Total urine antioxidant capacity

Kirschbaum, Barry B.

doi:10.1016/s0009-8981(01)00381-3

Cited by 46 publications

(41 citation statements)

References 13 publications

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“…3 For this reason, the assessment of total antioxidant capacity (TAC) of biological fluids will be a useful biomedical tool that can assist the evaluation of physiological, environmental and nutritional factors that influence the human redox status. 4 In this regard, several human biological fluids have been used as samples for the in vitro evaluation of TAC, including plasma, [5][6][7][8][9][10][11][12] serum, [13][14][15][16] urine, 8,12,17 saliva, 15 cerebrospinal fluid, 18 tears, 19 breast milk 20 and also semen. 21 Various batch methodologies based on electron spin resonance (ESR), 10 electrochemical, 6,11 chemiluminescence (CL), 8,15,16 fluorometric, 7 and UV-Vis spectrophotometric 5,13,14 detection have been proposed.…”

Section: Introductionmentioning

confidence: 99%

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

et al. 2011

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High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a microformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h -1 (microplate method) or of 15 h -1 (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.

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Section: Introductionmentioning

confidence: 99%

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

et al. 2011

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“…Also the negative correlation between semi quantitative colony count in urine and plasma Thiols was very significant (r value= -0.70, p<0.001,Table1) whereas the same was found to be weakly significant in the control group (r value= 0.40, p<0.001, Table 2) Licensed Under Creative Commons Attribution CC BY antioxidant enzymes measured quantitatively were depleted in response to oxidative stress. Kirschbaum reported that total antioxidant enzyme activity was lower in patients with acute renal disease compared to those of control urine specimen [4]. In our study the results show that UTI may cause oxidative stress by consuming urinary antioxidant enzymes and it is possible to say that urinary antioxidant enzymes are not enough to prevent the oxidative stress in UTI.…”

Section: Resultsmentioning

confidence: 42%

Oxidative Stress in Urinary Tract Infection

2017

IJSR

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Aerobic respiration and oxidation of bio molecules used as substrates, lead to production of reactive oxygen species (ROS) to combat which we have antioxidant defence mechanisms in our body. Lipid peroxidation is one of the most important expression of oxidative stress induced by ROS. Malonaldehyde (MDA), a product of lipid peroxidation along with antioxidant protein thiols are expressed in plasma. Urinary tract infections (UTIs) may appear in all age groups due to asymptomatic bacteria. The present study aims to determine the relationship of antioxidant levels and oxidative stress in UTI. 60 subjects between the age group 35-60 years who were diagnosed with Urinary Tract Infection and attended the OPD along with 40 normal healthy subjects (as controls) were included in this study. Midstream urine was sent to microbiology laboratory for diagnosis of UTI. Macroscopic appearance of urine was also studied. MDA were assessed by thiobarbituric acid as a substrate and thiol were estimated by Ellmann's method. Data were analyzed using Statistical Package for the Social Sciences (SPSS 16). In this study we found that plasma MDA level was significantly higher and plasma thiols significantly lower in positive urine cultures compared to negative urine cultures and may indicate the existence of oxidative stress. Since MDA and protein thiols test results can be obtained before the results of cultures taken in urinary tract infection, it may be used as a diagnostic tool and may contribute to the initiation of treatment without waiting for the culture results.

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“…Plasma protein antioxidants such as albumin are not normally lost in the urine to any appreciable extent, but certain diseases feature large urinary losses in circulating levels [12] . A variety of oxidation products are found in urine and are thought to mirror local and systemic oxidative stress [13] . Various diseases accompany many inflammatory conditions and influence the endogenous antioxidant enzyme activities.…”

Section: Discussionmentioning

confidence: 99%

Decreased Oxidative Stress Index of Urine in Patients with Urinary Tract Infection

Çiftçi¹,

Verit²,

Yeni³

et al. 2008

Urol Int

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Aim: To investigate the oxidative and antioxidative status of urine and serum of patients with urinary tract infection (UTI) and to compare them with those of controls. Materials and Methods: The antioxidative status of urine and serum were evaluated by measuring total antioxidant capacity (TAC) and plasma vitamin C concentration. The oxidative status of the samples was assessed by measuring the total peroxide and the oxidative stress index (OSI) levels. The means of the parameters were compared and the relationships among them were determined. Results: Total peroxide and OSI levels were found to be lower and TAC was found to be higher in the urine of patients with UTI. On the contrary, while the plasma vitamin C concentration and TAC of serum was lower, the total peroxide and OSI levels were higher in patients than controls. There was a negative correlation between serum TAC and urine TAC values. There was also a positive correlation between plasma vitamin C concentration and serum TAC levels. Conclusion: The urine of the patients with UTI have higher TAC and lower total peroxide and OSI levels. However, lower TAC and plasma vitamin C concentration and higher total peroxide and OSI levels were observed in UTI. This condition may be a factor which facilitates the development of the infection or is secondary to UTI.

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Total urine antioxidant capacity

Cited by 46 publications

References 13 publications

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

Oxidative Stress in Urinary Tract Infection

Decreased Oxidative Stress Index of Urine in Patients with Urinary Tract Infection

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