Toward an objective diagnostic for detection of <i>Leishmania RNA virus</i> (LRV) in clinical samples from cutaneous leishmaniasis patients

Parra-Muñoz, Marcela; Aponte, Samanda; Ovalle-Bracho, Clemencia; Ch, Saavedra; Mc, Echeverry

doi:10.1101/338095

2018

DOI: 10.1101/338095

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Toward an objective diagnostic for detection of Leishmania RNA virus (LRV) in clinical samples from cutaneous leishmaniasis patients

Marcela Parra-Muñoz

Samanda Aponte

Clemencia Ovalle-Bracho

et al.

Abstract: 18Leishmania RNA virus (LRV) is a double strand RNA virus belonging to the was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/338095 doi: bioRxiv preprint first posted online Jun. 4, 2018; 2 and treatment failure of cutaneous leishmaniasis (TFCL). However, the reported

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Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

et al. 2019

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Background Leishmania RNA virus-1 (LRV1) is a double-stranded RNA virus identified in 20–25% of Viannia —species endemic to Latin America, and is believed to accelerate cutaneous to mucosal leishmaniasis over time. Our objective was to quantify known virulence factor (VF) RNA transcript expression according to LRV1 status, causative species, and isolate source. Methods Eight cultured isolates of Leishmania were used, four of which were LRV1-positive ( Leishmania Viannia braziliensis [ n = 1], L . ( V .) guyanensis [ n = 1], L . ( V .) panamensis [ n = 2]), and four were LRV1-negative ( L . ( V .) panamensis [ n = 3], L . ( V .) braziliensis [ n = 1]). Promastigotes were inoculated into macrophage cultures, and harvested at 24 and 48 h. RNA transcript expression of hsp23 , hsp70 , hsp90 , hsp100 , mpi , cpb , and gp63 were quantified by qPCR. Results RNA transcript expression of hsp100 ( p = 0.012), cpb ( p = 0.016), and mpi ( p = 0.022) showed significant increases from baseline pure culture expression to 24- and 48-h post-macrophage infection, whereas hsp70 ( p = 0.004) was significantly decreased. A trend toward increased transcript expression of hsp100 at baseline in isolates of L . ( V .) panamensis was noted. Pooled VF RNA transcript expression by L . ( V .) panamensis isolates was lower than that of L . ( V .) braziliensis and L . ( V .) guyananesis at 24 h ( p = 0.03). VF RNA transcript expression did not differ by LRV1 status, or source of cultured isolate at baseline, 24, or 48 h; however, a trend toward increased VF RNA transcript expression of 2.71- and 1.93-fold change of mpi ( ...

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Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

et al. 2019

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Toward an objective diagnostic for detection of Leishmania RNA virus (LRV) in clinical samples from cutaneous leishmaniasis patients

Cited by 1 publication

References 55 publications

Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

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