Toward reconstruction of the subcutaneous fat layer with the use of adipose-derived stromal cell–seeded collagen matrices

Werner, Katharina; Jakubietz, Michael G.; Jakubietz, Rafael G.; Schmidt, Karsten; Muhr, Christian; Bauer‐Kreisel, Petra; Blunk, Torsten

doi:10.1016/j.jcyt.2014.06.007

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…Further studies in preclinical models are needed to investigate whether the cell-instructive scaffolds can function in combination with intrinsic factors following in vivo implantation to guide the further differentiation of the cells within the constructs according to the local needs, as shown in principle in previous studies. 46−48 Fiber structures as generated here may benefit the reconstruction of subcutaneous adipose tissue, 49 and recently, we have combined similar MEW fiber constructs with spheroids made from ASCs as a tissue building block. 8 In that study, however, only unmodified PCL scaffolds were used and only standard long-term induction was performed.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Matrix-Modified Fiber Scaffolds as a Proadipogenic Mesenchymal Stromal Cell Delivery Platform

Blum

Schlegelmilch

Schilling

et al. 2019

ACS Biomater. Sci. Eng.

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Melt electrowriting (MEW) is an additive manufacturing technology that produces readily handleable fibrous scaffolds with controlled geometry to support cell infiltration. Although MEW scaffolds have excellent potential for cell delivery in regenerative medicine applications, studies to date have primarily focused on polymers such as poly(ε-caprolactone) (PCL) that lack bioactive cues to affect cell function. To address this aspect, MEW scaffolds with extracellular matrix (ECM) coatings were developed as a proadipogenic platform for human mesenchymal stromal cells (hMSCs). More specifically, highly flexible PCL scaffolds fabricated through MEW were coated with a complex ECM suspension prepared from human decellularized adipose tissue (DAT), purified fibronectin, or laminin to determine the effects of two key bioactive proteins present within adipose-derived ECM. In vitro studies exploring the response of human bone marrow-derived mesenchymal stromal cells cultured under adipogenic differentiation conditions indicated a high level of differentiation on all substrates studied, including unmodified PCL scaffolds and two-dimensional controls. To more fully assess the intrinsic proadipogenic capacity of the composite biomaterials, a modified culture regime was established that involved a short-term adipogenic induction in differentiation medium, followed by continued culture in maintenance medium supplemented with insulin for up to 3 weeks. Under these conditions, adipogenic differentiation was enhanced on all fiber scaffolds as compared to the tissue culture controls. Notably, the highest adipogenic response was consistently observed on the PCL + DAT scaffolds, based on the analysis of multiple markers including adipogenic gene [lipoprotein lipase, fatty acid binding protein 4 (FABP4), adiponectin, perilipin 1] and protein (FABP4, leptin) expression and intracellular triglyceride accumulation. Taken together, the PCL scaffolds incorporating DAT provide an adipoinductive microenvironment for the hMSCs, with particular applicability of this cell-instructive delivery platform for applications in plastic and reconstructive surgery.

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Section: Discussionmentioning

confidence: 99%

Extracellular Matrix-Modified Fiber Scaffolds as a Proadipogenic Mesenchymal Stromal Cell Delivery Platform

Blum

Schlegelmilch

Schilling

et al. 2019

ACS Biomater. Sci. Eng.

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“…Sheet‐like structures of engineered adipose tissue are desirable in reconstructive surgery, as, for example, many cases of complex injuries of the upper and the lower extremities leading to excessive scarring would benefit from the reconstruction of the subcutaneous fat layer . Thus far, preformed ASC spheroids have been scarcely used in adipose engineering, and appropriate spheroid delivery remains challenging.…”

Section: Mew Parameters and Corresponding Scaffold Dimensions Determimentioning

confidence: 99%

Tailored Melt Electrowritten Scaffolds for the Generation of Sheet‐Like Tissue Constructs from Multicellular Spheroids

McMaster

Hoefner

Hrynevich

et al. 2019

Adv Healthcare Materials

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“…As illustrated before with full thickness skin models, attempts to reconstruct SC tissue using various approaches and techniques were realized these last years and were reviewed elsewhere [156][157][158]. All of these studies were aiming to regenerate soft tissue in order to provide a cushioning layer for wound healing or to restore volume after resection of tumors, like mastectomy [159][160][161]. Common 745 basis is the use of synthetic or natural biomaterial(s) as scaffolds, on top of, or into which are seeded human ASC isolated from (autologous) liposuction.…”

Section: Subcutaneous Tissue Models 710mentioning

confidence: 99%

In vitro models for immunogenicity prediction of therapeutic proteins

Groell

Jordan

Borchard

2018

European Journal of Pharmaceutics and Biopharmaceutics

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Immunogenicity assessment of therapeutic proteins is routinely performed through various techniques during the drug development process: (i) in silico to design the least immunogenic protein possible, (ii) in vitro using mainly classic 2D assays with PBMC-derived cells or immune cell lines to follow protein uptake, immune cell maturation and pro-inflammatory cytokines released, (iii) in vitro using 3D models of the human immune lymphatic system or full-thickness skin, (iv) and finally in vivo with preclinical and clinical studies. This review focuses primarily on the immunogenicity assessment of therapeutic proteins injected subcutaneously and new in vitro models that may be used as specific models of this tissue.

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Toward reconstruction of the subcutaneous fat layer with the use of adipose-derived stromal cell–seeded collagen matrices

Cited by 4 publications

References 26 publications

Extracellular Matrix-Modified Fiber Scaffolds as a Proadipogenic Mesenchymal Stromal Cell Delivery Platform

Extracellular Matrix-Modified Fiber Scaffolds as a Proadipogenic Mesenchymal Stromal Cell Delivery Platform

Tailored Melt Electrowritten Scaffolds for the Generation of Sheet‐Like Tissue Constructs from Multicellular Spheroids

In vitro models for immunogenicity prediction of therapeutic proteins

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