Toward a multigene phylogeny of the Cymatosiraceae (Bacillariophyta, Mediophyceae) II: morphological and molecular insights into the taxonomy of the forgotten species Campylosira africana and of Extubocellulus, with a description of two new taxa

Abstract

New molecular and morphological insights are presented on Campylosira africana and two new species, Extubocellulus cupola sp. nov. and Plagiogrammopsis castigatus sp. nov. Species descriptions were based on LM/SEM micrographs and a data set with concatenated sequences of SSU, rbcL and psbC loci constructed from 56 cymatosiroid strains isolated from global geographic locations. Extubocellulus cupola is distinguished by a mesh‐like marginal ridge and dome‐shaped areolation, and P. castigatus, by a prominent marginal extension at the valve center. Campylosira africana, a species from South Africa described by Professor Malcolm Giffen, has never been documented through LM or SEM, which led some authors to place it in synonymy under Campylosira cymbelliformis. We confirm that C. africana is a distinct species and provide microphotographic documentation and DNA sequences. We present morphological evidence for the well‐known cymatosiroid species Extubocellulus spinifer possessing complete pili, and we emend the generic description of Extubocellulus.

Indonesian coral reef habitats reveal exceptionally high species richness and biodiversity of diatom assemblages

“…Others studies showed that the presence of diatom taxa with broad tropical ranges in coral reefs of the Western Indian Ocean coasts and in the SE Pacific (Li et al 2015(Li et al , 2018). Yet Lobban et al (2012), Ashworth et al (2012), Dąbek et al (2017Dąbek et al ( , 2019, Li et al (2016Li et al ( ,2020; but see also Kryk et al, 2020) have clearly shown numerous taxa limited in their distribution to particular sites. Our analysis of the published research given above involving the use of molecular methods and identification of processed samples allowed us to distinguish three major groups of diatom taxa in terms of their biogeography: cosmopolitan species, including some adapted and also distributed in cold water areas, but rather in the Arctic and Greenland than the Southern Ocean.…”

“…Gottschalk et al, 2007;Hein et al, 2008;Lobban et al, 2012;Desrosiers, 2014;Al Handal et al, 2016;Kryk et al, 2020). Many species and genera have been described based on monoclonal strains and molecular markers have been acquired (Sato, 2008;Ashworth et al, 2012;MacGillivary & Kaczmarska 2012Lobban and Ashworth, 2014;Li et al 2016Li et al , 2018Dąbek et al 2017Dąbek et al , 2019Prasetiya et al, 2019;Witkowski et al, 2020).…”

Lipid Constituents of Diatoms (Halamphora) as Components for Production of Lipid Nanoparticles

“…Strain SZCZM1454A, morphologically identified as Halamphora cf. salinicola, has been isolated from the water sample originating from Köyce giz Lake, Dalyan Channel in Turkey (36 • 50 34.4" N, 28 • 37 54.6" E), using micropipette technique under the inverted LM described in [25,26]. The diatom was grown in Petri dish enriched with artificial f/2 culture medium [27] with salinity adjusted to 35‰ and kept in a plant growth chamber (FITO1400i, Biogenet, Poland) with a 12:12-h light:dark photoperiod at 20 • C and illuminated with ca.…”

The blue diatom Haslea ostrearia from the Indian Ocean coast of South Africa, with comparative analysis of Haslea organellar genomes

“…10 days. A subsample of the clonal culture biomass was then cleaned and boiled with 37% hydrogen peroxide and microscopic slides were prepared as described in Dąbek et al, 2017;Dąbek et al, 2019). Cleaned material of H. ostrearia (voucher strain no.…”

“…The 'Golden era' in South African marine diatomology was associated with the research of Professor Malcolm Giffen (1902Giffen ( -1986, who produced numerous papers on marine and estuarine benthic diatoms of the Indian Ocean, south coast in the Eastern Cape Province (Giffen, 1963;Giffen, 1966;Giffen, 1970a) and the Atlantic Ocean, west coast between Gordon's Bay and Namaqualand (Giffen, 1970b;Giffen, 1971;Giffen, 1973;Giffen, 1975;Giffen, 1976;Giffen, 1984). More recently, marine benthic diatom research was focused on taxonomy and phylogeny (e.g., Dąbek et al, 2013;Dąbek et al, 2014;Dąbek et al, 2019), local diversity and ecology (Cotiyane-Pondo et al, 2020;Cotiyane-Pondo and Bornman, 2021a;Cotiyane-Pondo et al, 2021b) or epiphytic, epibiontic host-diatoms relationships (Majewska et al, 2019a;Majewska et al, 2019b;Majewska, 2020;Mayombo et al, 2020;Majewska et al, 2022).…”