Toward An Optimum Return From Crop Plants

Song, Xinbo; Ashikari, Motoyuki

doi:10.1007/s12284-008-9018-3

Cited by 16 publications

(7 citation statements)

References 84 publications

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“…Cereals are the most important source of food, therefore higher production in crop plants may prove to be necessary to satisfy the increasing demand in food. Rice yield potential is determined by several factors including seed size (or weight), number of panicles per plant and number of seeds per panicle (Takeda and Matsuoka, 2008;Song and Ashikari, 2008). Among these factors, we have been focusing on the study of seed size and continue to identify regulatory genes (Fujisawa et al, 1999;Tanabe et al, 2005;Kitagawa et al, 2010), in order to understand the molecular mechanisms that underline seed size regulation.…”

Section: Introductionmentioning

confidence: 99%

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

Abe

Mieda

Ando³

et al. 2010

Genes Genet. Syst.

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The causal gene of a novel small and round seed mutant 1 (srs1) was identified in rice by map-based cloning and named SMALL AND ROUND SEED 1 (SRS1). The SRS1 gene is identical to the previously identified DENSE AND ERECT PANICLE 2 (DEP2). The SRS1/DEP2 gene encodes a novel protein of 1365 amino acids residues without known functional domains. In the longitudinal direction of the lemma, both cell length and cell number are reduced in srs1-1 compared to the wild type, whereas in the lateral cross section of the lemma, cell length in srs1-1 is greater than that in the wild type, but the cell number in srs1-1 is the same as that in wild type. These results suggest that the small and round seed phenotype of srs1-1 is due to the reduction in both cell length and cell number in the longitudinal direction, and the elongation of the cells in the lateral direction of the lemma. The SRS1 mRNA and proteins are abundant in wild type rice specifically in young organs, namely young leaves, internodes and panicles. Interestingly, the tissues expressing SRS1 are closely related to the tissues that exhibit abnormalities in the srs1 mutants.

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Section: Introductionmentioning

confidence: 99%

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

Abe

Mieda

Ando³

et al. 2010

Genes Genet. Syst.

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show abstract

“…To date, over 400 QTLs modulating grain size and weight have been identi ed and are distributed on each of rice's 12 chromosomes Zuo and Li 2014;Kashifet al 2020). However, only a few major QTLs including GS3, qSW5,GW2,qGL3/GL3.1,GW8, GL7/GW7, TGW6and GS9 have been isolated by map-based cloning methods (Mao et al 2010;Shomura et al 2008;Song et al 2007;Qi et al 2012;Wang et al 2012;Wang et al 2015a;Wang et al 2015b;Ishimaru et al 2013;Zhao et al 2018).The isolation of these genes has enhanced ourknowledge of the molecular regulatory mechanisms responsible for grain size and weight (Song et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Validation of a QTL for grain size and weight using an introgression line from a cross between Oryza sativa and Oryza minuta 

Feng

Yuan

Wang

et al. 2021

Preprint

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Background: Grain size and weight are important target traits determining grain yield and quality in rice. Wild rice species possess substantial elite genes that can be served as an important resource for genetic improvement of rice. In this study, we identify and validate a novel QTL on chromosome 7 affecting the grain size and weight using introgression lines from cross of Oryza sativa and Oryza minuta. Results: An introgression line ‘IL188’ has been achieved from a wild species Oryza minuta (2n = 48, BBCC, W303) into O. sativa japonica Nipponbare. The F2 and F2:3 populations derived from a cross between IL188 and Nipponbare were used to map QTLs for five grain size traits, including grain length (GL), grain width (GW), grain length to width ratio (LWR), grain thickness (GT) and thousand grain weight (TGW). A total of 12 QTLs for the five grain traits were identified on chromosomes 1, 2, 3, 6, 7, and 8. The QTL-qGL7 controlling GL on chromosome 7 was detected stably in the F2 and F2:3 populations, and explained 15.09-16.30% of the phenotypic variance. To validate the effect of qGL7, eight residual heterozygous line (RHL) populations were developed through selfing four F2:3 and four F2:4 plants with different heterozygous segments for the target region. By further developing SSR and Indel markers in the target interval, qGL7 was delimited to a ~261 kb region between Indel marker Y7-12 and SSR marker Y7-38, which also showed significant effects on grain width and thousand grain weight. Comparing with the reference genome of Nipponbare, stop or frameshift mutations in the exon of the three putative genes LOC_Os07g36830, LOC_Os07g36900 and LOC_Os07g36910 encoding F-box domain-containing proteins may be the candidate genes for qGL7. Scanning electron microscopy analysis of the glume's epidermal cells showed that the cell length and width of NIL-qGL7IL188 was higher than NIL-qGL7Nip, indicating that qGL7 increases grain size and weight by regulating cell expansion. Conclusions: In this study, we detected 12 QTLs regulating grain size and weight using an introgression line from a cross between an Oryza sativa and Oryza minuta. Of these loci, we confirmed and delimited the qGL7 to a ~261 kb region. Three putative genes, LOC_Os07g36830, LOC_Os07g36900 and LOC_Os07g36910 encoding F-box domain-containing proteins may be the candidate genes for qGL7. These results provide a basis for map-based cloning of the qGL7 gene and useful information for marker assisted selection in rice grain quality improvement.

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“…The isolation of these genes has enhanced our knowledge of the molecular regulatory mechanisms responsible for grain size and weight (Song et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Validation of a QTL for Grain Size and Weight Using an Introgression Line from a Cross Between Oryza Sativa and Oryza Minuta

Feng

Yuan

Wang

et al. 2020

Preprint

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Background: Grain size and weight are important target traits determining grain yield and quality in rice. Wild rice species possess substantial elite genes that can be served as an important resource for genetic improvement of rice. In this study, we identify and validate a novel QTL on chromosome 7 affecting the grain size and weight using introgression lines from cross of Oryza sativa and Oryza minuta.Results: An introgression line ‘IL188’ has been achieved from a wild species Oryza minuta (2n = 48, BBCC, W303) into O. sativa japonica Nipponbare. The F2 and F2:3 populations derived from a cross between IL188 and Nipponbare were used to map QTLs for five grain size traits. A total of 12 QTLs for the five grain traits were identified on chromosomes 1, 2, 3, 6, 7, and 8. The QTL qGL7 on chromosome 7 was detected stably in the F2 and F2:3 populations, and explained 15.09-16.30% of the phenotypic variance. To validate the effect of qGL7, eight residual heterozygous line (RHL) populations were developed through selfing four F2:3 and four F2:4 plants with different heterozygous segments for the target region. By further developing SSR and Indel markers in the target interval, qGL7 was delimited to a ~261 kb region between Indel marker Y7-12 and SSR marker Y7-38, which also showed significant effects on grain width and thousand grain weight. Scanning electron microscopy analysis of the glume's epidermal cells showed that the cell length and width of NIL-qGL7IL188 was higher than NIL-qGL7Nip, indicating that qGL7 increases grain size and weight by regulating cell expansion. Conclusions: In this study, we detected 12 QTLs regulating grain size and weight using an introgression line from a cross between an Oryza sativa and Oryza minuta. Of these loci, we confirmed and delimited the qGL7 to a ~261 kb region. These results provide a basis for map-based cloning of the qGL7 gene and useful information for marker assisted selection in rice grain quality improvement.

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Toward An Optimum Return From Crop Plants

Cited by 16 publications

References 84 publications

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

Validation of a QTL for grain size and weight using an introgression line from a cross between Oryza sativa and Oryza minuta

Validation of a QTL for Grain Size and Weight Using an Introgression Line from a Cross Between Oryza Sativa and Oryza Minuta

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Toward An Optimum Return From Crop Plants

Cited by 16 publications

References 84 publications

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice

Validation of&nbsp;a&nbsp;QTL&nbsp;for&nbsp;grain&nbsp;size&nbsp;and weight&nbsp;using an introgression line from&nbsp;a cross between&nbsp;Oryza&nbsp;sativa&nbsp;and&nbsp;Oryza&nbsp;minuta&nbsp;

Validation of a QTL for Grain Size and Weight Using an Introgression Line from a Cross Between Oryza Sativa and Oryza Minuta

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Validation of a QTL for grain size and weight using an introgression line from a cross between Oryza sativa and Oryza minuta