Toward a Mouse Neuroethology in the Laboratory Environment

Oliva, Anthony M.; Salcedo, Ernesto E.; Hellier, Jennifer L.; Ly, Xuan; Koka, Kanthaiah; Tollin, Daniel J.; Restrepo, Diego

doi:10.1371/journal.pone.0011359

Cited by 30 publications

(24 citation statements)

References 38 publications

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“…The present results indicate that during plastic responses to chronic odorant exposure, supernumerary glomeruli are added and consolidated as the olfactory bulb matures; no glomerular elimination was seen under any experimental condition (Figure 3 and S3). This result is compatible with the idea that at least under chronic odorant exposure before, during or after glomerular formation, the olfactory bulb uses a constructivist strategy, both at the circuit and glomerular levels, to respond to increased sensory demands; however, this process did not involve an increase of total glomerular volume (Figure 2 and S4); also see [14], [39]. In summary, the present results document a new strategy for the developing olfactory bulb to engange plastic responses.…”

Section: Discussionsupporting

confidence: 87%

Supernumerary Formation of Olfactory Glomeruli Induced by Chronic Odorant Exposure: A Constructivist Expression of Neural Plasticity

et al. 2012

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It is accepted that sensory experience instructs the remodelling of neuronal circuits during postnatal development, after their specification has occurred. The story is less clear with regard to the role of experience during the initial formation of neuronal circuits, whether prenatal or postnatal, since this process is now supposed to be primarily influenced by genetic determinants and spontaneous neuronal firing. Here we evaluated this last issue by examining the effect that postnatal chronic exposure to cognate odorants has on the formation of I7 and M72 glomeruli, iterated olfactory circuits that are formed before and after birth, respectively. We took advantage of double knock-in mice whose I7 and M72 primary afferents express green fluorescent protein and β-galactosidase, correspondingly. Our results revealed that postnatal odorant chronic exposure led to the formation of permanent supernumerary I7 and M72 glomeruli in a dose and time dependent manner. Glomeruli in exposed mice were formed within the same regions of olfactory bulb and occupy small space volumes compared to the corresponding single circuits in non-exposed mice. We suggest that local reorganization of the primary afferents could participate in the process of formation of supernumerary glomeruli. Overall, our results support that sensory experience indeed instructs the permanent formation of specific glomeruli in the mouse olfactory bulb by means of constructivist processes.

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Section: Discussionsupporting

confidence: 87%

Supernumerary Formation of Olfactory Glomeruli Induced by Chronic Odorant Exposure: A Constructivist Expression of Neural Plasticity

et al. 2012

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“…As suggested above, the differences between these and our studies could be due to differences in the strain, age and/or BrdU injection protocol. In addition, a recent report indicated that behavior can be greatly influenced by environmental conditions, making comparison across behavioral studies more difficult (Oliva et al, 2010). Nevertheless, further studies are necessary to determine whether activation of V2R sensory neurons in the VNO by social odors and concomitant activation of the pAOB promotes neurogenesis.…”

Section: Discussionmentioning

confidence: 99%

Regulation of adult neurogenesis by behavior and age in the accessory olfactory bulb

Nunez-Parra

Pugh

Araneda

2011

Molecular and Cellular Neuroscience

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The vomeronasal system (VNS) participates in the detection and processing of pheromonal information related to social and sexual behaviors. Within the VNS, two different populations of sensory neurons, with a distinct pattern of distribution, line the epithelium of the vomeronasal organ (VNO) and give rise to segregated sensory projections to the accessory olfactory bulb (AOB). Apical sensory neurons in the VNO project to the anterior AOB (aAOB), while basal neurons project to the posterior AOB (pAOB). In the AOB, the largest population of neurons are inhibitory, the granule and periglomerular cells (GCs and PGs) and remarkably, these neurons are continuously born and functionally integrated in the adult brain, underscoring their role on olfactory function. Here we show that behaviors mediated by the VNS differentially regulate adult neurogenesis across the anterior-posterior axis of the AOB. We used immunohistochemical labeling of newly born cells under different behavioral conditions in mice. Using a resident-intruder aggression paradigm, we found that subordinate mice exhibited increased neurogenesis in the aAOB. In addition, in sexually naive adult females exposed to soiled bedding odorized by adult males, the number of newly born cells was significantly increased in the pAOB; however, neurogenesis was not affected in females exposed to female odors. In addition, we found that at two months of age adult neurogenesis was sexually dimorphic, with male mice exhibiting higher levels of newly born cells than females. Interestingly, adult neurogenesis was greatly reduced with age and this decrease correlated with a decrease in progenitor cells proliferation but not with an increase in cell death in the AOB. These results indicate that the physiological regulation of adult neurogenesis in the AOB by behaviors is both sex and age dependent and suggests an important role of newly born neurons in sex dependent behaviors mediated by the VNS.

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“…Mice were bred and housed in static micro-isolation cages. A previous study in our laboratory examined two types of caging/housing environments to determine the effects of environment on olfactory function (Oliva et al, 2010). High ventilated caging mechanically exchanges fresh air at a rate of one volume per minute.…”

Section: Methodsmentioning

confidence: 99%

“…Low ventilation or static micro-isolation cages passively exchange air through a filter cover. Oliva et al (2010) noted differences in mouse behavior (e.g., an increase in intermale aggression when housed in “standard”, high ventilated caging) as well as neuroanatomical changes that occurred in the OB as a result of the differences in housing conditions. Therefore, this study housed all mice in static micro-isolation cages.…”

Section: Methodsmentioning

confidence: 99%

Olfactory discrimination varies in mice with different levels of α7-nicotinic acetylcholine receptor expression

et al. 2010

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Previous studies have shown that schizophrenics have decreased expression of α7-nicotinic acetylcholine (α7) receptors in the hippocampus and other brain regions, paranoid delusions, disorganized speech, deficits in auditory gating (i.e., inability to inhibit neuronal responses to repetitive auditory stimuli), and difficulties in odor discrimination and detection. Here we use mice with decreased α7 expression that also show a deficit in auditory gating to determine if these mice have similar deficits in olfaction.In the adult mouse olfactory bulb (OB), α7 expression localizes in the glomerular layer; however, the functional role of α7 is unknown. We show that inbred mouse strains (i.e., C3H and C57) with varying α7 expression (e.g., α7 wild-type [α7+/+], α7 heterozygous knock-out [α7+/−] and α7 homozygous knockout mice [α7−/−]) significantly differ in odor discrimination and detection of chemically related odorant pairs.Using [ 125 I] α-bungarotoxin (α-BGT) autoradiography, α7 expression was measured in the OB. As previously demonstrated, α-BGT binding was localized to the glomerular layer. Significantly more expression of α7 was observed in C57 α7+/+ mice compared to C3H α7+/+ mice. Furthermore, C57 α7+/+ mice were able to detect a significantly lower concentration of an odor in a mixture compared to C3H α7+/+ mice. Both C57 and C3H α7+/+ mice discriminated between chemically related odorants sooner than α7+/− or α7−/− mice. These data suggest that α7-nicotinic-receptors contribute strongly to olfactory discrimination and detection in mice and may be one of the mechanisms producing olfactory dysfunction in schizophrenics.

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Toward a Mouse Neuroethology in the Laboratory Environment

Cited by 30 publications

References 38 publications

Supernumerary Formation of Olfactory Glomeruli Induced by Chronic Odorant Exposure: A Constructivist Expression of Neural Plasticity

Supernumerary Formation of Olfactory Glomeruli Induced by Chronic Odorant Exposure: A Constructivist Expression of Neural Plasticity

Regulation of adult neurogenesis by behavior and age in the accessory olfactory bulb

Olfactory discrimination varies in mice with different levels of α7-nicotinic acetylcholine receptor expression

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