Toward a description of the sialome of the adult female mosquito Aedes aegypti

Valenzuela, Jesús G.; Pham, Van My; Garfield, Mark; Francischetti, Ivo M.B.; Ribeiro, José M. C.

doi:10.1016/s0965-1748(02)00047-4

Cited by 175 publications

(201 citation statements)

References 61 publications

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“…These include two clusters coding for proteins with high similarity to the antigen 5 family, which is a widespread extracellular family of proteins found in animals and plants (Schreiber et al, 1997), most of unknown function. Members of this protein family are found in salivary glands of sand flies, mosquitoes, and tsetse (Charlab et al, 1999;Li et al, 2001;Valenzuela et al, 2001Valenzuela et al, , 2002cFrancischetti et al, 2002b) and are here described for the first time in the salivary glands of a Hemiptera. One cluster contains a sequence coding for a low complexity basic protein with a clear signal peptide, indicative of secretion.…”

Section: Description Of Clusters Associated With Transcripts Having Pmentioning

confidence: 76%

“…Bioinformatics procedures were as by Francischetti et al (2002b) and Valenzuela et al (2002c) except that the clustering of the cDNA sequences was accomplished using the CAP program (Huang, 1992) after initial clustering of the database following a blastn (Altschul et al, 1997) of the database against itself and reading the output to join those sequences that had at least 95 identical residues in a window of 100 residues. Accession numbers for the National Center for Biology Information (NCBI) databases are given as gi|XXXX where XXXX is the accession number.…”

Section: Sequencingmentioning

confidence: 99%

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Exploring the sialome of the blood-sucking bug Rhodnius prolixus

Ribeiro

Andersen

Silva-Neto

et al. 2004

Insect Biochemistry and Molecular Biology

140

160

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Section: Description Of Clusters Associated With Transcripts Having Pmentioning

confidence: 76%

Section: Sequencingmentioning

confidence: 99%

Exploring the sialome of the blood-sucking bug Rhodnius prolixus

Ribeiro

Andersen

Silva-Neto

et al. 2004

Insect Biochemistry and Molecular Biology

140

160

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“…For example, in Ae. aegypti, calreticulins, fibrinogen/fibronectins, serpins, phosphatidylethanolamine-binding proteins, odorant-binding proteins, alkaline phosphatases, lectins, and hydrolases are expressed in both the male AG/ED and the female salivary glands (Valenzuela et al, 2002;. Genes encoding proteins in these classes are also expressed in the salivary glands and digestive tracts of other blood-feeding arthropods (Jaworski et al, 1995;Ribeiro et al, 2004;Arca et al, 2005;Andersen et al, 2007).…”

Section: 5g Novel Proteins-eight Of the Putativementioning

confidence: 99%

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

Sirot

Poulson

McKenna

et al. 2008

Insect Biochemistry and Molecular Biology

155

166

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Male reproductive gland proteins (mRGPs) impact the physiology and/or behavior of mated females in a broad range of organisms. We sought to identify mRGPs of the yellow fever mosquito, Aedes aegypti, the primary vector of dengue and yellow fever viruses. Earlier studies with Ae. aegypti demonstrated that "matrone" (a partially purified male reproductive accessory gland substance) or male accessory gland fluid injected into virgin female Ae. aegypti affect female sexual refractoriness, blood feeding and digestion, flight, ovarian development, and oviposition. Using bioinformatic comparisons to Drosophila melanogaster accessory gland proteins and mass spectrometry of proteins from Ae. aegypti male accessory glands and ejaculatory ducts (AG/ED) and female reproductive tracts, we identified 63 new putative Ae. aegypti mRGPs. Twenty-one of these proteins were found in the reproductive tract of mated females, but not of virgin females, suggesting that they are transferred from males to females during mating. Most of the putative mRGPs fall into the same protein classes as mRGPs in other organisms, although some appear to be evolving rapidly and lack identifiable homologs in Culex pipiens, Anopheles gambiae, and D. melanogaster. Our results identify candidate male-derived molecules that may have an important influence on behavior, survival and reproduction of female mosquitoes.

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“…Mosquitoes, like all haematophagous arthropods, have evolved mechanisms to effectively neutralize host haemostatic responses, based upon the release of saliva into the feeding site. Saliva is a pharmacologic cocktail of secreted molecules, principally proteins, that can affect vascular constriction, blood coagulation, platelet aggregation, inflammation, immunity, and angiogenesis (Arca et al 1999, Arca et al 2002, Arca et al 2005, Billingsley et al 2006, Calvo et al 2004, Calvo et al 2006a, Calvo et al 2006b, Calvo and Ribeiro 2006, Champagne 2004, Champagne et al 1995, Cross et al 1994, James et al 1991, Kerlin and Hughes 1992, Mellink and Vos 1977, Ribeiro 1987, 1989b, 1992, Ribeiro et al 2000, Ribeiro and Francischetti 2001, 2003, Ribeiro and Nussenzveig 1993, Ribeiro et al 1994, Stark and James 1998, Suwan et al 2002, Valenzuela et al 2002, Wanasen et al 2004, Wasserman et al 2004, Zeidner et al 1999. The saliva of all species of haematophagous arthropod analyzed to date contains at least one anticlotting, one anti-platelet, and one vasodilatory substance.…”

mentioning

confidence: 99%

“…The saliva of all species of haematophagous arthropod analyzed to date contains at least one anticlotting, one anti-platelet, and one vasodilatory substance. Despite recent advances in our knowledge of these molecules and their role in blood-feeding, functions can be ascribed to less than half of the molecules present in mosquito saliva , Valenzuela et al 2002.…”

mentioning

confidence: 99%

The enhancement of arbovirus transmission and disease by mosquito saliva is associated with modulation of the host immune response

Schneider

Higgs

2008

Transactions of the Royal Society of Tropical Medicine and Hygi

227

198

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SUMMARYArthropod-borne viruses have emerged as a major human health concern. Viruses transmitted by mosquitoes are the cause of the most serious and widespread arbovirus diseases worldwide and are ubiquitous in both feral and urban settings. Arboviruses, including dengue and West Nile virus are injected into vertebrates within mosquito saliva during mosquito feeding. Mosquito saliva contains anti-haemostatic, anti-inflammatory and immunomodulatory molecules that facilitate the acquisition of a blood-meal. Collectively, studies investigating the effects of mosquito saliva on the vertebrate immune response suggest that at high concentrations salivary proteins are immmunosuppressive, whereas lower concentrations modulate the immune response; specifically, T H 1 and antiviral cytokines are down-regulated, while T H 2 cytokines are unaffected or amplified. As a consequence, mosquito saliva can impair the anti-viral immune response thus affecting viral infectiousness and host survival. Mounting evidence suggests that this is a mechanism whereby arbovirus pathogenicity is enhanced. In a range of disease models, including various hosts, mosquito species, and arthropodborne viruses, mosquito saliva and/or feeding is associated with a potentiation of virus infection. Compared to arbovirus infection initiated in absence of the mosquito or its saliva, infection including mosquito saliva leads to an increase in virus transmission, host susceptibility, viraemia, disease progression, and mortality.Keywords arthropod-borne virus; mosquito saliva; immunomodulation; disease enhancement Most arthropod-borne (arbo)viruses of public health significance are mosquito-borne, and thus transmitted to vertebrates via the feeding of an infected mosquito. For a mosquito to

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Toward a description of the sialome of the adult female mosquito Aedes aegypti

Cited by 175 publications

References 61 publications

Exploring the sialome of the blood-sucking bug Rhodnius prolixus

Exploring the sialome of the blood-sucking bug Rhodnius prolixus

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

The enhancement of arbovirus transmission and disease by mosquito saliva is associated with modulation of the host immune response

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