Toward a point-of-care diagnostic for specific detection of Mycobacterium tuberculosis from sputum samples

Eloi, P.; Nascimento, G.A.; Córdula, C.; Visani, Valeria; Castelletti, H.; Bezerra, Giselda; Soares, Luana Silva; Lima, Batista; Bruneska, Danyelly; Montenegro, Lílian Maria Lapa; Schindler, Haiana Charifker; Cavalcanti, Isabella Macário Ferro; Campos-Ferreira, Danielly S.; Lima-Filho, José Luiz de

doi:10.1016/j.tube.2020.101919

Cited by 14 publications

(8 citation statements)

References 36 publications

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“…Nevertheless, the effective detection of clinical samples is a key for further application of the designed RPA technology (Eloi et al . 2020). Subsequently, a total of 120 clinical sputum samples from the patients were collected for the detection and evaluation, the basic information of these participant was concluded in Table .…”

Section: Resultsmentioning

confidence: 99%

Rapid detection of Mycobacterium tuberculosis based on antigen 85B via real-time recombinase polymerase amplification

Zhang

et al. 2021

Letters in Applied Microbiology

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Tuberculosis (TB), as a common infectious disease, still remains a severe challenge to public health. Due to the unsatisfied clinical needs of currently available diagnostic vehicles, it is desired to establish a new approach for universally detecting Mycobacterium tuberculosis. Herein, we designed a real‐time recombinase polymerase amplification (RPA) technology for identifying M. tuberculosis within 20 min at 39°C via custom‐designed oligonucleotide primers and probe, which could specifically target antigen 85B (Ag85B). Particularly, the primers F4‐R4 produced the fastest fluorescence signal with the probe among four pairs of designed primers in the RPA assays. The optimal primers/probe combination could effectively identify M. tuberculosis with the detection limit of 4·0 copies per μl, as it could not show a positive signal for the genomic DNA from other mycobacteria or pathogens. The Ag85B‐based RPA could determine the genomic DNA extracted from M. tuberculosis with high reliability (100%, 22/22). More importantly, when testing clinical sputum samples, the real‐time RPA displayed an admirable sensitivity (90%, 95% CI: 80·0‐96·0%) and specificity (98%, 95% CI: 89·0‐100·0%) compared to traditional smear microscopy, which was similar to the assay of Xpert MTB/RIF. This real‐time RPA based Ag85B provides a promising strategy for the rapid and universal diagnosis of TB.

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Section: Resultsmentioning

confidence: 99%

Rapid detection of Mycobacterium tuberculosis based on antigen 85B via real-time recombinase polymerase amplification

Zhang

et al. 2021

Letters in Applied Microbiology

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“…The IS6110 gene is found at various sites in the MTB genome. Thus, this gene sequence detection was helpful for MTB diagnosis, and the DPV-based electrochemical technique was widely used to monitor the IS6110 gene [ [124] , [125] , [126] , [127] ]. Arginine contained an active amino group (−NH 2 ), providing an excellent platform for hybridization between the probe DNA and target DNA.…”

Section: Electrochemical Biosensors For Airborne Pathogen Detectionmentioning

confidence: 99%

Recent advances in airborne pathogen detection using optical and electrochemical biosensors

Sivakumar

Lee

2022

Analytica Chimica Acta

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“…Yet another PCR-free alternative for TB diagnostics, biosensors containing arginine film, or a graphene-based sensor which, when combined with Mtb based probes have the ability to perform electrochemical measurements following hybridisation at room temperature; hence can be easily adopted in rural areas with a portable power supply. 103 copies of DNA are defined as the detection limit of this method, which limits its role in identifying latent, immunocompromised, HIV and paediatric patients (Eloi et al, 2020;Jaroenram et al, 2020).…”

Section: Graphene-based Biosensorsmentioning

confidence: 99%

Tuberculosis Diagnostics, a journey from the past Experiences to the Future Directions, Review

Moorthy

Bhaskar

Shivakumar³

et al. 2022

AGJSR

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Purpose: Tuberculosis caused by Mycobacterium tuberculosis is believed to have been acquired from animals especially cattle. Not one country across the globe is spared of the disease. The infection gets established through respiratory system where they are either latent or active. Depending upon the immune status or coexisting infection or other health disorders the infection may be either contained within the lungs or spread to other parts of the body. Hence rapid and accurate diagnosis is needed to initiate appropriate treatment. Sputum studies are being followed for diagnosis of tuberculosis but has few disadvantages. Xpert MTB/RIF, an automated, molecular test has been in vogue now. Biomarkers such as complete blood count, inflammatory and oxidative stress markers are being measured. Recently epigenetic markers like microRNA are being analyzed to differentiate the various presentations of tuberculosis. This review has been undertaken to understand the various diagnostic strategies being followed from the old times to the newer novel techniques. Methods: The narrative review was undertaken by searching the original and review articles in the past 20 years. The articles were obtained by searching through various search engines. Since this was a review article ethics committee approval was not required. Results: The traditional methods have inherent limitations of high expertise, prolonged procedure, not cost effective and require good infrastructure. Newer methods are found to be sensitive, specific, reproducible and cost effective. But require validation to be implemented into routine use. Conclusions: Validating the new markers such as epigenetic markers – microRNAs should pave way for diagnosis and management of pulmonary TB, extrapulmonary TB, TB in HIV infected individuals, pregnant women and children. This could serve as a finger-print sort of diagnosis once validated on large scale community-based studies.

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Toward a point-of-care diagnostic for specific detection of Mycobacterium tuberculosis from sputum samples

Cited by 14 publications

References 36 publications

Rapid detection of Mycobacterium tuberculosis based on antigen 85B via real-time recombinase polymerase amplification

Rapid detection of Mycobacterium tuberculosis based on antigen 85B via real-time recombinase polymerase amplification

Recent advances in airborne pathogen detection using optical and electrochemical biosensors

Tuberculosis Diagnostics, a journey from the past Experiences to the Future Directions, Review

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