Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. ch 1039)

Abstract

Embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (Oryza sativa L. cv. CH 1039). Compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. Murashige and Skoog's (MS) medium supplemented with 2.5mg/1 2,4-D was used as callus induction medium. Plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to MS basal medium supplemented with 2.0mg/1 BAP and 0.5mg/1 NAA in combination. Average number of regenerated plants from one coleoptile ranged from9.1 to 14.0.Four day old coleoptiles showed the highest frequency of plant regeneration.

High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

“…The stimulatory effect of 2,4-D at 11.3 M on callus induction from immature basal portion of rice leaf segment (Abdullah et al, 1986) and 9.0 M in inducing regenerable calli from leaf blade segments of rice (Yan and Zhao, 1982) was successfully demonstrated. Similarly 2,4-D at concentrations between 9.0 and 11.3 M was found to be satisfactory in calli induction from leaf segments of African perennial rice, Oryza longistaminata (Boissot et al, 1990) and coleoptile segments of indica rice (Oinam and Kothari, 1995). 2,4-D at concentrations between 4.52 and 11.3 M facilitated embryogenic callus induction in somatic tissues of indica and japonica rice varieties (Kavi Kishore and Reddy, 1986).…”

“…Despite a large number of reports on rice tissue culture, there is significant genotype-dependence, and in vitro regeneration of indica rice is still a challenging task (Kumria et al, 2000). There are several successful reports on regeneration from explants such as leaf blade (Yan and Zhao, 1982), root tips (Sticklen, 1991), leaf sheath/leaf sections (Wernicke and Milkovits, 1984), immature panicles (Shu and Wei, 1980), mature seed embryos (Ramesh and Gupta, 2006), immature embryo (Koetje et al, 1989), coleoptile (Oinam and Kothari, 1995), anthers (Genovesi and Magill, 1982), stem nodes (Furuhashi and Yatazuva, 1964), seedling derived mesocotyl segments (Mascarenhas et al, 1975), leaf segments (Henke et al, 1978) and leaf bases (Ramesh et al, 2009). …”

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

“…For successful carrying out genetic transformation in rice, establishment of efficient plant regeneration in vitro is a pre-requisite [10][11][12]. Till now, different protocols have been developed to initiate callus from explants, such as mature embryos [13][14][15][16][17][18], immature embryos [19,[20][21][22], mature seeds [22][23][24][25], root segments [15,26], coleoptile [27,28] and leaf bases [9]. Genetic engineering is strongly dependent on genotype and availability of an efficient in vitro plant regeneration method.…”

Transient expression of - glucuronidase gene in indica and japonica rice (Oryza sativa L.) callus cultures after different stages of co-bombardment

“…The initiation of callus tissue was observed after 10-14 days from the scutellar region (Figure 2A) of the mature seeds of indica cv Rasi with a frequency of 85.5%. 2,4-D at 11.3 has also been reported to induce optimum callusing in other cultivars of indica and japonica lines (Abdullah et al, 1986;Oinam and Kothari, 1995;Jain et al, 1996;Cao et al ., 1992;Saharan et al ., 2004 exhibited elongation of shoots followed by proliferation of white to yellow callus development on NB medium ( Figure 2C) as observed earlier (Visarada and Sarma, 2002). Portions of friable embryogenic sectors subcultured on fresh medium increased their fresh weight ( Figure 2B) In this study, friable, nodular, white to pale yellow embryogenic calli separated from nonembryogenic portions has been used as starting material ( Figure 2D) to optimize parameters for gene delivery using PDS-1000/He driven particle delivery system.…”