Total tissue lactate dehydrogenase activity in endometrial carcinoma

Šimaga, Šumski; Abramić, Marija; Osmak, Maja; Babić, Damir; Ilić-Forko, Jadranka

doi:10.1111/j.1525-1438.2008.01196.x

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…The method itself and the obtained results can be repeated and checked with high reliability. Such an LDH isoenzyme profile in malignant tumours is in accordance with findings of other authors [22,23]. More recent research [24] has highlighted the great importance of determining the total activity of LDH and isoenzyme profile in diagnostics and treatment of patients with testicular cancer.…”

Section: Discussionsupporting

confidence: 89%

Comparison of glycolytic enzyme and isoenzyme activity in breast cancers and dysplasia

Katić

2012

Med pregl

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The study was aimed at assessing the total enzyme activity and the profile of breast cancer and dysplasia on the human material. In addition, the validity of data was evaluated from the aspect of improving diagnostics. Lactate dehydrogenase activity, as well as the profile of its isoenzymes, pyruvate kinase and hexokinase, were measured. The study included 60 samples of breast cancer, out of which 20 were benign breast tumours and 40 were 1st and 2nd degree dysplasia of the breast. The samples were collected from the patients operated at the Institute for Oncology of Faculty of Medicine in Sremska Kamenica. Lactate dehydrogenase isoenzymes were separated by the vertical polyacrylamide gel disc electrophoresis according to the slightly modified Brewer and Ashworth’s method. The activity of all the tested enzymes was measured under the conditions of linear kinetics in the function of time and enzyme concentration. Lactate dehydrogenase-5 was found in 88% of the analyzed breast cancer samples, whereas it was not detected in breast dysplasia. Pyruvate kinase (4.-isoenzyme) was about 50 times higher and the activity of hexokinase was 3 times higher in breast cancer than in breast dysplasia. Lactate dehydrogenase-5 and pyruvate kinase (4.-isoenzyme) are particularly important and reliable markers of malignity. The results obtained for quantitative and qualitative changes in the enzyme activity can be used to improve diagnostics and early diagnostics of malignant breast neoplasm

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Section: Discussionsupporting

confidence: 89%

Comparison of glycolytic enzyme and isoenzyme activity in breast cancers and dysplasia

Katić

2012

Med pregl

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“…16. Briefly, a lysate was prepared from 10 5 cells using modified RIPA lysis buffer (50 mM Tris, pH 7.8, 150 mM NaCl, 5 mM EDTA, 15 mM MgCl 2 , 1% Nonidet P-40, 0.5% sodium deoxycholate) and added to an assay mixture containing 0.22 mM reduced ␤-nicotinamide adenine dinucleotide (NADH) in 0.2 M KCl buffer containing 40 mM HEPES, pH 7.1, pre-warmed to 37°C.…”

Section: Methodsmentioning

confidence: 99%

Kinetic Modeling of Hyperpolarized 13C Label Exchange between Pyruvate and Lactate in Tumor Cells

Witney¹,

Kettunen

Brindle

2011

Journal of Biological Chemistry

137

203

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Measurements of the kinetics of hyperpolarized 13 C label exchange between [1-13 C]pyruvate and lactate in suspensions of intact and lysed murine lymphoma cells, and in cells in which lactate dehydrogenase expression had been modulated by inhibition of the PI3K pathway, were used to determine quantitatively the role of enzyme activity and membrane transport in controlling isotope flux. Both steps were shown to share in the control of isotope flux in these cells. The kinetics of label exchange were well described by a kinetic model that employed rate constants for the lactate dehydrogenase reaction that had been determined previously from steady state kinetic studies. The enzyme showed pyruvate inhibition in steady state kinetic measurements, which the kinetic model predicted should also be observed in the isotope exchange measurements. However, no such pyruvate inhibition was observed in either intact cells or cell lysates and this could be explained by the much higher enzyme concentrations present in the isotope exchange experiments. The kinetic analysis presented here shows how lactate dehydrogenase activity can be determined from the isotope exchange measurements. The kinetic model should be useful for modeling the exchange reaction in vivo, particularly as this technique progresses to the clinic.Magnetic resonance spectroscopy is a powerful tool for the non-invasive investigation of cellular metabolism, in systems ranging from isolated cells, through perfused organs (1), and small animals to humans (2, 3). A fundamental limitation of magnetic resonance spectroscopy has been a lack sensitivity, which limits temporal resolution to the minute time scale and spatial resolution to 1-10 cm 3 , depending on the magnetic field strength and nucleus detected (4). The recent introduction of dissolution dynamic nuclear polarization (5), which can increase the sensitivity of the magnetic resonance experiment by more than 10,000-fold, has had a significant impact on the field (reviewed in Refs. 6 and 7), dramatically increasing the temporal resolution to the second time scale and the spatial resolution to the millimeter scale. With this technique the 13 C nucleus, in a 13 C-labeled cell metabolite, is hyperpolarized and then injected into the biological system, where this may be an intravenous injection into a small animal or human. The gain in sensitivity, due to hyperpolarization of the 13 C nucleus, means that there is now sufficient signal to image the distribution of the labeled metabolite in the body and, more importantly, its metabolism and conversion into other cellular metabolites. The main limitation is the relatively short lifetime of the polarization, which in the 13 C-labeled metabolites, which have been used to date, is between 10 and 30 s. This means that spectroscopic imaging must be accomplished within 2-3 min and the labeled molecule must be taken up by cells and metabolized very rapidly for a significant labeling of other cell metabolites within the lifetime of the polarization. Although other nuclei ca...

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“…The lactate dehydrogenase activity in serum was measured with little modification of the protocol of Simaga et al., 2008 ( 28 ). The activity was measured spectrophotometrically at room temperature for 3 min by recording decrease in absorbance of NADH at 340 nm in UV 1900 Shimadzu UV visible double beam spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Olive leaves extract alleviates inflammation and modifies the intrinsic apoptotic signal in the leukemic bone marrow

et al. 2023

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IntroductionCurrent anti-leukemic chemotherapies with multiple targets suffer from side effects. Synthetic drugs with huge off-target effects are detrimental to leukemic patients. Therefore, natural plant-based products are being increasingly tested for new anti-leukemic therapy with fewer or no side effects. Herein, we report the effect of ethanolic olive leaves extract (EOLE) on the K562 cell line and on the bone marrow (BM) of N-ethyl-N-nitrosourea (ENU)-induced leukemic mice.MethodsUsing standard methodologies, we assessed viability, chromatin condensation, and induction of apoptosis in EOLE-treated K562 cells in-vitro. The anti-leukemic activity of EOLE was assayed by measuring ROS, levels of various cytokines, expression of iNOS and COX-2 gene, and changes in the level of important apoptosis regulatory and cell signaling proteins in-vivo. ResultK562 cells underwent apoptotic induction after exposure to EOLE. In the BM of leukemic mice, EOLE therapy decreased the number of blast cells, ROS generation, and expression of NF-κB and ERK1/2. IL-6, IL-1β, TNF-α, iNOS, and COX-2 were among the inflammatory molecules that were down-regulated by EOLE therapy. Additionally, it decreased the expression of anti-apoptotic proteins BCL2A1, BCL-xL, and MCL-1 in the BM of leukemic mice.DiscussionChronic inflammation and anomalous apoptotic mechanism both critically contribute to the malignant transformation of cells. Inflammation in the tumor microenvironment promotes the growth, survival, and migration of cancer cells, accelerating the disease. The current investigation showed that EOLE treatment reduces inflammation and alters the expression of apoptosis regulatory protein in the BM of leukemic mice, which may halt the progression of the disease.

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Total tissue lactate dehydrogenase activity in endometrial carcinoma

Cited by 10 publications

References 26 publications

Comparison of glycolytic enzyme and isoenzyme activity in breast cancers and dysplasia

Comparison of glycolytic enzyme and isoenzyme activity in breast cancers and dysplasia

Kinetic Modeling of Hyperpolarized 13C Label Exchange between Pyruvate and Lactate in Tumor Cells

Olive leaves extract alleviates inflammation and modifies the intrinsic apoptotic signal in the leukemic bone marrow

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