2013
DOI: 10.1007/s13238-013-3074-1
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Toward pluripotency by reprogramming: mechanisms and application

Abstract: The somatic epigenome can be reprogrammed to a pluripotent state by a combination of transcription factors. Altering cell fate involves transcription factors cooperation, epigenetic reconfi guration, such as DNA methylation and histone modification, posttranscriptional regulation by microRNAs, and so on. Nevertheless, such reprogramming is inefficient. Evidence suggests that during the early stage of reprogramming, the process is stochastic, but by the late stage, it is deterministic. In addition to convention… Show more

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Cited by 19 publications
(15 citation statements)
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“…515 Consistent with this, alterations in methylation-dependent processes are associated with cancer, 16 neurodegenerative/neuropsychiatric disorders, 1719 inflammation, 20,21 metabolic disorders, 22 fundamental development/regenerative medicine, 23,24 susceptibility to disease/adverse drug response, 25,26 and drug resistance. 1315,27,28 While there have been great advances in methylation-dependent bioinformatics and disease-associated biomarkers, 2931 the study of intracellular MT spatial/temporal resolution, specificity, and/or function remains a challenge.…”
mentioning
confidence: 62%
“…515 Consistent with this, alterations in methylation-dependent processes are associated with cancer, 16 neurodegenerative/neuropsychiatric disorders, 1719 inflammation, 20,21 metabolic disorders, 22 fundamental development/regenerative medicine, 23,24 susceptibility to disease/adverse drug response, 25,26 and drug resistance. 1315,27,28 While there have been great advances in methylation-dependent bioinformatics and disease-associated biomarkers, 2931 the study of intracellular MT spatial/temporal resolution, specificity, and/or function remains a challenge.…”
mentioning
confidence: 62%
“…Several factors introduce variability and affect the ability to compare data from multiple studies, including patient differences, iPSC reprogramming methods, and neuronal differentiation paradigms. Inherent genetic variation among individuals due to genetic diversity, variability of iPSC clones from a single individual, as well as disease presentation presents major challenges to iPSC disease modeling [51][52][53]. Epigenetic and copy number diversity adds another layer of complexity [54] that may plague iPSCs to a greater extent than other samples.…”
Section: Discussionmentioning
confidence: 99%
“…Commonly used factors include the so-called Yamanaka factors, Oct4, KLF4, Sox2, and c-Myc (Takahashi and Yamanaka, 2006), although alternative transcription factors, microRNAs, or chemical factors have also been used (Wang et al, 2013). Only transient over expression of stem cell factors is required before the endogenous network is sufficiently activated, allowing the use of non-integrating approaches, including non-integrating viruses, episomal overexpression, and modified RNA among others (Hu, 2014).…”
Section: Cellular Reprogramming Of Somatic Cells To Ipscsmentioning
confidence: 99%
“…Typically skin fibroblasts or peripheral blood are used as starting materials as they are easily acquired from patient donation and biorepositories. The precise molecular mechanisms underlying pluripotent reprogramming are still not fully understood, but seem to involve an early stochastic process that becomes increasingly deterministic as the endogenous stem cell transcriptional network is activated, as has been reviewed elsewhere (Hanna et al, 2009;Wang et al, 2013).…”
Section: Cellular Reprogramming Of Somatic Cells To Ipscsmentioning
confidence: 99%