Toward a complete Drosophiladeficiency kit

Roote, John; Russell, Steven

doi:10.1186/gb-2012-13-3-149

Cited by 18 publications

(15 citation statements)

References 9 publications

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“…To elucidate the mechanism by which Da affects cell survival and growth, we performed a dominant genetic modifier screen using a series of chromosomal deletions (Parks et al, 2004; Ryder et al, 2004; Roote and Russell, 2012; Cook et al, 2012) to identify loci that were dose-sensitive for growth effects of high Da (Table S1). The size of the eyg>da eye was modified by reduced gene dose of multiple cell death and cell cycle regulators, including head involution defective , grim , reaper , sickle , string , wee1 , Rb , E2f1 , Cyclin A , and Cyclin E , and was rescued by co-expression of the anti-apoptotic proteins baculovirus p35, Diap1, or dominant-negative Dronc (Table S2).…”

Section: Resultsmentioning

confidence: 99%

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Wang

Baker

2015

Developmental Cell

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The E-proteins and Id-proteins are, respectively, the positive and negative heterodimer partners for the basic-helix-loop-helix protein family, and as such contribute to a remarkably large number of cell fate decisions. E-proteins and Id-proteins also function to inhibit or promote cell proliferation and cancer. Using a genetic modifier screen in Drosophila, we show that the Id-protein Extramacrochaetae enables growth by suppressing activation of the Salvador-Warts-Hippo pathway of tumor suppressors, activation that requires transcriptional activation of the expanded gene by the E-protein Daughterless. Daughterless protein binds to an intronic enhancer in the expanded gene, both activating the SWH pathway independently of the transmembrane protein Crumbs, and bypassing the negative feedback regulation that targets the same expanded enhancer. Thus the Salvador-Warts-Hippo pathway has a cell-autonomous function to prevent inappropriate differentiation due to transcription factor imbalance, and monitors the intrinsic developmental status of progenitor cells, distinct from any responses to cell-cell interactions.

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Section: Resultsmentioning

confidence: 99%

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Wang

Baker

2015

Developmental Cell

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“…One of the distinct advantages of using D . melanogaster is the ability to implicate individual genes in a phenotype by using previously generated genotypes from stock centres such as Vienna Drosophila RNAi Centre [ 54 ], the Bloomington Deficiency Kit [ 55 ] and the MiMic library [ 56 ]. Each stock in these collections contains a defined genetic change in a common background, much like the genotypes discussed in the current study.…”

Section: Discussionmentioning

confidence: 99%

The Wiggle Index: An Open Source Bioassay to Assess Sub-Lethal Insecticide Response in Drosophila melanogaster

et al. 2015

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Toxicological assays measuring mortality are routinely used to describe insecticide response, but sub-lethal exposures to insecticides can select for resistance and yield additional biological information describing the ways in which an insecticide impacts the insect. Here we present the Wiggle Index (WI), a high-throughput method to quantify insecticide response by measuring the reduction in motility during sub-lethal exposures in larvae of the vinegar fly Drosophila melanogaster. A susceptible wild type strain was exposed to the insecticides chlorantraniliprole, imidacloprid, spinosad, and ivermectin. Each insecticide reduced larval motility, but response times and profiles differed among insecticides. Two sets of target site mutants previously identified in mortality studies on the basis of imidacloprid or spinosad resistance phenotypes were tested. In each case the resistant mutant responded significantly less than the control. The WI was also able to detect a spinosad response in the absence of the primary spinosad target site. This response was not detected in mortality assays suggesting that spinosad, like many other insecticides, may have secondary targets affecting behaviour. The ability of the WI to detect changes in insecticide metabolism was confirmed by overexpressing the imidacloprid metabolizing Cyp6g1 gene in digestive tissues or the central nervous system. The data presented here validate the WI as an inexpensive, generic, sub-lethal assay that can complement information gained from mortality assays, extending our understanding of the genetic basis of insecticide response in D. melanogaster.

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“…All mutants were confirmed by genomic polymerase chain reaction and/or sequencing. The initial screen for germ line phenotypes was performed by crossing miRNA mutants to genomic deficiencies (Roote and Russell 2012) uncovering the miRNA loci.…”

Section: Methodsmentioning

confidence: 99%

Maternal Loss of miRNAs Leads to Increased Variance in Primordial Germ Cell Numbers in Drosophila melanogaster

Kugler

Lin

Weng

et al. 2013

G3: Genes, Genomes, Genetics

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MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.

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Toward a complete Drosophiladeficiency kit

Cited by 18 publications

References 9 publications

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

The Wiggle Index: An Open Source Bioassay to Assess Sub-Lethal Insecticide Response in Drosophila melanogaster

Maternal Loss of miRNAs Leads to Increased Variance in Primordial Germ Cell Numbers in Drosophila melanogaster

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