Toward Cell Selective Surfaces: Cell Adhesion and Proliferation on Breath Figures with Antifouling Surface Chemistry

Abstract

We report the preparation of microporous functional polymer surfaces that have been proven to be selective surfaces toward eukaryotic cells while maintaining antifouling properties against bacteria. The fabrication of functional porous films has been carried out by the breath figures approach that allowed us to create porous interfaces with either poly(ethylene glycol) methyl ether methacrylate (PEGMA) or 2,3,4,5,6-pentafluorostyrene (5FS). For this purpose, blends of block copolymers in a polystyrene homopolymer matrix have been employed. In contrast to the case of single functional polymer, using blends enables us to vary the chemical distribution of the functional groups inside and outside the formed pores. In particular, fluorinated groups were positioned at the edges while the hydrophilic PEGMA groups were selectively located inside the pores, as demonstrated by TOF-SIMS. More interestingly, studies of cell adhesion, growth, and proliferation on these surfaces confirmed that PEGMA functionalized interfaces are excellent candidates to selectively allow cell growth and proliferation while maintaining antifouling properties.

Fabrication of biocompatible and efficient antimicrobial porous polymer surfaces by the Breath Figures approach

“…Biological evaluation was performed as described in [34]. Briefly, the microporous polymer surfaces were sterilized rinsing with ethanol and by UV radiation exposure.…”

“…The antibacterial activity of the microporous surfaces was investigated using S. aureus (strain RN4220) adapting a previously reported protocol. [34] The bacteria were genetically modified with plasmid pCN57 in order to express green fluorescent protein (GFP) (grown in Luria-Bertani (LB) media with erythromycin (10 μg mL −1 ) overnight at 37°C). Prior to the deposition onto the polymeric surfaces, the bacterial cells were washed three times in PBS saline buffer (150 mM NaCl, phosphate 50 mM pH 7.4) and centrifuged.…”

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices

“…Plentiful pores, the inherent structural characteristics of bone matrix which are inherited by DCDBMs and HPTBMs may enhance the adhesion and infiltration of reseeded cells . Actually, the superior cell adhesion and infiltration were observed in both two materials.…”

Breath-Figures Self-Assembly, the Versatile Method of Manufacturing Membranes and Porous Structures: Physical, Chemical and Technological Aspects

“…A number of very recent applications of these structures are noteworthy. Microporous functional polymer surfaces, arising from the breath-figures self-assembly have been proven to be selective surfaces toward eukaryotic cells while maintaining antifouling properties against bacteria [168]. The witty process of manufacturing of micro-spherical particles with the breath-figures process was reported in Ref.…”