evaluation, HPLC is a rapid and adaptable technique for efficiently identifying these compounds in spices. The proposed chapter summarizes application of HPLC for detection, quantification, and quality assessment of various spices. Some of the recently published work on the said topic from various search engines (Google scholar, Scopus, science direct, etc.) is mentioned in the chapter.

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“…One could also cite a series of organically modified porous Zn imidazolate solids [101]. In the case of functions, flexible MOFs will not only modify the host and guest interactions, but will also drastically affect the flexibility of the MOF during the adsorption or delivery of the biomolecule [102,103] (Figure 10).…”

Section: View Of the Structures Of A Few Mofs Based On Endogenous Lin...mentioning

confidence: 99%

“…Because APCI produces a single charged product, the molecular mass spectrum of the product can be directly observed. In contrast to ESI mode, LC coupled to positive or negative ion mode APCI is more suitable for the analysis of weakly polar or nonpolar compounds due to the sample vaporization [102,103]. For identification of anthocyanins with the same molecular mass, either sample treatment such as acid hydrolysis must be performed to release the anthocyanidin aglycons or MS fragmentation data must be obtained.…”

Section: Electrospray Ionization (Esi) Mass Spectrometry (Ms): Esi-msmentioning

confidence: 99%

High Performance Liquid Chromatography - Recent Advances and Applications

2023

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Increasing interest in topics related to health and quality of life in recent years has led to a growing need in food, environmental and bioanalytical research for high-throughput separation techniques able to cope with the qualitative/quantitative determination of a large number of compounds in very complex matrices. High-performance liquid chromatography (HPLC) is a well-established separation technique widely employed in many fields. The versatility of chromatographic separation modes, coupled with low-resolution and high-resolution mass spectrometry, makes HPLC among the best options to solve emerging analytical problems.This book provides an overview of new advances in high-performance liquid chromatography and its applications in different fields. The first of the book's two sections is devoted to principles and new advances in HPLC. In Chapter 1, "Principles and Applications of Ultra-High-Performance Liquid Chromatography", Ahmed et al. describe the fundamentals of UHPLC techniques (solvent delivery systems, columns, sample injection, and detection), and review the role of UHPLC in different fields such as the analysis of herbal medicines, drugs in human plasma, pharmacokinetics and bioavailability studies, identification of metabolites (metabolomics), detection of impurities, analysis of dosage formulations, food safety and agricultural applications.The higher separation capacity of 2D-LC techniques can be especially useful. A commercial application, the TurboFlow technology developed by Thermo Fisher Scientific, may enable the direct introduction of biological samples into an online automated extraction system without any pre-treatment. In Chapter 2, "Turbulent Flow Chromatography: A Unique Two-Dimensional Liquid Chromatography", Di Gaudio et al. explain the principles of turbulent flow chromatography, the different column chemistries available, the hardware employed, how to operate in multichannel systems, and how to develop a focus mode method to implement this technique. They also describe successful applications of the turbulent flow technique in different fields, including therapeutic drug monitoring and environmental analysis, and applied to different matrices such as urine, plasma, food commodities and water.Monolithic media are excellent substitutes for conventional particle-packed columns. This is because monolithic columns show higher permeability and lower flow resistance than conventional liquid chromatography columns, providing high-throughput performance, resolution and separation in short run times. In Chapter 3, "Monoliths Media: Stationary Phases and Nanoparticles", Hernawy et al. address the general characteristics and properties of monolithic materials, the separation mechanism involved, and the different types of monoliths: organic polymer, silica and metal. Applications of monolithic materials in LC separations and nanoparticle-based monoliths are also described.In Chapter 4, "Perspective Chapter: High-performance Liquid Chromatography Coupled to Mass Spectrometry -The Advance in Che...

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Total starch in animal feeds and silages based on the chromatographic determination of glucose

Abstract: Graphical abstract

Cited by 10 publications

References 13 publications

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

Current Trends in HPLC for Quality Control of Spices

High Performance Liquid Chromatography - Recent Advances and Applications

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