Toward Personalized Cell Therapies by Using Stem Cells: Seven Relevant Topics for Safety and Success in Stem Cell Therapy

Abstract

Stem cells, both embryonic and adult, due to the potential for application in tissue regeneration have been the target of interest to the world scientific community. In fact, stem cells can be considered revolutionary in the field of medicine, especially in the treatment of a wide range of human diseases. However, caution is needed in the clinical application of such cells and this is an issue that demands more studies. This paper will discuss some controversial issues of importance for achieving cell therapy safety and success. Particularly, the following aspects of stem cell biology will be presented: methods for stem cells culture, teratogenic or tumorigenic potential, cellular dose, proliferation, senescence, karyotyping, and immunosuppressive activity.

Proposing the use of dental pulp stem cells as a suitable biological model of neurofibromatosis type 1

“…Adult stem cells, in general, are regarded as a useful model for various diseases [9], supporting the possibility of their use as a model for studying NF1 and predicting earlier in the carrier's life which alterations will probably appear in individuals. Dental pulp stem cells (DPSCs) are particularly useful since they can be isolated from an easily extracted tissue [10], which is available for harvesting during the individual's early life, coinciding with the period when the hyperpigmentation spots, used as a part of the clinical diagnosis, appear.…”

Stem cell–based therapies for the newborn lung and brain: Possibilities and challenges

“…In addition, they do not carry the teratogenic potential of ES cells after in vivo transplantation 23 . MSCs are also thought to be immunoprivileged in that they evade clearance by the recipient immune system 24 due to low expression of the major histocompatibility complexes and to their ability to inhibit proliferation and function of immune cells, such as dendritic cells, NK cells, and T and B lymphocytes 25-27 .…”

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

“…One such difficulty was detected in studies of animal models and in humans, where stem cell engraftment was quantified after injection and showed that only a small percentage of cells remained viable after days or weeks. Thus, there is a need for having a high number of stem cells before transplantation [45], which can be obtained by PBMT of cells. Another procedure able to get these amounts of stem cells is the Bcell sheet^technology.…”